Preparative separation and enrichment of four taxoids from Taxus chinensis needles extracts by macroporous resin column chromatography

Abstract

Preparative separation and enrichment of four taxoids from Taxus chinensis needles extracts by macroporous resin column chromatography An environment-friendly method was established for the preparative separation and enrichment of four taxoids, namely 10-deacetylbaccatin III (10-DAB III), 7-xylosyl-10-deacetyltaxol (7-xyl-10-DAT), cephalomannine and paclitaxel from Taxus chinensis needles extracts. Characteristics of seven widely used macroporous resins for four taxoids were compared, AB-8 resin offered better adsorption and desorption capacities than others. AB-8 resin column chromatography was used to study the desorption process for four taxoids. The optimum parameters for desorption were 30% ethanol 5 RV for removing impurities, following 15 RV for 10-DAB III, after the desorption of impurities with 35% ethanol 10 RV, 45% ethanol 30 RV for 7-xyl-10-DAT, then 65% ethanol 10 RV for cephalomannine and paclitaxel, the flow rate was 6 RV/ h. After separation on AB-8 resin column chromatography, the contents of 10-DAB III, 7-xyl-10-DAT, cephalomannine and paclitaxel in the product reached 4.58, 13.17, 1.36 and 3.08%, respectively, which were 7.63-, 3.68-, 6.18-and 6.55-fold to those in T. chinensis needles extracts. The recovery yields were 94.96, 77.32, 88.09 and 95.25%. In general, the AB-8 resin column chromatography has the advantages of lower cost, high efficiency and simple procedure. Therefore, it may provide scientific references for the preparative separation and enrichment of taxoids from other T. species.

Effects of the processing steps on parathion levels during honey production and parathion removal by macroporous adsorption resins

“…Macroporous adsorption resins (MARs) are often used to remove OPs in industry due to its less yielding cost, simpler operation, higher efficiency, more friendly to environmental protection and easier regeneration (Du, Yuan, Li, & Zhou, 2008;Fu et al, 2006;Ma et al, 2009;Sun et al, 2009). Fig.…”

Preparation and characterization of molecular imprinted polymer functionalized with core/shell magnetic particles (Fe 3 O 4 @SiO 2 @MIP) for the simultaneous recognition and enrichment of four taxoids in Taxus × media

“…standard Gibbs free energy change (∆G o , (kJ mol -1 ), standard enthalpy change (∆H o , kJ mol -1 ) and standard entropy change (∆S o , kJ mol -1 K -1 ) for the adsorption of paclitaxel on Fe 3 O 4 @SiO 2 @MIP and Fe 3 O 4 @SiO 2 @NMIP were calculated by Eqs. (10) and (11) [17,36]. 15 Where K (L mol -1 ) is the constant of Langmuir equation, R (8.314 J mol -1 K -1 ) is the gas constant, and T (K) represents the absolute temperature.…”

“…Moreover, 10-DAB and baccatin III can be transferred to paclitaxel or its derivatives through the biosynthesis or chemosynthesis methods [5][6][7]. Therefore, all of these four taxoids were so valuable that various sorbents were used to separate or enrich them, such as silica [8], macroporous resins [9][10], and reverse phase silica C18 [11], but these sorbents inherently lacked selectivity. Therefore, a novel sorbent should be developed for selective and simultaneous separation of these four taxoids in order to completely utilize the bioresource of T. ×…”

Adsorption behaviors for oleuropein from olive leaves extracts by porous materials with carbon nanotubes

“…To obtain active components with high concentration, many techniques [7][8][9] like extraction, and high-speed counter-current chromatography, were involved in the separation of active components from natural plant resources. Howbeit, the methods mentioned above exhibited several limits, such as time consuming, harmful to laborers, environmental pollution, and consumption of bulk amount of solvents.…”