1966
DOI: 10.1128/jb.91.6.2200-2204.1966
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Separation and Properties of a Red Cell Sensitizing Substance from Streptococci

Abstract: Separation and properties of a red cell sensitizing substance from streptococci. J. Bacteriol. 91:2200-2204. 1966.-An antigen that binds onto red cells and causes them to be agglutinated by antiserum was separated from streptococci. Various procedures to extract the antigen from streptococci were investigated, and the greatest amount of antigen was obtained by extraction of cells with a phenol-water mixture. The reaction of the antigen with red cells was shown to be reversible by use of the Ashby mixed aggluti… Show more

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Cited by 80 publications
(27 citation statements)
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“…), and washed three times in distilled water . LTA was extracted by the method of Moskowitz (14) with minor modifications . 5 g (wet-packed weight) of washed streptococci were resuspended in 50 ml of 0.2 N HCl and heated in a boiling (95°C) water bath for 10 min .…”
Section: Methodsmentioning
confidence: 99%
“…), and washed three times in distilled water . LTA was extracted by the method of Moskowitz (14) with minor modifications . 5 g (wet-packed weight) of washed streptococci were resuspended in 50 ml of 0.2 N HCl and heated in a boiling (95°C) water bath for 10 min .…”
Section: Methodsmentioning
confidence: 99%
“…Cells were eelletted by centrifugation (I0 000 X g, 15 min), washed three times with water and lyophilized. Cellular antigens were prepared by phenol-water extraction according to the procedure of Moskowitz [13 ]. Whole cell lysate with mutanolysin [14] was also employed as a cellular antigen.…”
Section: Cultural Condition and Preparation Of Antigensmentioning
confidence: 99%
“…The amphipathic antigen of E. nodatum lost its binding ability for SRBC membrane on deacylation. The hydrophobic lipid portion of this amphipathic antigen appears to be essential for the eukaryotic cell binding, such as the LTA of group A streptococci [1,7].…”
Section: Resultsmentioning
confidence: 99%
“…Amphipathic antigen was extracted with phenol water from lyophilised whole cells according to the method of Moskowitz [7].…”
Section: Preparation Of the Amphipathic Antigenmentioning
confidence: 99%