Abstract:To search for myometrial candidate genes regulated by progesterone, we isolated annexin 2 cDNA by subtractive hybridization and cloning. We also examined the effect of estradiol and/or progesterone, individually or combined, on expressions of annexin 2 and its ligand protein, p11 in pregnant sheep intrauterine tissues. Annexin 2 is a Ca 2C -dependent phospholipid-binding protein which interacts with p11 to form a bivalent heterotetramer. The heterotetramer was indicated in the production of prostaglandins thro… Show more
“…Previous studies showed that ANXA2 expression could be induced by estrogen in different types of cells, such as myometrium cells (Zhang and Wu, 2007) and macrophages (Hwang et al, 2006). Our data indicated that the estrogen could also act in an autocrine manner to stimulate local ANXA2 expression in theca cells.…”
“…Previous studies showed that ANXA2 expression could be induced by estrogen in different types of cells, such as myometrium cells (Zhang and Wu, 2007) and macrophages (Hwang et al, 2006). Our data indicated that the estrogen could also act in an autocrine manner to stimulate local ANXA2 expression in theca cells.…”
“…Of course, further studies are required to better understanding of this mechanism. 59,61,72,110,112,[114][115][116] In 2007, Qi zhang et al 117 examined the myometrial genes (is the inner layer of the uterine wall which basically contains smooth muscle cells and its main function is stimulation of uterine contraction), which regulated by progesterone or estradiol. In other words, the purpose of this study was to examine the hypothesis of progesterone facilitating role on uterine changes based on decreasing or increasing of genes which are expressed in uterus.…”
It is well known that embryo implantation is a critical process in which embryo should be able to reach and attach to endometrium. Until now, various types of factors are involved in the regulation of this process. S100 proteins are calciumbinding proteins, which have vital roles in embryo implantation and have been considered as possible candidate markers for endometrial receptivity. However, studies regarding mode of actions of these proteins are scarce and more mechanistic insights are needed to clarify exact roles of each one of the S100 protein family. Understanding of function of these proteins in different compartments, stages, and phases of endometrium, could pave the way for conducting studies regarding the therapeutic significance of these proteins in some disorders such as recurrent implantation failure. In this review, we outlined roles and possible underlying mechanisms of S100 protein family in embryo implantation.
“…As noted above, however, in nonpregnant animals, progesterone may increase Ca 2+ current density (74). A recent study suggested that another mechanism whereby progesterone controls myometrial contractility is via enhanced annexin 2 expression (91). Annexin 2, with its involvement in the cytoskeleton and association with lipid rafts (see below), can affect phasic contractions (92).…”
The purpose of this review is to evaluate what is known and not known about the effects of oestrous and its hormones on uterine contractility and excitability, and to assess how important, hormonal differences in nonpregnant animals are to Ca 2+ signalling mechanisms. Although the physical effects of oestrous on the uterus and the underlying hormonal changes have been known for many decades, there have been few attempts to integrate this knowledge with the molecular and cellular information more recently gathered on the mechanisms of excitation-contraction (EC) coupling. Many reports have advanced our understanding of differences in EC coupling between pregnant and nonpregnant uterus, but few have specified the hormonal status of the nonpregnant animal. Does this have consequences for the conclusions drawn? In this brief review, we firstly overview EC-coupling and then describe the oestrous cycle and its effects on mechanical activity. We then look in detail at the effects of hormonal changes on Ca 2+ entry and excitability in uterine cells, effects on internal Ca 2+ release from the sarcoplasmic reticulum (SR) and then review information about electrical activity at different stages of oestrous. We then examine recent evidence that effects of hormonal changes may be mediated through membrane lipid rafts and caveolae. We present our findings on myometrial Ca 2+ signalling at different stages of oestrous and use them as a focus to summarise the main findings of this review. We hope that this will be a stimulant to our colleagues studying myometrial physiology and pathophysiology; to deepen understanding and increase appreciation of the possible effects of oestrous and sex hormones on their studies.Excitation-contraction coupling in the uterus: an overview Several reviews on this topic have recently been published and should be consulted for details and additional references (1-8
Journal of NeuroendocrinologyCorrespondence to: Susan Wray, Department of Physiology, The University of Liverpool, Liverpool L69 3BX, UK (e-mail: s.wray@liv.ac.uk).In this review, we examine how far the increased understanding that we have of the events in excitation contraction can explain the effects of the oestrous cycle and sex hormones on uterine function. Observational studies of electrical and mechanical activity in the rat myometrium have shown a relative quiescence during pro-oestrous, with little propagation of any electrical events. Thus, uterine activity can be said to approximately inversely reflect plasma 17b-oestradiol concentrations. We show that Ca 2+ signalling and mechanical activity are greatest in metoestrous and dioestrous compared to pro-oestrous and oestrous. These data are discussed in terms of hormonal effects on Ca 2+ and K + channels. Finally, the influence of sex hormones on lipid rafts and caveolae are considered and discussed in relation to recent findings on their role in uterine signalling and contractility, and cholesterol levels and obesity.
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