Improvement of the chemical analysis of antibiotics I. Simple method for the analysis of tetracyclines on silica gel high-performance thin-layer plates

Oka, Hisao; Uno, Keiichi; Harada, Kenichi; Kaneyama, Y.; Suzuki, Makoto

doi:10.1016/0021-9673(83)80053-3

Cited by 22 publications

(10 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HPLC analyses was performed using Shimadzu HPLC machine (Schimadzu Coporation, Kyoto, Japan). For mobile phase, HPLC separation of Pb, and Co residues were carried out at a 1:1.5:5 mixture of methanolacetonitrile (0.01 M), aqueous oxalic acid solution and oxalic acid was modified with ammonia solution of 2.0 pH according to procedure earlier described by Oka and Uno (1984) with the flow rate of 0.8 mL/min. (Karno and Sean, 2003).…”

Section: Laboratory Analyses Of Heavy Metals Using H I G H P E R F O mentioning

confidence: 99%

“…(Karno and Sean, 2003). Regarding the standard Stocks for Pb and Co elements, the standard stock solutions for mobile phase were formulated by precision weighing of 20 mg each of Pb and Co reference analogues using digital scale and immediately dissolving them each in 10 mL of methanol (Onasanya et al, 2019b;Oka and Uno, 1984). The standard stock solutions of the Pb and Co heavy metals were subsequently stored in refrigerator for further analyses.…”

Section: Laboratory Analyses Of Heavy Metals Using H I G H P E R F O mentioning

confidence: 99%

“…Thereafter, the working stocks were subsequently prepared from the previously constituted stock solutions by diluting it with appropriate volume of methanol to make total concentrations range of 0.0092-1.1 mg/mL. The residues of heavy metals were successfully optimised to identify and separate these heavy metal residues and other organic compounds in a biological matrix of locally processed yoghurt sample, by spiking 0.2 mg/mL each of heavy metal analogues with 10 mL of locally processed yoghurt (Onasanya et al, 2019b;Oka and Uno, 1984). The locally processed yoghurt samples were later acidified with 1 mL of TFA and centrifuged for 15 minute using 0.2 µm syringe filter and subsequently injected directly into the supernatant liquid which was subsequently drawn in the HPLC system for appropriate identification and separation (Onasanya et al, 2019b;Oka and Uno, 1984).…”

Section: Laboratory Analyses Of Heavy Metals Using H I G H P E R F O mentioning

confidence: 99%

“…Evaluation of the specific rotation was accomplished using the peak height method as previously reported by (Rice et al, 1989). Regarding the separation of heavy metals analogue (Pb and Co) and other organic compound, HPLC-UV detection system was performed out according to earlier published works of Onasanya et al (2019b) and Oka and Uno (1984). The UV detector of the Schinadzu HPLC was visualised in millivolts (Onasanya et al, 2019b).…”

Section: Laboratory Analyses Of Heavy Metals Using H I G H P E R F O mentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of lead and cobalt residues in locally processed yoghurt from native dairy cattle raised under traditional system of management

Onasanya¹,

Umar²,

Umar³

et al. 2020

NJAP

View full text Add to dashboard Cite

Heavy metals are partitioned into two different groups of essential (non-toxic) and nonessential (toxic) elements. The non-toxic heavy metals are of essential health and nutritional benefits to animals and humans. The clinical consequences of non-essential heavy metals if ingested even in low doses are grave. This study was the concluding part of our previous works on the detection of drug residues and bacterial contaminations of locally processed yoghurt sold for human consumption in Jigawa State of Northern Nigeria. In this present study, we attempted to evaluate the presence of Lead (Pb) and Cobalt (Co) heavy metal residue in samples of locally processed yoghurt hawked for human consumption in selected parts of Jigawa State, Nigeria. About 10 mL each of four samples of locally processed yoghurt samples were randomly collected from each of five collection centers/points in the morning from large containers into sterilized collection tubes and cooled and transported in iced pack box for laboratory analyses. The evaluation of heavy metal residues was performed using high performance liquid chromatography (HPLC)-based assay. We presented from our results that sample of locally processed yoghurt collected from Kiri had the highest Cobalt (Co) residue (75.18 mg/kg) followed by Majiyawa (65.84 mg/kg) both of which were from Ringim dairy collection center while Dakayyawa, Andaza and Balbadu dairy collection centers had Co residue-free yoghurt. Majority of the locally processed yoghurt samples collected from the study area had elevated Pb contamination levels which were far higher than those collected from Rumbawa (0.00 mg/kg), Dakayyawa (0.00 mg/kg), Kyambo (0.02 mg/kg), Dangyati (0.02 mg/kg) and Andaza (0.02 mg/kg) dairy collection centers/points. Our results suggested that Pb contamination levels detected in the study area is clinically unsafe and unhygienic for human consumption and it is of grave consequences to public health. Therefore, urgent action is needed to be taken by appropriate agency of government to address this health threat to the consumers' safety of dairy products in the study area. Les métaux lourds sont répartis en deux groupes différents d'éléments essentiels (non toxiques) et non essentiels (toxiques). Les métaux lourds non toxiques présentent des avantages essentiels pour la santé et la nutrition des animaux et des humains. Les conséquences cliniques des métaux lourds non essentiels en cas d'ingestion même à dosesfaibles sont graves. Cette étude était la conclusion de nos travaux antérieurs sur la détection des résidus de médicaments et des contaminations bactériennes de yaourts transformés localement vendus pour la consommation humaine dans l'État de Jigawa au nord du Nigéria. Dans l'étude actuelle, nous avons tenté d'évaluer la présence de résidus de métaux lourds de plomb (Pb) et de cobalt (Co) dans des échantillons de yaourt transformé localement destiné à la consommation humaine dans certaines régions de l'État de Jigawa, au Nigéria. Environ 10 ml de chacun des quatre échantillons d'échantillons de yaourt transfor és localement ont été prélevés au hasard dans chacun des cinq centres / points de collecte le matin à partir de grands conteneurs dans des tubes de collecte stérilisés et refroidis et transportés dans une boîte d'emballage glacée pour les analyses de laboratoire. L'évaluation des résidus de métaux lourds a été réalisée en utilisant un test basé sur la chromatographie liquide à haute performance (HPLC). Nous avons présenté à partir de nos résultats que l'échantillon de yaourt transformé localement recueilli à Kiri contenait le résidu de cobalt (Co) le plus élevé (75.18 mg / kg) suivi de Majiyawa (65.84 mg / kg), tous deux provenant du centre de collecte laitière de Ringim tandis que Dakayyawa, Andaza et les centr s de collecte des produits laitiers de Balbadu disposaient de yaourt sans résidu de Co. La ma orité des échantillons de yaourt transformés localement prélevés dans la zone d'étude présentaient des niveaux de contamination élevés au plomb qui étaient bien supérieurs à ceux recueillis à Rumbawa (0.00 mg / kg), Dakayyawa (0.00 mg / kg), Kyambo (0.02 mg / kg), Dangyati Centres / points de collecte laitière (0.02 mg / kg) et Andaza (0.02 mg / kg). Nos résultats suggèrent que les niveaux de contamination au plomb détectés dans la zone d'étude sont cliniquement dangereux et insalubres pour la consommation humaine et ont de graves conséquences pour la santé publique. Par conséquent, des mesures urgentes doivent être prises par l'agence gouvernementale appropriée pour faire face à cette menace pour la santé vis-à-vis la sécurité des consommateurs des produits laitiers dans la zone d'étude.

show abstract

Section: Laboratory Analyses Of Heavy Metals Using H I G H P E R F O mentioning

confidence: 99%

Section: Laboratory Analyses Of Heavy Metals Using H I G H P E R F O mentioning

confidence: 99%

Section: Laboratory Analyses Of Heavy Metals Using H I G H P E R F O mentioning

confidence: 99%

Section: Laboratory Analyses Of Heavy Metals Using H I G H P E R F O mentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of lead and cobalt residues in locally processed yoghurt from native dairy cattle raised under traditional system of management

Onasanya¹,

Umar²,

Umar³

et al. 2020

NJAP

View full text Add to dashboard Cite

show abstract

“…Serum analysis by high-performance liquid chromatography.-All chemicals were reagent grade unless otherwise specified. McIlvaine buffer (Oka et al 1984) was prepared by dissolving 12.9 g of anhydrous citric acid (Mallinckrodt Baker, Phillipsburg, New Jersey) and 10.9 g of dibasic sodium phosphate (Fisher Scientific, Fair Lawn, New Jersey) in 1 L of water. McIlvaine-EDTA buffer was prepared by dissolving 37.2 g of EDTA disodium salt dihydrate (Sigma-Aldrich, St. Louis, Missouri) in up to 1 L of McIlvaine buffer with gentle heating.…”

Section: Experiments Day Actionmentioning

confidence: 99%

Oxytetracycline Pharmacokinetics in Rainbow Trout during and after an Orally Administered Medicated Feed Regimen

Miller

Pelsor

Kane³

et al. 2012

J Aqua Anim Hlth

View full text Add to dashboard Cite

The pharmacokinetic-pharmacodynamic predictor of antimicrobial activity for tetracyclines is reported to be the area under the concentration-time curve at steady state (AUC(ss)) divided by the minimal inhibitory concentration of the targeted pathogen. Here, we estimate AUC(ss) values for oxytetracycline (OTC) in serum of rainbow trout Oncorhynchus mykiss by using a destructive sampling study design. Seventy-two rainbow trout were fed OTC-medicated feed at 74.7 +/- 1.5 mg/kg (mean +/- SD) body weight (BW) by oral gavage for 10 consecutive days. Serum was collected from nine fish at 1, 3, 6, 8, 10, 12, 15, and 22 d after dosing began. Serum OTC concentrations were measured by high-performance liquid chromatography with a 0.01-microg/mL limit of detection. The average OTC AUC(ss) was 29.2 microg x h/mL and was estimated using nonlinear mixed-effects modeling and bootstrap resampling techniques. The elimination half-life was estimated as 85.0 h, and the fraction of steady state achieved was estimated as 0.85. The calculated AUC(ss) (24.8 microg x h/mL) following 10 d of oral dosing with 75 mg OTC/kg BW was less than the estimated AUC(ss). Results suggest that the pharmacokinetics of OTC exposure, including the AUC(ss), is better evaluated by using multiday dosimetry than by using a standard single-dose protocol.

show abstract

Normal silica gel and reversed phase thin-layer chromatography coupled with UV spectroscopy and IR-MALDI-o-TOF-MS for the detection of tetracycline antibiotics

et al. 2010

View full text Add to dashboard Cite

Tetracyclines (TCs) form a group of bacteriostatic antibiotics with closely related structures and similar chemical and physicochemical properties. They are widely employed as therapeutics in human and veterinary medicine. Here, we introduce the combination of UV spectroscopic detection of high-performance thin-layer chromatography (HPTLC)-separated TCs with direct analysis on solid phase using infrared matrix-assisted laser desorption/ionization orthogonal time-of-flight mass spectrometry (IR-MALDI-o-TOF-MS). Normal silica gel phase- and water-wettable hybrid C(18) reversed phase layers (RP-18W) both allowed HPTLC separation and sensitive UV spectroscopic detection followed by MS analysis of distinct TCs bands using the liquid matrix glycerol. The novel approach of direct IR-MALDI-o-TOF-MS analysis resulted in the unequivocal identification of four structurally different TCs employed in this study, and linear calibration curves were produced for analyte amounts from 20 ng to 1 μg. MS analysis of TCs from RP-18W HPTLC plates was found to be superior when compared to the spectra acquired from the silica gel layer. Ionic analytes obtained from the RP-18W surface are mainly detected as protonated species of high abundance accompanied by a reduced formation of adducts as well as background ions arising from the matrix or the stationary phase. This results in decreased complexity of the spectra and enhanced sensitivity of the combinatorial method. An approximate detection limit of 5 ng of individual TCs in mixtures by combining RP-18W HPTLC with IR-MALDI-o-TOF-MS offers a novel timely and cost-efficient method for tracing TCs.

show abstract

Improvement of the chemical analysis of antibiotics I. Simple method for the analysis of tetracyclines on silica gel high-performance thin-layer plates

Cited by 22 publications

References 21 publications

Evaluation of lead and cobalt residues in locally processed yoghurt from native dairy cattle raised under traditional system of management

Evaluation of lead and cobalt residues in locally processed yoghurt from native dairy cattle raised under traditional system of management

Oxytetracycline Pharmacokinetics in Rainbow Trout during and after an Orally Administered Medicated Feed Regimen

Normal silica gel and reversed phase thin-layer chromatography coupled with UV spectroscopy and IR-MALDI-o-TOF-MS for the detection of tetracycline antibiotics

Contact Info

Product

Resources

About