Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s

Abstract

A cross‐reactive optical sensor array based on poly( p ‐phenyleneethynylene)s (PPEs) determines Edman degraded amino acids. We report a sensor array composed of three anionic PPEs P1–P3 , and their electrostatic complexes with metal ions (Fe 2+ , Cu 2+ , Co 2+ ). We recorded distinct fluorescence intensity response patterns as “fingerprints” of this chemical tongue toward standard phenylthiohydantoin (PTH) amino acids—degradation products of the Edman process. These “fingerprints” were converted into canonical scores by linear discrimination analysis (LDA), which differentiates all of the PTH‐amino acids. This array discriminates PTH‐amino acid residues degraded from an oligopeptide through Edman sequencing. This approach is complementary to chromatography approaches which rely on mass spectrometry; our array offers the advantage of simplicity.

Advances in the Use of Conducting Polymers for Healthcare Monitoring

“…Notably, CPs are emerging as pivotal materials for biosensors, and they are likely to attract investment in various fields. Some well-known CPs, including polyacetylene [36], polydi-acetylenes [37], polyaniline [38], polypyrrole [39], polythiophene [40], poly(3,4-ethylenedioxythiophene) [41], poly(phenylene vinylene) [42], poly(3-hexylthiophene-2,5-diy [43], polyindole [44], poly(p-phenylene) [45], poly(3-alkylthiophene) [46], poly(p-phenyleneterephthalamide) [47], poly(isothianaphthene), poly(α-naphthylamine), polyazulene [48], polyfuran, polyisoprene [49], polybutadiene [50], poly(3-octylthiophnene-3-methylthiophene) [51], polyorthotoluidiene [52], poly (dioctylfluorene) [53], poly(p-phenyleneethynylene) [54], polyphenylene sulfide [55], and poly(triaryl amine) [56], have found diverse applications in the physicochemical interface. Figure 2 illustrates 20 popular types of CPs used in sensors.…”

Machine Learning-Assisted Array-Based Detection of Proteins in Serum Using Functionalized MoS2 Nanosheets and Green Fluorescent Protein Conjugates

“…A signal transducer unit, which monitors the binding activity between the receptor and analytes, generates a pattern that can be recognized as specific to a certain analyte. In recent years, several receptors, such as fluorescent nanodots, 28 , 29 fluorescent conjugated polymers, 30 , 31 metal nanoparticles, 32 − 34 nanoclusters, 35 etc., have been reported as a platform for sensors for the classification of a wide range of analytes. Atomically thin two-dimensional (2D) materials provide wide active surfaces, which help them play a vital role in molecular recognition.…”

Cross-Reactive Fluorescent Sensor Array for Discrimination of Amyloid Beta Aggregates

“…PPE1 and PPESO3 are water-soluble conjugated polyelectrolytes (CPEs) with a polymeric p-phenylene ethynylene (PPE) backbone. CPEs are sensitive to minor conformational and environmental changes, making them suitable materials for sensing proteins, DNAs, and other disease-related biomarkers. , Recent studies showed that probes with similar structures, such as oligomeric p-phenylene ethynylene (OPE) and polythiophene (PT), , caused spectral shifts in Aβ aggregates, indicating the possibility of using PPEs as array elements. Using two probe pairs rather than four probes individually allows simultaneous observations of various parameters, including changes in fluorescence intensity and spectral shifts, and produces additional dimensionality in the sensor array (Figure b).…”