Separate Sensory Pathways for Photomorphogenesis in Phycomyces

Flores, Rafael; Cerdá‐Olmedo, Enrique; Corrochano, Luis M.

doi:10.1562/0031-8655(1998)067<0467:sspfpi>2.3.co;2

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“…mRNA isolation and cDNA synthesis Plates (internal diameter 8.5 cm) of minimal agar were inoculated with 10 5 heat-activated spores (48°C, 15 min) and incubated at 22°C in the dark (Corrochano and Cerda´-Olmedo 1988). After 48 h of growth, two plates placed in a black wooden box were simultaneously irradiated for 1 h (2.3·10 4 J/m 2 ) through an upper window carrying a filter holder (Flores et al 1998). Blue light from a quartz halogen lamp installed in a slide projector was passed through two heat filters and a broadband blue filter with maximum transmission at 440 nm (Corrochano and Cerda´-Olmedo 1990).…”

Section: Strainsmentioning

confidence: 99%

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Photomorphogenesis in Phycomyces: differential display of gene expression by PCR with arbitrary primers

Corrochano

2002

Mol Gen Genomics

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The zygomycete fungus Phycomyces blakesleeanus develops two types of fruiting bodies of very different size, macrophores and microphores. Blue light stimulates macrophorogenesis and inhibits microphorogenesis. I have adapted a method based on the polymerase chain reaction with arbitrary primers to investigate the role of differential gene expression during photophorogenesis in Phycomyces. Several cDNAs for genes induced in vegetative mycelium have been observed, but only one gene induced by blue light has been detected. I have demonstrated the feasibility of this approach by the isolation of a cDNA segment for the heat-shock protein HSP100 that is induced by blue light at the onset of sporangiophore development. The heat-shock protein HSP100 is an ATP-binding protein that has the ability to disassemble protein complexes. In plants, the gene for HSP100 is induced by light. The cDNA segment for HSP100 obtained from Phycomyces is 686 bp long and the predicted amino acid sequence contains one of the ATP-binding sites.

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Section: Strainsmentioning

confidence: 99%

“…The pleiotropy of mutations that affect more than one photoresponse has suggested that the Phycomyces phototransduction pathways rely on the combinatorial usage of several gene products (Flores et al 1998;Cerda´-Olmedo 2001;Cerda´-Olmedo and Corrochano 2001).…”

Section: Introductionmentioning

confidence: 99%

Photomorphogenesis in Phycomyces: differential display of gene expression by PCR with arbitrary primers

Corrochano

2002

Mol Gen Genomics

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Separate Sensory Pathways for Photomorphogenesis in Phycomyces

Cited by 1 publication

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Photomorphogenesis in Phycomyces: differential display of gene expression by PCR with arbitrary primers

Photomorphogenesis in Phycomyces: differential display of gene expression by PCR with arbitrary primers

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