Separation and Characterization of Antioxidative and Angiotensin Converting Enzyme Inhibitory Peptide from Jellyfish Gonad Hydrolysate

Abstract

The gonad of jellyfish (Rhopilema esculentum Kishinouye), containing high protein content with a rich amino acid composition, is suitable for the preparation of bioactive peptides. Jellyfish gonad was hydrolysed with neutral protease to obtain jellyfish gonad protein hydrolysate (JGPH), which was then purified sequentially by ultrafiltration, gel filtration chromatography, and RP-HPLC. The peptides were characterized with HPLC-MS/MS. One peptide with amino acid sequence Ser-Tyr (SY) was identified and synthesized, which showed good ACE inhibitory and antioxidant activity. The IC50 of this peptide on DPPH, ·OH, super oxygen anion scavenging activities, and ACE inhibitory activity are 84.623 μM, 1177.632 μM, 456.663 μM, and 1164.179 μM, respectively. The anchor in the binding site of SY and ACE C-domain (ACE-C) was obtained by molecular simulations. The results showed that the dipeptide purified from jellyfish gonad protein hydrolysates can be used as functional food material and is helpful in the study of antioxidant and inhibition of ACE.

Phenolic Profile, Antioxidant Capacities and Enzymatic Inhibitory Activities of Propolis from Different Geographical Areas: Needs for Analytical Harmonization

“…For this reason, it is of paramount importance to research other substances, especially natural products that could inhibit ACE to some extent. Most studies are focussed in peptides obtained from different food proteins, such as egg white [58], soybean [59], seafood [60,61] and other protein rich foods. Other studies show that some phenolic compounds, such as flavonoids, also exhibit ACE inhibition activity [62], so flavonoid extracts or products rich in flavonoids might be used against hypertension.…”

“…Other studies show that some phenolic compounds, such as flavonoids, also exhibit ACE inhibition activity [62], so flavonoid extracts or products rich in flavonoids might be used against hypertension. With regard to propolis, further studies are still necessary to research the ACE inhibitory action at various concentrations in order to check if this activity depends on propolis concentration, as it was described for other products [60,61]. It is also necessary to perform more in vivo studies, similar to the study investigating Brazilian green propolis for the prevention of hypertension in rats [63].…”

Mediterranean jellyfish as novel food: effects of thermal processing on antioxidant, phenolic, and protein contents

“…Several peptides from protein foods or ingredients, including seafood by-products [27] and jellyfish [12,13,[28][29][30][31][32][33][34][35] have been found to possess antioxidant capacity and they can contribute to the endogenous antioxidant capacity of foods. Proteins also have excellent potential as antioxidant additives in foods because they can inhibit lipid oxidation through multiple pathways including inactivation of reactive oxygen species, scavenging free radicals, chelation of prooxidative transition metals, reduction of hydroperoxides, and alteration of the physical properties of food systems [36,37].…”

Preparation, Identification, and Activity Evaluation of Eight Antioxidant Peptides from Protein Hydrolysate of Hairtail (Trichiurus japonicas) Muscle

“…More importantly, antioxidant peptides can exert their activity as free radical scavengers, peroxide decomposers, metal inactivators and oxygen inhibitors to protect food and organisms from ROS [11,12,13]. In recent years, antioxidant peptides derived from seafood were more attractive and have been isolated and identified from diverse marine organisms, such as swim bladders of miiuy croaker ( Miichthys miiuy ) [6], bluefin leatherjacket ( Navodon septentrionalis ) heads and skin [8,14], thornback ray skins [15], Palmaria palmate [16], viscera and carcass of Nile tilapia [17], Pinctada fucata [18], pectoral fin of salmon [19], and jellyfish gonad [20]. Zhao et al isolated ten antioxidant peptides from swim bladders of miiuy croaker, and PYLRH and GIEWA exhibited stronger scavenging activities on 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH•), hydroxyl radical (HO•), and superoxide anion radical (normalO2−•) than other eight peptides.…”