Separation and detection of gold nanoparticles with capillary electrophoresis and ICP-MS in single particle mode (CE-SP-ICP-MS)

Abstract

A method for the identification, separation, and detection of engineered nanoparticles using CE-ICP-MS in single particle mode is described.

Inductively coupled plasma mass spectrometry based platforms for studies involving nanoparticle effects in biological samples

“…In addition to those previously mentioned, other separation techniques coupled to ICP‐MS, such as capillary electrophoresis (CE), may also be used for the detection and evaluation of the effects of NPs in biological matrices . In addition to possessing high‐resolution separation capabilities, CE has been used for evaluating chemical reactions, and when coupled with ICP‐MS, it can provide multidimensional information regarding the separation and characterization of NPs, as well as their interactions with a given organism . One of the greatest concerns associated with NP evaluation using CE‐ICP‐MS is related to surfactant–particle interactions that may result in inconsistent migration behavior .…”

Single particle inductively coupled plasma mass spectrometry: investigating nonlinear response observed in pulse counting mode and extending the linear dynamic range by compensating for dead time related count losses on a microsecond timescale

“…A home-built prototype data acquisition system, specically tailored to the needs of spICP-MS, was used for acquisition of all data shown in the following. An earlier version of this protoDAQ was mentioned rst by the authors in 2016, 30 but was constantly improved and adapted to different use cases since then, 40 and a more advanced version was utilized recently in two studies performed by Mozhayeva et al 41,42 In its current version, the protoDAQ allows to acquire and transfer data to a host computer for virtually any measurement time at a sustained data rate of up to 2 Â 10 5 samples per second (i.e., with a highest time resolution of 5 ms) and with a duty cycle of 100% (no reset time between two subsequent data points). In this study, it captured the pulse counting signal directly behind the preamplier/discriminator stage of the instrument's detection electronics, providing an uninterrupted stream of data that represents the number of pulses observed in each dwell ("RAW data").…”

ICP-MS for the analysis at the nanoscale – a tutorial review

“…The CE buffer contained 70 mmol L -1 SDS and N-cyclohexyl-3-aminopropanesulfonic acid to achieve conditions for MEKC separation mode. 212 The developed method was applied for separation of the NIST Au nanoparticles of 10 nm, 30 nm and 60 nm (see Table 1). Baseline separation was not achieved, but in particular the separation of the 60 nm particles from the 30 nm particles was much better compared to RPC 201 , SEC 192 and HDC 192 .…”