Separation and purification of 10-deacetylbaccatin III by high-speed counter-current chromatography

Cao, Xueli; Tian, Yu; Zhang, Tian You; Ito, Yoichiro

doi:10.1016/s0021-9673(98)00360-4

Cited by 18 publications

(11 citation statements)

References 7 publications

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“…Taxoids have been analyzed by thin-layer chromatography (TLC) (Fujino et al, 2001), high-performance liquid chromatographyphotodiode array detection (HPLC-PAD) (Neméth-Klss et al, 1996), high-performance liquid chromatography-UV detection (HPLC-UV; Theodoridis et al, 1998), micellar electrokinetic capillary chromatography (MECC; Hempel et al, 1996) and high-speed counter-current chromatography (HSCCC; Cao et al, 1998). Short-term methods to determine taxoids have been described using HPLC-MS (Madhusudanan et al, 2002a,b;Kuppens et al, 2005;Mroczek et al, 2000;Mortier et al, 2004).…”

Section: Simultaneous Determination Of Main Taxoids Inmentioning

confidence: 99%

Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Sun

et al. 2008

Biomedical Chromatography

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A simple and accurate RP-HPLC method with pentafluorophenyl (PFP) column was developed for the simultaneous determination of six taxoids, i.e. paclitaxel, 10-deacetylbaccatin III (10-DAB III), 7-xylosyl-10-deacetyltaxol (7-xyl-10-DAT), 10-deacetyltaxol (10-DAT), cephalomannine and 7-epi-10-deacetyltaxol (7-epi-10-DAT), in the extracts from the needles of three Taxus species. The mobile phase consisted of acetonitrile (A) and water (B), and the extracts were separated using gradient elution program: 30% A at the first 7 min, and then ramped to 42% A at 8 min, held until 38 min. The developed method was validated with satisfactory precision (RSD < 2.61%), repeatability (RSD < 2.92%) and recovery (95.19-104.47%). The above taxoids in the extracts of Taxus cuspidata, T. chinensis and T. media were analyzed with the developed RP-HPLC method, and the results showed that the contents of different taxoids in three mentioned species were distinct. Maximal amounts of 10-DAB III, 7-xyl-10-DAT and 7-epi-10-DAT appeared in T. chinensis, while T. media possessed the highest content of 10-DAT, cephalomannine and paclitaxel. The developed method is accurate and efficient. It can be reliably used in the improved determination of taxoids for the quality control of Taxus species.

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Section: Simultaneous Determination Of Main Taxoids Inmentioning

confidence: 99%

Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Sun

et al. 2008

Biomedical Chromatography

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“…10-DAB III is also used for synthesizing docetaxel (Taxotere, an analog of paclitaxel) that possesses an improved pharmaceutical property. Consequently, considerable interest exists in the isolation of this compound [11,12]. There are also reports on other taxoids that can be applied in semisynthesis such as baccatin III and cephalomannine [9,13].…”

Section: Introductionmentioning

confidence: 99%

“…So far, taxoids have been analyzed by TLC [17], GC, HPLC [18,19], immunoaffinity chromatography [20], highspeed countercurrent chromatography (HSCCC) [11], and MEKC [21]. And with the development of analytical methods, rapid taxoids determination by HPLC (photodiode array detector (PAD))-MS appeared [22 -26], which made the analytical time shorter and increased the veracity of analysis also.…”

Section: Introductionmentioning

confidence: 99%

Rapid separation of four main taxoids in Taxus species by a combined LLP‐SPE‐HPLC (PAD) procedure

Fu²,

et al. 2006

J of Separation Science

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A method is described for the simultaneous determination of paclitaxel and three related taxoids, 10-deacetylbaccatin III (10-DAB III), baccatin III, and cephalomannine, in the extracts from the needles of three Chinese yew species, Taxus cuspidata, T. chinensis, and T. media. SPE was applied as the sample preparation technique and RP-HPLC with a photodiode array detector (PAD) was used for the analysis of extract samples. The crude extracts were treated with an improved SPE cartridge packed with a combination of 1-vinyl-pyrrolidin-2-one and divinyl-benzene. The eluent was 75% methanol. The following separation was achieved with a gradient program on an HIQ SIL C18W column in a system of ACN/water within 60 min. The samples were detected by PAD at wavelengths of 232.1 nm for 10-DAB III, 229.8 nm for baccatin III and paclitaxel, and 223.9 nm for cephalomannine. The content of 10-DAB III, baccatin III, cephalomannine, and paclitaxel varied from 0.0277 to 0.0875, 0.0254 to 0.0405, 0.0715 to 0.2486, and 0.0996 to 0.1301 mg/g in fresh needles of the above yew species, respectively. The assay achieved good resolution in the separation between the four compounds, and it can be used for quality control or purity determination for those in bulk and pharmceutical dosage forms.

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“…The reported methods, however, had some drawbacks. TLC [28,29], MECC [30,31] and high-speed counter-current chromatography (HSCCC) [32,33] all can separate taxoids, but they cannot adapt to mass production. Chemical reac-tion method was used to separate taxoids with the transformed structures [34,35], but it needs strict reaction condition and is difficult to control.…”

Section: Introductionmentioning

confidence: 99%

Preparative separation and enrichment of four taxoids from Taxus chinensis needles extracts by macroporous resin column chromatography

Sun

et al. 2009

J of Separation Science

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Preparative separation and enrichment of four taxoids from Taxus chinensis needles extracts by macroporous resin column chromatography An environment-friendly method was established for the preparative separation and enrichment of four taxoids, namely 10-deacetylbaccatin III (10-DAB III), 7-xylosyl-10-deacetyltaxol (7-xyl-10-DAT), cephalomannine and paclitaxel from Taxus chinensis needles extracts. Characteristics of seven widely used macroporous resins for four taxoids were compared, AB-8 resin offered better adsorption and desorption capacities than others. AB-8 resin column chromatography was used to study the desorption process for four taxoids. The optimum parameters for desorption were 30% ethanol 5 RV for removing impurities, following 15 RV for 10-DAB III, after the desorption of impurities with 35% ethanol 10 RV, 45% ethanol 30 RV for 7-xyl-10-DAT, then 65% ethanol 10 RV for cephalomannine and paclitaxel, the flow rate was 6 RV/ h. After separation on AB-8 resin column chromatography, the contents of 10-DAB III, 7-xyl-10-DAT, cephalomannine and paclitaxel in the product reached 4.58, 13.17, 1.36 and 3.08%, respectively, which were 7.63-, 3.68-, 6.18-and 6.55-fold to those in T. chinensis needles extracts. The recovery yields were 94.96, 77.32, 88.09 and 95.25%. In general, the AB-8 resin column chromatography has the advantages of lower cost, high efficiency and simple procedure. Therefore, it may provide scientific references for the preparative separation and enrichment of taxoids from other T. species.

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Separation and purification of 10-deacetylbaccatin III by high-speed counter-current chromatography

Cited by 18 publications

References 7 publications

Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Rapid separation of four main taxoids in Taxus species by a combined LLP‐SPE‐HPLC (PAD) procedure

Preparative separation and enrichment of four taxoids from Taxus chinensis needles extracts by macroporous resin column chromatography

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