Separate <i>cis</i> sequences and <i>trans</i> factors direct metabolic and developmental regulation of a potato tuber storage protein gene

Grierson, Claire; Du, Jian‐Sheng; Zabala, Marta de Torres; Beggs, Kyle T.; Smith, Caroline; Holdsworth, Michael J.; Bevan, Michael W.

doi:10.1046/j.1365-313x.1994.5060815.x

Cited by 173 publications

(125 citation statements)

References 21 publications

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“…Nuclear proteins were extracted from mature, dormant embryos imbibed for 6 h or young, expanding leaves as described (18). Proteins were precipitated from nuclear extracts in a 40% saturated ammonium sulfate solution to remove histones.…”

Section: Methodsmentioning

confidence: 99%

Transcripts of Vp - 1 homeologues are misspliced in modern wheat and ancestral species

McKibbin

Wilkinson

Bailey

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

146

154

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The maize (Zea mays) Viviparous 1 (Vp1) transcription factor has been shown previously to be a major regulator of seed development, simultaneously activating embryo maturation and repressing germination. Hexaploid bread wheat (Triticum aestivum) caryopses are characterized by relatively weak embryo dormancy and are susceptible to preharvest sprouting (PHS), a phenomenon that is phenotypically similar to the maize vp1 mutation. Analysis of Vp-1 transcript structure in wheat embryos during grain development showed that each homeologue produces cytoplasmic mRNAs of different sizes. The majority of transcripts are spliced incorrectly, contain insertions of intron sequences or deletions of coding region, and do not have the capacity to encode full-length proteins. Several VP-1-related lower molecular weight protein species were present in wheat embryo nuclei. Embryos of a closely related tetraploid species (Triticum turgidum) and ancestral diploids also contained misspliced Vp-1 transcripts that were structurally similar or identical to those found in modern hexaploid wheat, which suggests that compromised structure and expression of Vp-1 transcripts in modern wheat are inherited from ancestral species. Developing embryos from transgenic wheat grains expressing the Avena fatua Vp1 gene showed enhanced responsiveness to applied abscisic acid compared with the control. In addition, ripening ears of transgenic plants were less susceptible to PHS. Our results suggest that missplicing of wheat Vp-1 genes contributes to susceptibility to PHS in modern hexaploid wheat varieties and identifies a possible route to increase resistance to this environmentally triggered disorder.T he Viviparous1 (Vp1) gene is a major regulator of late embryo development in maize. Inactivation of this locus leads to disruption of embryo maturation, resulting in the promotion of germination of embryos while still attached to the cob (vivipary). The phenotypes of vp1 mutants show that this locus performs two distinct functions: to promote embryo maturation and embryo dormancy and simultaneously to repress germination (1, 2). Specific genes and enzyme activities have been identified that are activated or repressed by Vp1 in vivo. Expression of several enzymes (3) and maturation-related genes (4) is reduced in the mutants including those encoding seed-storage proteins and diverse LEA (late embryogenesis-abundant) proteins (1). On the other hand, ␣-amylase activity increases in the same mutants during aleurone maturation (3). These observations indicate a bifunctional role for Vp1 in regulating the balance between the activation of embryo-maturation genes and the repression of germinative hydrolases during kernel development and maturation.Orthologues of Vp1 have been cloned from rice [Osvp1 (4)], wild oat [AfVp1 (5)], and several dicotyledonous species such as Arabidopsis [ABI3 (6)] and poplar [PtABI3 (7)]. Vp1 homeologues have been mapped in wheat to the long arms of the group 3 chromosomes at a location conserved relative to Vp1 in maize and Osvp1 ...

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Section: Methodsmentioning

confidence: 99%

Transcripts of Vp - 1 homeologues are misspliced in modern wheat and ancestral species

McKibbin

Wilkinson

Bailey

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

146

154

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“…Whether these mRNA sequences are functional remain to be determined. Promoter analysis of the sugar up-regulated patatin class-I gene identified two separate cis-sequences responsible for sugar induction (Grierson et al, 1994). The cis-sequences contain two conserved 9-bp Suc response elements (SURE), which are similar to some motifs present in the promoters of some SU genes, e.g.…”

Section: Sugars Regulate Gene Expression Through Common Control Mechamentioning

confidence: 99%

Sugar Coordinately and Differentially Regulates Growth- and Stress-Related Gene Expression via a Complex Signal Transduction Network and Multiple Control Mechanisms

et al. 2001

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In plants, sugars are required to sustain growth and regulate gene expression. A large set of genes are either up-or down-regulated by sugars; however, whether there is a common mechanism and signal transduction pathway for differential and coordinated sugar regulation remain unclear. In the present study, the rice (Oryza sativa cv Tainan 5) cell culture was used as a model system to address this question. Sucrose and glucose both played dual functions in gene regulation as exemplified by the up-regulation of growth-related genes and down-regulation of stress-related genes. Sugar coordinately but differentially activated or repressed gene expression, and nuclear run-on transcription and mRNA half-life analyses revealed regulation of both the transcription rate and mRNA stability. Although coordinately regulated by sugars, these growth-and stress-related genes were up-regulated or down-regulated through hexokinase-dependent and/or hexokinaseindependent pathways. We also found that the sugar signal transduction pathway may overlap the glycolytic pathway for gene repression. ␣-Amylase and the stress-related genes identified in this study were coordinately expressed under sugar starvation, suggesting a convergence of the nutritional and environmental stress signal transduction pathways. Together, our studies provide a new insight into the complex signal transduction network and mechanisms of sugar regulation of growth and stress-related genes in plants.

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“…WRKY proteins can activate or repress transcription (Rushton et al, 2010). A WRKY transcription factor in barley, SUSIBA2, has been shown to bind to W-box and sucrose-responsive elements (SUREs) (Grierson et al, 1994) in the promoter of the isoamylase1 gene as an activator (Sun et al, 2003). A WRKY transcription factor in sweet potato, SPF1, is able to bind to the sugarresponsive sporamin and β-amylase promoters (Ishiguro and Nakamura, 1994), and this suggests that SPF1 acts as a repressor (Rook et al, 2006).…”

mentioning

confidence: 99%

Activation of ADP-Glucose Pyrophosphorylase Gene Promoters by a WRKY Transcription Factor, AtWRKY20,in Arabidopsis thalianaL. and Sweet Potato (Ipomoea batatasLam.)

Nagata

Hara

Saitou

et al. 2012

Plant Production Science

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Separate cis sequences and trans factors direct metabolic and developmental regulation of a potato tuber storage protein gene

Cited by 173 publications

References 21 publications

Transcripts of Vp - 1 homeologues are misspliced in modern wheat and ancestral species

Transcripts of Vp - 1 homeologues are misspliced in modern wheat and ancestral species

Sugar Coordinately and Differentially Regulates Growth- and Stress-Related Gene Expression via a Complex Signal Transduction Network and Multiple Control Mechanisms

Activation of ADP-Glucose Pyrophosphorylase Gene Promoters by a WRKY Transcription Factor, AtWRKY20,in Arabidopsis thalianaL. and Sweet Potato (Ipomoea batatasLam.)

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