Separation and semi-quantitative determination of tetracycline degradation products in tetracycline hydrochloride powders and capsules by thin-layer chomatography

Omer, A.I.H.; Kariem, Elrasheed A. Gad; Salama, R.B.

doi:10.1016/s0021-9673(00)82683-7

Cited by 11 publications

(2 citation statements)

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“…Fluorescence emission has been used by several researchers 14,20,[33][34][35][36] to analyze tetracyclines. Most researchers have used enhanced fluorescence signals by chelating TCs with metallic ions.…”

Section: Fluorometrymentioning

confidence: 99%

“…33,37 However, any modification of the molecules may decrease the resolution in TLC analysis. The natural fluorescence properties of TCs have been used for qualitative 20,35 and semi-quantitative analysis 34,36 on TLC plates, but no detailed information could be found regarding the fluorescence properties of TC and its possible impurities. Therefore, an experiment was performed to investigate the fluorescence properties of TCs, using methanol solutions of TC and its impurities for fluorometric analysis.…”

Section: Fluorometrymentioning

confidence: 99%

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Utilization of a scientifically operated charge-coupled device detector for high-performance thin-layer chromatographic analysis of tetracyclines

Liang

Simon

Denton³

1999

Analyst

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A high-performance thin-layer chromatography (HPTLC) method using a scientifically operated charge-coupled device detector is described for the assay of tetracycline pharmaceutical products. Quantitative information can be obtained for all samples on a TLC plate within a few seconds. The separation efficiency and detection limits were determined on both normal phase and reverse phase TLC plates. Fluorescence detection mode offers higher sensitivity than fluorescence quenching mode. The dynamic range, sensitivity, accuracy and precision of the system were evaluated. Detection limits of the impurities are in the range of 0.1 to 0.5 ng or 0.3 to 1% of tetracycline, depending on the compound, with a recovery percentage over 85%. The existing impurities in tetracycline capsules were determined using both HPLC and HPTLC techniques. All of the impurities were below the regulation level.

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