Separation and estimation of tocopherols in vegetable oils by thin‐layer chromatography

Rao, M. K. Govind; Rao, S. Venkob; Achaya, K. T.

doi:10.1002/jsfa.2740160301

Cited by 23 publications

(4 citation statements)

References 16 publications

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“…The isomeric ß-and -tocopherols have been separated by thin-layer chromatography (Stowe, 1963). This method gave recoveries of 97-98%, with a synthetic mixture of -, ß-, -, and -tocopherols (Govind Rao et al, 1965). Two-dimensional thin-layer chromatography was attempted by Pennock et al (1964) to separate completely seven out of the eight known tocopherols and trienols, but their solvent system could not separate /3-tocotrienols from -tocopherol.…”

Section: T H Imentioning

confidence: 99%

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Identification and estimation of tocopherols and tocotrienols in vegetable oils using gas chromatography-mass spectrometry

Mk¹,

Eg²

1972

J. Agric. Food Chem.

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GOVIND RAO, PERKINS a fructosan-accumulating species (Smith and Grotelueschen, 1966), was used to evaluate this method for characterizing and quantitizing carbohydrate fractions in forage plants. Data indicated that roots of this species have a relatively high free fructose content. The increasing fructose/glucose (Cf/ Cg) ratio corresponding with decreasing ethanol extractions beginning with the 92.5 % hydrolyzed extraction indicated the presence of fructosans of varying chain lengths and the greatest quantity of fructose was extracted with 60% ethanol. These data corroborate the findings of Smith and Grotelueschen (1966). Fructosans in grasses are reported to be similar to the inulin type found in Compositae, but are thought to occur in chain lengths shorter than 35 fructofuranose residues linked 2:6 and terminated by a sucrose residue (Bacon, 1960; Edleman, 1960). In this experiment, fructose/glucose ratios increased with decreasing ethanol concentration indicating the presence of fructosans with average chain lengths of 1.05 (sucrose) at 92.5% ethanol extraction to 20.6 (inulin) at 0% ethanol extraction, in tall fescue roots.Colorimetric determination of fructose in the presence of glucose can be used advantageously for differentiating these two sugars in plant extracts where they are present in greatest quantities. This method, in combination with the ethanol dilution extractions and acid hydrolyses, allows for routine quantitative description of carbohydrates in plant material, with the exception of starch. Because of the simplicity of this method, it can be used for routine determination of sugar components in forage samples, in particular the fructosan accumulating species. LITERATURE CITED

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Section: T H Imentioning

confidence: 99%

“…These samples were accurately weighed and dissolved in benzene so as to give 1 mg/ml concentration. An artificial mixture of these compounds was made and their purity was calculated after their separation on thin-layer chromatography (Govind Rao et al, 1965).…”

Section: T H Imentioning

confidence: 99%

Identification and estimation of tocopherols and tocotrienols in vegetable oils using gas chromatography-mass spectrometry

Mk¹,

Eg²

1972

J. Agric. Food Chem.

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“…TLC on Silica gel G has been used for the assay of tocopherol in a wide variety of materials such as vegetable oils (20,24,25,29), leaves (18,19), alfalfa (21), blood serum ( 15,31,32), liver (17), and tocopherol oxidation products (16). In the case of blood serum, Horwitt et al (32) reported that the losses of tocopherol were excessive when blood serum containing less than 0.6 mg/100 ml was assayed by TLC.…”

Section: Thin Layermentioning

confidence: 99%

Modern procedures for the analysis of tocopherols

Bunnell

1971

Lipids

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The development of reliable assay methodology for the tocopherols has been an evolutionary process which has required over 40 years to reach its present state. Today the analyst has at his disposal a choice of reliable and accurate methods of analysis for the eight tocopherols now known to exist in nature. The general sequence of procedure and the precautions to be observed are described. After suitable extraction procedures and careful saponification techniques, the tocopherols in the nonsaponifiable fraction can be assayed by a variety of chromatographic procedures including paper, thin layer (TLC), column and gas liquid chromatography (GLC). The current methodology of these procedures is reviewed in some detail with special emphasis on the more frequently used TLC and GLC techniques. The earlier methods of GLC assay separated only the mono‐, di‐ and trimethylated tocopherols but methods are now available which provide separation of all the tocopherols. These developments were made possible by better columns and the use of derivatives of tocopherols. In addition to good separation, the GLC method is the most sensitive available for the quantitation of the tocopherols. Spectrophotometric measurements based on Emmerie Engel type reactions have been most frequently used for the final quantitative analysis of the tocopherols separated by paper, TLC, or column chromatography.

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“…8-Tocopherol, found originally in soya-bean oil at a level of 30% of the total tocopherols present, was soon found to be present in peanut and cottonseed oils . Since that time 8-tocopherol has been described in castor, neem, safflower, sesame, mustard and corn oils (Herting & Drury, 1963;Rao, Rao & Achaya, 1965), in raspberry and blackberry fruits (Booth & Bradford, 1963), in plant leaves (Booth, 1962) and in some seaweeds (Brown, 1953).…”

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The isolation and properties of δ-tocotrienol from Hevea latex

Whittle¹,

Dunphy²,

Jf³

1966

Biochemical Journal

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1. delta-Tocotrienol (8-methyltocotrienol) was isolated from the latex of Hevea brasiliensis. This new member of the tocopherol family is a pale-yellow oil at room temperature. 2. The properties of delta-tocotrienol are very similar to those of delta-tocopherol and the small differences can be explained by the change in side chain. 3. The ultraviolet and infrared spectra of delta-tocotrienol were determined and a conversion factor for use with the Emmerie-Engel reaction was worked out. Details are given for the chromatography of delta-tocotrienol on thin layers (adsorption and partition) and reversed-phase paper, and the nitroso derivatives were formed. 4. An ethyl carbonate ester of delta-tocotrienol was prepared and compared with a similar ester of delta-tocopherol. 5. Hydroxymethylation of delta-tocotrienol followed by reduction gave beta-tocotrienol as a major product.

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Separation and estimation of tocopherols in vegetable oils by thin‐layer chromatography

Cited by 23 publications

References 16 publications

Identification and estimation of tocopherols and tocotrienols in vegetable oils using gas chromatography-mass spectrometry

Identification and estimation of tocopherols and tocotrienols in vegetable oils using gas chromatography-mass spectrometry

Modern procedures for the analysis of tocopherols

The isolation and properties of δ-tocotrienol from Hevea latex

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