Separation and analysis of phenolic acids from  Salvia miltiorrhiza  and its related preparations by off-line two-dimensional hydrophilic interaction chromatography  ×  reversed-phase liquid chromatography coupled with ion trap time-of-flight mass spectrometry

Sun, Wanyang; Tong, Ling; Miao, Jingzhuo; Huang, Jingyi; Li, Dongxiang; Li, Yunfei; Xiao, Hongting; Sun, Henry; Bi, Kaishun

doi:10.1016/j.chroma.2015.12.038

Cited by 54 publications

(17 citation statements)

References 48 publications

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“…In our study, MS detection was performed in the negative ion mode, since this mode was more sensitive than the positive mode for phenolic acids (Sun et al ., ). The optimization of MS parameters was performed by infusing reference compounds solutions individually to achieve maximum response.…”

Section: Resultsmentioning

confidence: 97%

Characterization of metabolites in rats after intravenous administration of salvianolic acid for injection by ultra‐performance liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Miao

Sun

Huang

et al. 2016

Biomedical Chromatography

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It is an essential requirement to clarify the metabolites of traditional Chinese medicine (TCM) injections, which contain numerous ingredients, to assess their safe and effective use in clinic. Salvianolic acid for injection (SAFI), made from hydrophilic phenolic acids in Salvia miltiorrhiza Bunge, has been widely used for the treatment of cerebrovascular diseases, but information on its metabolites in vivo is still lacking. In the present study, we aimed to holistically characterize the metabolites of the main active ingredients in rat plasma, bile, urine and feces following intravenous administration of SAFI. An ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS) method was developed. Combining information on retention behaviors, multistage mass spectra and literature data, a total of eight prototypes and 52 metabolites were tentatively characterized. Metabolites originated from rosmarinic acid and salvianolic acid B comprised the majority of identified compounds. Meanwhile, four metabolites derived from salvianolic acid D and five from salvianolic acid B are reported for the first time. This study revealed that methylation, sulfation and glucuronidation were the major metabolic pathways of phenolic acids in SAFI in vivo. Furthermore, the developed UPLC/Q-TOF-MS method could also benefit the metabolic investigation of extracts and preparations in TCM with hydrophilic ingredients. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Resultsmentioning

confidence: 97%

Characterization of metabolites in rats after intravenous administration of salvianolic acid for injection by ultra‐performance liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Miao

Sun

Huang

et al. 2016

Biomedical Chromatography

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“…Salvia miltiorrhiza , the dry root and rhizome of Salvia miltiorrhiza Bge ., is one of the most commonly used traditional Chinese medicines for promoting blood circulation and removing blood stasis (Chen, Guo, Bao, Lu, & Wang, ; Xiong, ). Salvia miltiorrhiza polyphenolic acid (SMPA) is the main active component of Salvia miltiorrhiza , including salvianolic acid B (Sal B), rosmarinic acid (Ros A) and Danshensu (DA) (Sun et al, ). In addition to its protective effect on the cardiovascular system (Ho & Hong, ), it also plays an important role in the treatment of lung diseases such as idiopathic pulmonary fibrosis and chronic obstructive pulmonary disease (Huang et al, ).…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics of salvianolic acid B, rosmarinic acid and Danshensu in rat after pulmonary administration of Salvia miltiorrhiza polyphenolic acid solution

Lü

Xing

Xue

et al. 2019

Biomedical Chromatography

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A sensitive and accurate LC-MS/MS method was established for quantifying salvianolic acid B (Sal B), rosmarinic acid (Ros A) and Danshensu (DA) in rat plasma. Salvia miltiorrhiza polyphenolic acid (SMPA), active water-soluble ingredients isolated and purified from Salvia miltiorrhiza Bge included Sal B, Ros A and DA. The pharmacokinetic analysis of Sal B, Ros A and DA after pulmonary administration of SMPA solution to rat was performed by LC-MS/MS. Results from the pharmacokinetic studies showed that the peak concentration of DA was 21.85 ± 6.43 and 65.39 ± 3.83 ng/ mL after pulmonary and intravenous administration, respectively. DA was not detected at 2 h after administration. The absolute bioavailabilities of Sal B and Ros A were respectively 50.37 ± 27.04 and 89.63 ± 12.16% after pulmonary administration of 10 mg/kg SMPA solution in rats. The absolute bioavailability of Sal B increased at least 10-fold after pulmonary administration, compared with oral administration. It was concluded that the newly established LC-MS/MS method was suitable for describing the pharmacokinetic characteristics of Sal B, Ros A and DA in rat after pulmonary administration of SMPA solution. The data from this study will provide a preclinical insight into the feasibility of pulmonary administration of SMPA. KEYWORDS LC-MS/MS, pharmacokinetics, pulmonary administration, Salvia miltiorrhiza polyphenolic acid

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“…It is clear that full profiling of chemically complex samples using LC‐MS is rather difficult if not impossible . The emergence of comprehensive two‐dimensional LC (LC × LC) that combines two separation mechanisms has provided an alternative platform for high resolution analysis of complex real‐world samples . In comparison with conventional 1D‐LC, LC × LC provides higher resolving power (as a result of a multiplicative increase in peak capacity), in addition to structured chromatogram where components are spread out in 2D space according to chemical specificity of retention patterns .…”

Section: Introductionmentioning

confidence: 99%

Untargeted profiling of Glycyrrhiza glabra extract with comprehensive two‐dimensional liquid chromatography‐mass spectrometry using multi‐segmented shift gradients in the second dimension: Expanding the metabolic coverage

et al. 2018

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Metabolic profiling of Glycyrrhiza glabra using comprehensive two-dimensional liquid chromatography (LC × LC) coupled with photodiode array (PDA) and mass spectrometry (MS) detection is described. The separation was conducted under reversed-phase conditions, using a combination of first dimension ( D) 150 mm microbore cyano column utilising 2.7 μm diameter (d ) particles, and second dimension ( D) 50 mm superficially porous octadecylsilica column with 2.7 μm d particles. A multi-segmented shift gradient (MSG) for the D separation was developed, and the orthogonality achieved was compared with other modes of gradients, such as full in-fraction, and shift gradient systems. Results demonstrated a significant expansion of metabolic coverage using MSG in D, providing the highest measure of orthogonality compared to other gradient modes. Compound identifications were performed by employing complementary data from PDA and MS detection, with reference to structural group-type distribution in 2D space. A total of ca. 120 compounds were detected, and among them 37 were tentatively identified, distributed over the chemical families of glycosylated flavanones, triterpene saponins, and others. In comparison with one-dimensional LC, the total number of compounds detected was ca. 2-fold greater when LC × LC was employed. To the best of our knowledge, this is the first demonstration of the MSG mode in LC × LC, representing a powerful strategy to expand the metabolic coverage for analysis of plant-derived extracts, containing a multitude of different phytochemical classes.

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Separation and analysis of phenolic acids from Salvia miltiorrhiza and its related preparations by off-line two-dimensional hydrophilic interaction chromatography × reversed-phase liquid chromatography coupled with ion trap time-of-flight mass spectrometry

Cited by 54 publications

References 48 publications

Characterization of metabolites in rats after intravenous administration of salvianolic acid for injection by ultra‐performance liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Characterization of metabolites in rats after intravenous administration of salvianolic acid for injection by ultra‐performance liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Pharmacokinetics of salvianolic acid B, rosmarinic acid and Danshensu in rat after pulmonary administration of Salvia miltiorrhiza polyphenolic acid solution

Untargeted profiling of Glycyrrhiza glabra extract with comprehensive two‐dimensional liquid chromatography‐mass spectrometry using multi‐segmented shift gradients in the second dimension: Expanding the metabolic coverage

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