Simultaneous multielement detection in particle beam/hollow cathode-optical emission spectroscopy

Quarles, C. Derrick; Marcus, R. Kenneth

doi:10.1016/j.sab.2009.08.004

Cited by 5 publications

(5 citation statements)

References 31 publications

(82 reference statements)

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“…7 The PB/HC-OES instrument is a home-built system that has been through 15 years of improvements and optimization. 7,[28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43] The particle beam consists of a thermoconcentric nebulizer, desolvation chamber, and a two stage momentum separator. The nebulizer creates an aerosol spray that enters the desolvation chamber where the particles are further desolvated before passing through a two stage momentum separator that removes all low mass particles (e.g.…”

Section: Pb/hc-oesmentioning

confidence: 99%

Instrumental comparison of the determination of Cr3+ uptake by human transferrin

2010

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UV-VIS absorbance, inductively coupled plasma-optical emission spectroscopy (ICP-OES), and particle beam/hollow cathode-optical emission spectroscopy (PB/HC-OES) are presented as techniques for determining Cr³+ loading into transferrin (Tf), with and without Fe³+. The methods are compared based on loading percentages (i.e. 100% loading would be equal to 2 M(n+): 1 Tf) determined for Cr³+ loading into apo-transferrin. Spectral interferences and overlapping LMCT bands cause inaccurate chromium (qualitative) and iron (qualitative and quantitative) results for the UV-VIS absorbance method. The ICP-OES and PB/HC-OES methods are in good agreement providing evidence that the PB/HC-OES method is a valid technique for investigating metal-protein complexes. Maximum Cr³+ loading into apo-transferrin over a 24 h period was determined to be 26.8 3.5% by the ICP-OES method and 25.3 2.2% by the PB/HC-OES method. Loading percentages were increased to 49.7 1.9% (ICP-OES) and 55.7 3.2% (PB/HC-OES) when the metal-transferrin solution was allowed to incubate for up to 10 days. Under non-excess carbonate conditions the Cr³+ loading is elevated over a 24 h incubation time, but under physiological conditions the loading is inhibited. Equal loading of Fe³+ and Cr³+ into apo-transferrin was achieved when chromium was at a level more than 5 times in excess of iron. Inhibition of Cr³+ loading was only observed when an excess of Fe³+ was available to bind into apo-transferrin. The ability for Cr³+ to displace Fe3+ from holo-transferrin was observed as small amounts of Cr³+ were loaded into the once occupied metal binding site.

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Section: Pb/hc-oesmentioning

confidence: 99%

Instrumental comparison of the determination of Cr3+ uptake by human transferrin

2010

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“…176 A comprehensive optimization of relevant operating conditions was made with nitrate salts of Ag, Ni, and Pb. The researchers found that monitoring multiple elements simultaneously revealed interelement matrix effects that had not been observed previously for hollow cathode sources.…”

Section: Detectionmentioning

confidence: 99%

“…The application of a particle beam/hollow cathode-optical emission spectrometer for simultaneous multi-element detection for chromatographic separations has been described. 176 A comprehensive optimization of relevant operating conditions was made with nitrate salts of Ag, Ni, and Pb. The researchers found that monitoring multiple elements simultaneously revealed interelement matrix effects that had not been observed previously for hollow cathode sources.…”

Section: Detectionmentioning

confidence: 99%

Atomic spectrometry update. Elemental speciation

Harrington

Clough

Drennan-Harris

et al. 2011

J. Anal. At. Spectrom.

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“…Several workers describe detectors with the aim of coupling with chromatography for speciation studies. Quarles and Marcus 174 have reported results of the development of a particle beam/hollow cathode AES device with a polychromator, to allow simultaneous multielement analysis. LODs for Pb, Ni, and Ag were 2.2, 0.17 and 0.19 ng respectively.…”

Section: Instrumentation and Fundamentals 21 Instrumentationmentioning

confidence: 99%