Activation of ADP-Glucose Pyrophosphorylase Gene Promoters by a WRKY Transcription Factor, AtWRKY20,<i>in Arabidopsis thaliana</i>L. and Sweet Potato (<i>Ipomoea batatas</i>Lam.)

Nagata, Takafumi; Hara, Hiromichi; Saitou, Kenji; Kobashi, Akiko; Kojima, Kengo; Yuasa, Takashi; Ueno, Osamu

doi:10.1626/pps.15.10

Cited by 22 publications

(17 citation statements)

References 35 publications

(41 reference statements)

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“…The results revealed that the SURE-Like element is involved in sucrose-mediated activation of gene expression, which is consistent with the previous study in Arabidopsis and carrot taproots (Kwak et al 2006). The involvement of the W-box in sucrose inducibility that we found here is similar to the previous finding in Arabidopsis and sweet potato (Nagata et al 2012). …”

Section: Transient Expression Analysis Of the Candidate Cis Elementssupporting

confidence: 93%

“…A WRKY transcription factor from Arabidopsis, AtRKY20, was proven to enhance the expression of Arabidopsis AGPase large subunit gene, ApL3, by directly binding to the ApL3 promoter and activating the transcription. Transient expression experiments demonstrated that AtWRKY20 also activated the promoter of sweet potato AGPase small subunit gene, IbAGP1, by directly interacting with the region between positions -623 and -490 in the IbAGP1 promoter (Nagata et al 2012). Moreover, the W-box of the IbAGP1 promoter bound by AtWRKY20 activator might be an enhancer in sweet potato (Nagata et al 2012).…”

Section: Gus Orfmentioning

confidence: 99%

“…Transient expression experiments demonstrated that AtWRKY20 also activated the promoter of sweet potato AGPase small subunit gene, IbAGP1, by directly interacting with the region between positions -623 and -490 in the IbAGP1 promoter (Nagata et al 2012). Moreover, the W-box of the IbAGP1 promoter bound by AtWRKY20 activator might be an enhancer in sweet potato (Nagata et al 2012). There are four W-box elements found in the IbAGP1 promoter, which are located in -1985 (IbAGP1-I), -1434 (IbAGP1-II), -750 (IbAGP1-III, IbAGP1-IV), and -578 (IbAGP1-V).…”

Section: Gus Orfmentioning

confidence: 99%

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Promoter analysis of the sweet potato ADP-glucose pyrophosphorylase gene IbAGP1 in Nicotiana tabacum

Zheng

Liu

et al. 2015

Plant Cell Rep

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The IbAGP1 gene of sweet potato ( Ipomoea batatas ) encodes the sucrose-inducible small subunit of ADP-glucose pyrophosphorylase. Through expression analysis of 5'-truncations and synthetic forms of the IbAGP1 promoter in transgenic tobacco, we show that SURE-Like elements and W-box elements of the promoter contribute to the sucrose inducibility of this gene. Sweet potato (Ipomoea batatas) contains two genes (IbAGP1 and IbAGP2) encoding the catalytically active small subunits of ADP-glucose pyrophosphorylase, an enzyme with an important role in regulating starch synthesis in higher plants. Previous studies have shown that IbAGP1 is expressed in the storage roots, leaves, and stem tissues of sweet potato, and its transcript is strongly induced by applying sucrose exogenously to detached leaves. To investigate the tissue-specific expression of the IbAGP1 promoter, a series of 5'-truncated promoters extending from bases -1913, -1598, -1298, -1053, -716, and -286 to base +75 were used to drive the expression of the β-glucuronidase reporter gene (GUS) in tobacco plants (Nicotiana tabacum). Histochemical and fluorometric GUS assays showed that (1) GUS expression driven by the longest fragment (1989 bp) of the IbAGP1 promoter was detected in vegetative tissues (roots, stems, leaves), (2) fragments extending to -1053 or beyond retained strong GUS expression in roots, stems, and leaves, whereas further 5'-deletions resulted in considerable reduction in GUS activity, and (3) the series of 5'-truncated promoters responded differently to exogenously applied sucrose. The 1989-bp IbAGP1 promoter contains five sequences (two AATAAAA, one AATAAAAAA, and two AATAAATAAA) that are similar to sucrose-responsive elements (SURE). These SURE-Like sequences are found at nucleotide positions -1273, -1239, -681, -610, and -189. Moreover, putative W-box elements are found at positions -1985, -1434, -750, and -578. Synthetic promoters containing tandem repeats of the 4X SURE-Like or 4X W-box upstream from a minimal CaMV35S promoter-GUS fusion showed significant expression in transgenic tobacco in response to exogenous sucrose. These results show that SURE-Like elements and W-box elements of the IbAGP1 promoter contribute to the sucrose inducibility of this gene.

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Section: Transient Expression Analysis Of the Candidate Cis Elementssupporting

confidence: 93%

Section: Gus Orfmentioning

confidence: 99%

Section: Gus Orfmentioning

confidence: 99%

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Promoter analysis of the sweet potato ADP-glucose pyrophosphorylase gene IbAGP1 in Nicotiana tabacum

Zheng

Liu

et al. 2015

Plant Cell Rep

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“…polymers of Fru) synthesis in grasses (Nagaraj et al, 2001;Noël et al, 2001). Suc also acts as a signaling molecule that initiates/activates starch synthesis by inducing the gene expression of AGPase large subunits, starch synthase (GBSS1) and b-amylase (Nakamura et al, 1991;Harn et al, 2000;Wang et al, 2001;Nagata et al, 2012), and by posttranslationally activating the AGPase enzyme by redox modification (Tiessen et al, 2002). The down-regulation of photosynthetic CO 2 fixation by Suc is a widely observed phenomenon (Van Oosten and Besford, 1994), as is the induction of genes Figure 5.…”

Section: Suc Favors Tag Biosynthesismentioning

confidence: 99%

Sugar Potentiation of Fatty Acid and Triacylglycerol Accumulation

Zhai

Liu

et al. 2017

Plant Physiol.

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Photosynthetically derived sugar provides carbon skeletons for lipid biosynthesis. We used mutants of Arabidopsis (Arabidopsis thaliana) and the expression of oleogenic factors to investigate relationships among sugar availability, lipid synthesis, and the accumulation of triacylglycerol (TAG) in leaf tissue. The adg1 mutation disables the small subunit of ADP-glucose pyrophosphorylase, the first step in starch synthesis, and the suc2 mutation disables a sucrose/proton symporter that facilitates sucrose loading from leaves into phloem. The adg1suc2 double mutant increases glucose plus sucrose content in leaves 80-fold relative to the wild type, total fatty acid (FA) content 1.8-fold to 8.3% dry weight, and TAG more than 10-fold to 1.2% dry weight. The WRINKLED1 transcription factor also accumulates to higher levels in these leaves, and the rate of FA synthesis increases by 58%. Adding tt4, which disables chalcone synthase, had little effect, but adding the tgd1 mutation, which disables an importer of lipids into plastids to create adg1suc2tt4tgd1, increased total leaf FA to 13.5% dry weight and TAG to 3.8% dry weight, demonstrating a synergistic effect upon combining these mutations. Combining adg1suc2 with the sdp1 mutation, deficient in the predominant TAG lipase, had little effect on total FA content but increased the TAG accumulation by 66% to 2% dry weight. Expression of the WRINKLED1 transcription factor, along with DIACYLGLYCEROL ACYLTRANSFERASE1 and the OLEOSIN1 oil body-associated protein, in the adg1suc2 mutant doubled leaf FA content and increased TAG content to 2.3% dry weight, a level 4.6-fold higher than that resulting from expression of the same factors in the wild type.

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“…Other sugars and osmotic controls are either less effective or ineffective. 12 As it can be envisaged from this revision, most of our knowledge about the intracellular signaling cascade initiated by Suc is mainly related to metabolic processes. Despite the importance of developmental changes for the plant life cycle, intracellular Suc signaling in meristematic cells is largely unknown.…”

Section: Disclosure Of Potential Conflicts Of Interestmentioning

confidence: 99%

Sucrose signaling in plants: A world yet to be explored

Tognetti

Pontis²,

Martínez-Noël³

2013

Plant Signaling & Behavior

166

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The role of sucrose as a signaling molecule in plants was originally proposed several decades ago. However, recognition of sucrose as a true signal has been largely debated and only recently this role has been fully accepted. The best-studied cases of sucrose signaling involve metabolic processes, such as the induction of fructan or anthocyanin synthesis, but a large volume of scattered information suggests that sucrose signals may control a vast array of developmental processes along the whole life cycle of the plant. Also, wide gaps exist in our current understanding of the intracellular steps that mediate sucrose action. Sucrose concentration in plant tissues tends to be directly related to light intensity, and inversely related to temperature, and accordingly, exogenous sucrose supply often mimics the effect of high light and cold. However, many exceptions to this rule seem to occur due to interactions with other signaling pathways. In conclusion, the sucrose role as a signal molecule in plants is starting to be unveiled and much research is still needed to have a complete map of its significance in plant function.

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Activation of ADP-Glucose Pyrophosphorylase Gene Promoters by a WRKY Transcription Factor, AtWRKY20,in Arabidopsis thalianaL. and Sweet Potato (Ipomoea batatasLam.)

Cited by 22 publications

References 35 publications

Promoter analysis of the sweet potato ADP-glucose pyrophosphorylase gene IbAGP1 in Nicotiana tabacum

Promoter analysis of the sweet potato ADP-glucose pyrophosphorylase gene IbAGP1 in Nicotiana tabacum

Sugar Potentiation of Fatty Acid and Triacylglycerol Accumulation

Sucrose signaling in plants: A world yet to be explored

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