Separation and quantitative analysis of alkyl sulfobetaine-type detergents by high-performance liquid chromatography and light-scattering detection

Molecular Dynamics Simulations of Specific Anion Adsorption on Sulfobetaine (SB3-14) Micelles

“…In fact, a detailed structural model and a microscopic understanding of these systems are important to explain their physical and chemical properties, which requires detailed information about the interactions of the ions with micelles and solvent as well as the equilibrium structures and fluctuations (dynamics) of the micelle–ion systems, in addition to many-body effects. The properties of these systems have been probed by several experimental techniques, such as NMR, − radiation scattering, − zeta-potential, , neutron diffraction, , electrophoresis, , vibrational, , and time-resolved fluorescence spectroscopies. , Even with this wide variety of experimental methods used to probe micelle–ion systems, it is still very difficult to obtain detailed structural data and information about surface phenomena at the molecular level. To this end, molecular simulations have become an important and efficient tool to describe the structure, fluctuation, and dynamics of micelles. − Molecular dynamics (MD) methods using atomistic or coarse-grained potentials are the most commonly used approaches − because they provide results (structures, distributions, diffusion, viscosity, etc.)…”

Optimization and correlation of HPLC-ELSD and HPLC–MS/MS methods for identification and characterization of sophorolipids

“…A possible alternative could be a refractive index detector but the incompatibility with gradient elution and the lack of sensitivity makes the analysis extremely difficult [32]. These problems can be overcome, selecting an evaporative light scattering detector (ELSD).…”

Igni18, a novel metallo-hydrolase from the hyperthermophilic archaeon Ignicoccus hospitalis KIN4/I: cloning, expression, purification and X-ray analysis

“…For immobilized metal ion-affinity chromatography (IMAC) purification of Igni18_Myc_His 6 , the cells were thawed on ice and suspended in 5 ml lysis buffer [10 mg ml À1 myristyl sulfobetaine (SB3-14), 1 mM phenylmethylsulfonyl fluoride (PMSF), 0. incubating the cells at 343 K for 1 h in the presence of the zwitterionic detergent SB3-14 (Zanna & Haeuw, 2007). Cell debris was removed from the crude cell extract by centrifugation at 15 000 rev min À1 for 30 min at 277 K (Sorvall RC6+ centrifuge, SS-34 rotor; Thermo Scientific, Braunschweig, Germany).…”