Optimization and correlation of HPLC-ELSD and HPLC–MS/MS methods for identification and characterization of sophorolipids

Ribeiro, Isabel; Bronze, Maria Rosário; Castro, Matilde; Ribeiro, Maria H.L.

doi:10.1016/j.jchromb.2012.04.037

Cited by 42 publications

(40 citation statements)

References 37 publications

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“…Band 4 with the maximum R f of 0.83 represents L‐SLs. Ribeiro et al reported A‐ SLs on the TLC plate showed small R f values in the range of 0.18–0.41, which was consistent with our data. The results proved that more L‐SLs were obtained in the Mg 2+ condition than that in Fe 2+ condition.…”

Section: Resultssupporting

confidence: 93%

“…The MS results showed that the peak at retention time 21.26 min occurred at m/z 724.4 and 729.5, corresponding to [M + NH 4 ] + and [M + Na] + ; the peak at retention time 34.30 min occurred at m/z 687.6, 704.6, and 709.5, corresponding to [M + H] + , [M + NH 4 ] + , and [M + Na] + ; the peak at retention time 38.66 min occurred at m/z 689.5, 706.6, and 711.5, corresponding to [M + H] + , [M + NH 4 ] + , and [M + Na] + (Fig. ).The mass to charge ratio (m/z) in the spectra showed that the molecular weight of SL‐1, SL‐5, and SL‐7 was 706, 686, and 688, which revealed the types as A–SLs–C18:1 diacetylated, L–SLs–C18:2 diacetylated, and L–SLs–C18:1 diacetylated . The A‐C18:0 diacetylated samples show retention times of 22.04 and 23.12 min probably due to their difference between omega and omega‐1 linkages.…”

Section: Resultsmentioning

confidence: 99%

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Metal ions can affect the composition and production of sophorolipids by Wickerhamiella domercqiae Y_2A CGMCC 3798

Chen

Zhang

Liu

et al. 2014

Euro J Lipid Sci & Tech

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Different metal ions were added as supplements to culture media to study sophorolipids production by Wickerhamiella domercqiae Y 2A CGMCC 3798. The fermentation media were supplemented with different metal ions in the form of sulfate. A maximum yield 149.90 g/L was achieved in the presence of Cu 2þ ions. The production of lactonic sophorolipids was favored when culture media wad supplied with Mg 2þ ions, while Fe 2þ ions were the optimal ions for acidic sophorolipids production. TLC, HPLC, and LC-MS analyses were used for further investigation on the sophorolipids production. The results of SDS-PAGE and enzymatic transformation of acidic sophorolipids showed differential expression of extracellular proteins of W. domercqiae Y 2A CGMCC 3798 in presence of Mg 2þ or Fe 2þ . Overall the results indicated potential selective production of different sophorolipids based on selected metal ions in culture media.Practical applications: During the fermentation process of sophorolipids, L-SLs and A-SLs were produced selectively by adding Mg 2þ and Fe 2þ into fermentation media, respectively.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Metal ions can affect the composition and production of sophorolipids by Wickerhamiella domercqiae Y_2A CGMCC 3798

Chen

Zhang

Liu

et al. 2014

Euro J Lipid Sci & Tech

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“…The mass spectral ions were identified by calculation of elemental composition and comparison with available literature [35, 36]. The purified biosurfactant sample was a mixture of five components.…”

Section: Resultsmentioning

confidence: 99%

Production, characterization, and antifungal activity of a biosurfactant produced by Rhodotorula babjevae YS3

et al. 2017

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BackgroundSophorolipids are one of the most promising glycolipid biosurfactants and have been successfully employed in bioremediation and various other industrial sectors. They have also been described to exhibit antimicrobial activity against different bacterial species. Nevertheless, previous literature pertaining to the antifungal activity of sophorolipids are limited indicating the need for further research to explore novel strains with wide antimicrobial activity. A novel yeast strain, Rhodotorula babjevae YS3, was recently isolated from an agricultural field in Assam, Northeast India. This study was primarily emphasized at the characterization and subsequent evaluation of antifungal activity of the sophorolipid biosurfactant produced by R. babjevae YS3.ResultsThe growth kinetics and biosurfactant production by R. babjevae YS3 was evaluated by cultivation in Bushnell-Haas medium containing glucose (10% w/v) as the sole carbon source. A reduction in the surface tension of the culture medium from 70 to 32.6 mN/m was observed after 24 h. The yield of crude biosurfactant was recorded to be 19.0 g/l which might further increase after optimization of the growth parameters. The biosurfactant was characterized to be a heterogeneous sophorolipid (SL) with both lactonic and acidic forms after TLC, FTIR and LC–MS analyses. The SL exhibited excellent oil spreading and emulsifying activity against crude oil at 38.46 mm2 and 100% respectively. The CMC was observed to be 130 mg/l. The stability of the SL was evaluated over a wide range of pH (2–10), salinity (2–10% NaCl) and temperature (at 120 °C for time intervals of 30 up to 120 min). The SL was found to retain surface-active properties under the extreme conditions. Additionally, the SL exhibited promising antifungal activity against a considerably broad group of pathogenic fungi viz. Colletotrichum gloeosporioides, Fusarium verticilliodes, Fusarium oxysporum f. sp. pisi, Corynespora cassiicola, and Trichophyton rubrum.ConclusionsThe study reports, for the first time, the biosurfactant producing ability of R. babjevae, a relatively lesser studied yeast. The persistent surface active properties of the sophorolipid in extreme conditions advocates its applicability in diverse environmental and industrial sectors. Further, antifungal activities against plant and human pathogens opens up possibilities for development of efficient and eco-friendly antifungal agents with agricultural and biomedical applications.

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“…Although another species of Rhodotorula, i.e., R. bogoriensis has been well-documented as a SL producer (Nuñez et al, 2004;Ribeiro et al, 2012), R. babjevae has been described as a biosurfactant producer for the very first time in our previous report (Sen et al, 2017). However, a couple of studies involving R. babjevae strains UCDFST 04-877 (Cajka et al, 2016) and Y-SL7 (Guerfali et al, 2019) described the biosurfactant as polyol esters of fatty acids (PEFA).…”

Section: Discussionmentioning

confidence: 99%

Sophorolipid Biosurfactant Can Control Cutaneous Dermatophytosis Caused by Trichophyton mentagrophytes

et al. 2020

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Optimization and correlation of HPLC-ELSD and HPLC–MS/MS methods for identification and characterization of sophorolipids

Cited by 42 publications

References 37 publications

Metal ions can affect the composition and production of sophorolipids by Wickerhamiella domercqiae Y_2A CGMCC 3798

Metal ions can affect the composition and production of sophorolipids by Wickerhamiella domercqiae Y_2A CGMCC 3798

Production, characterization, and antifungal activity of a biosurfactant produced by Rhodotorula babjevae YS3

Sophorolipid Biosurfactant Can Control Cutaneous Dermatophytosis Caused by Trichophyton mentagrophytes

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Optimization and correlation of HPLC-ELSD and HPLC–MS/MS methods for identification and characterization of sophorolipids

Cited by 42 publications

References 37 publications

Metal ions can affect the composition and production of sophorolipids by Wickerhamiella domercqiae Y2A CGMCC 3798

Metal ions can affect the composition and production of sophorolipids by Wickerhamiella domercqiae Y2A CGMCC 3798

Production, characterization, and antifungal activity of a biosurfactant produced by Rhodotorula babjevae YS3

Sophorolipid Biosurfactant Can Control Cutaneous Dermatophytosis Caused by Trichophyton mentagrophytes

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Metal ions can affect the composition and production of sophorolipids by Wickerhamiella domercqiae Y_2A CGMCC 3798

Metal ions can affect the composition and production of sophorolipids by Wickerhamiella domercqiae Y_2A CGMCC 3798