Separation and Determination of Iron-Containing Proteins via Liquid Chromatography−Particle Beam/Hollow Cathode−Optical Emission Spectroscopy

Brewer, Tim M.; Marcus, R. Kenneth

doi:10.1021/ac061516p

Cited by 12 publications

(26 citation statements)

References 57 publications

(135 reference statements)

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“…The HC glow discharge design was altered slightly from the one employed in previous works [10][11][12][13][15][16][17][18][19]21,22]. The HC was machined from a bulk copper rod with an inner diameter of 3.5 mm Fig.…”

Section: Hollow Cathode Glow Discharge Sourcementioning

confidence: 99%

“…The optimal parameters for the discharge conditions were set at a pressure of 2.0 Torr He and a discharge current of 60 mA. Past operating conditions for the PB/HC-OES system varied from 1.0-2.0 Torr and 40-60 mA depending on the type of analyte being examined [11,12,14,29], but the general trend is the same as past experiments in this laboratory [30].…”

Section: Conditions Effecting Analyte Responsementioning

confidence: 99%

“…It has been previously shown that vaporization from the HC walls is the primary means of generating gas-phase species in the PB/HC-OES system [16,19]. Optimal HC temperatures have ranged from 200-300°C, with higher temperatures leading to pyrolysis of carbonaceous samples [10,11,16,19,20]. Given the difference in the placement of the heaters and thermocouples in the new design, it was imperative to characterize the role of "measured" temperature on the analyte responses.…”

Section: Conditions Effecting Analyte Responsementioning

confidence: 99%

“…Techniques supplying both molecular and atomic information would be very powerful contributors in the field of metallomics. This laboratory has previously developed a liquid chromatography-particle beam/hollow cathode-optical emission spectrometer (LC-PB/HC-OES) that can analyze inorganic and/or organic samples, providing empirical formulas based on element ratios (including C, H, N, O, S, and metals), with detection limits on the single ng mL − 1 level and below [10][11][12][13]. The PB/HC-OES system combines the excitation properties of a glow discharge (GD) source with the solvent removal capabilities of the particle beam (PB) to allow dry analyte particles to reach the hollow cathode (HC) GD region [7].…”

Section: Introductionmentioning

confidence: 99%

“…Use of the PB allows work in an inert (discharge gas) atmosphere plasma, in the absence of solvent remnants, which makes it possible to detect "gaseous" elements and non-metals such as C, H, N, O, S, and P with high sensitivity. Previous experiments using monochromator detection system include determinations of metal salts and organometallic compounds, as well as the non-metal detection of proteins and amino acids based on C and H content [11][12][13][14][15][16][17][18]. Improvements have been made by substituting the monochromator with a polychromator, allowing for simultaneous, multielement detection, thus reducing data collection times, sample sizes, and the dependence on chromatographic retention times to substantiate elemental correlations.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Simultaneous multielement detection in particle beam/hollow cathode-optical emission spectroscopy

Quarles

Marcus

2009

Spectrochimica Acta Part B: Atomic Spectroscopy

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Section: Hollow Cathode Glow Discharge Sourcementioning

confidence: 99%

Section: Conditions Effecting Analyte Responsementioning

confidence: 99%

Section: Conditions Effecting Analyte Responsementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Simultaneous multielement detection in particle beam/hollow cathode-optical emission spectroscopy

Quarles

Marcus

2009

Spectrochimica Acta Part B: Atomic Spectroscopy

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Competitive binding of Fe3+, Cr3+, and Ni2+ to transferrin

2011

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Competitive binding of Fe(3+), Cr(3+), and Ni(2+) to transferrin (Tf) was investigated at various physiological iron to Tf concentration ratios. Loading percentages for these metal ions are based on a two M(n+) to one Tf (i.e., 100% loading) stoichiometry and were determined using a particle beam/hollow cathode-optical emission spectroscopy (PB/HC-OES) method. Serum iron concentrations typically found in normal, iron-deficient, iron-deficient from chronic disease, iron-deficient from inflammation, and iron-overload conditions were used to determine the effects of iron concentration on iron loading into Tf. The PB/HC-OES method allows the monitoring of metal ions in competition with Fe(3+) for Tf binding. Iron-overload concentrations impeded the ability of chromium (15.0 μM) or nickel (10.3 μM) to load completely into Tf. Low Fe(3+) uptake by Tf under iron-deficient or chronic disease iron concentrations limited Ni(2+) loading into Tf. Competitive binding kinetic studies were performed with Fe(3+), Cr(3+), and Ni(2+) to determine percentages of metal ion uptake into Tf as a function of time. The initial rates of Fe(3+) loading increased in the presence of nickel or chromium, with maximal Fe(3+) loading into Tf in all cases reaching approximately 24%. Addition of Cr(3+) to 50% preloaded Fe(3+)-Tf showed that excess chromium (15.0 μM) displaced roughly 13% of Fe(3+) from Tf, resulting in 7.6 ± 1.3% Cr(3+) loading of Tf. The PB/HC-OES method provides the ability to monitor multiple metal ions competing for Tf binding and will help to understand metal competition for Tf binding.

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Gene expression analysis of metallothionein and mineral elements uptake in tomato (Solanum lycopersicum) exposed to cadmium

2016

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Heavy metals such as Cd are considered to be the most important pollutants in soil contamination. Cd is a non-essential element adversely affecting plant growth and development, and it has caused some physiological and molecular changes. Metallothioneins (MTs) are low molecular weight, cysteine-rich, and metal binding proteins. In this study, we aimed to evaluate the MT gene expression levels and minerals uptake in the tissues of Solanum lycopersicum exposed to Cd. The transcriptional expression of the MT genes was determined by real-time quantitative PCR. The MT genes were regulated by the Cd and the mineral elements uptake changed tissue type and applied doses. The MT1 and MT2 transcript levels increased in the roots, the leaves and the fruits of the tomato. The MT3 and MT4 transcript pattern changed according to the tissue types. The Cd treatment on the growth medium increased the Mg, Ca, and Fe content in both the leaves and fruits of the tomato. However, the Cd affected the mineral levels in the roots depending on the mineral types and doses. Also, the Cd content increased in the roots, the leaves, and the fruits of the tomato, respectively. The results presented in this study show that Cd has synergistic and/or antagonistic effects on minerals depending on the tissue types. These results indicate that the MT1 and MT2 expression pattern increased together with the Mg, Ca, and Fe content in both the leaves and the fruits of the tomato.

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Separation and Determination of Iron-Containing Proteins via Liquid Chromatography−Particle Beam/Hollow Cathode−Optical Emission Spectroscopy

Cited by 12 publications

References 57 publications

Simultaneous multielement detection in particle beam/hollow cathode-optical emission spectroscopy

Simultaneous multielement detection in particle beam/hollow cathode-optical emission spectroscopy

Competitive binding of Fe3+, Cr3+, and Ni2+ to transferrin

Gene expression analysis of metallothionein and mineral elements uptake in tomato (Solanum lycopersicum) exposed to cadmium

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