Sensitivity of Larvae and Adult and the Immunologic Characteristics of<i>Litopenaeus vannamei</i>under the Acute Hypoxia

Zhou, Hailong; Li, Yuhu; Wei, Lin; Zhang, Zhihuai; Huang, Hao; Xiang, Jianhai

doi:10.1155/2014/574534

Cited by 8 publications

(5 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have reported the transcriptome [19,20] and proteome [21][22][23] of V. harveyi-infected shrimp. However, the few NMR metabolomics studies conducted on shrimp thus far have focused on L. vannamei exposed to sulphide toxicity [24], hypoxia [25], Vibrio alginolyticus [26], and white spot syndrome virus (WSSV) infection [27].…”

Section: Introductionmentioning

confidence: 99%

Vibrio harveyi Infection Significantly Alters Amino Acid and Carbohydrate Metabolism in Whiteleg Shrimp, Litopenaeus vannamei

Kim

Lee

et al. 2020

Metabolites

View full text Add to dashboard Cite

Vibrio harveyi is one of the pathogens that threaten the shrimp farming industry. However, metabolic changes induced by V. harveyi infection in shrimp remain unknown. In this study, we first conducted high resolution-magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR)-based metabolomics studies on gill, hepatopancreas, and haemolymph of V. harveyi-infected white leg shrimp, Litopenaeus vannamei. Using multivariate statistical analysis, we observed a clear separation between the early (3 and 9 h post-injection (hpi)) and late phases (24, 72 and 144 hpi) of the infection in all tissues. Moreover, metabolic changes in response to V. harveyi infection were faster in the haemolymph in the early phase and significantly changed in the late phase of the infection in the gills. Extensive changes were observed in the hepatopancreas, with 24 hpi being the turning point of progression from early to late phase infection in the hepatopancreas. V. harveyi infection increased the energy demand in L. vannamei and the amino acid and carbohydrate metabolism pathways also exhibited significant changes depending on the tissue. Thus, each tissue displayed different metabolic changes, depending on the progress of the infection.

show abstract

Section: Introductionmentioning

confidence: 99%

Vibrio harveyi Infection Significantly Alters Amino Acid and Carbohydrate Metabolism in Whiteleg Shrimp, Litopenaeus vannamei

Kim

Lee

et al. 2020

Metabolites

View full text Add to dashboard Cite

show abstract

“…HC is a major component of haemolymph, which is an efficient biological defence weapon utilized in invertebrate innate immunity (Coates & Nairn, ; Destoumieux‐Garzón et al, ; Qiao et al, ; Zhang et al, ). HC can exert respiratory functions in shrimp (Cheng, Liu, & Kuo, ; Coates & Nairn, ; Zhou et al, ), and can be synthesized in crustacean hepatopancreas (Lee, Lee, & Söderhäll, ; Ward et al, ). It was found that in the case of hypoxia, shrimp with lower HC concentration has a greater need to synthesize more HC to maintain oxygen delivery (McMahon, ; Tommerdahl, Burnett, & Burnett, ).…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary supplementation of Astragalus membranaceus , Codonopsis pilosula , and Glycyrrhiza uralensis extract mixture on growth performance, haematological parameters and hepatopancreatic performance in juvenile Pacific white shrimp ( Litopenaeus vannamei )

et al. 2019

View full text Add to dashboard Cite

Pacific white shrimp is the major farmed shrimp species in the world. It is known to be very sensitive to the environmental and management changes such as intensification, which is one of the primary necessities to increase shrimp production, but represents a stressful condition, and needs to be managed properly to diminish its negative effects in aquaculture. In this study, juvenile Pacific white shrimp were fed diets supplementing with equal quantities of Astragalus membranaceus, Codonopsis pilosula and Glycyrrhiza uralensis extracts in different concentrations (0, 1.0, 2.0 and 3.0 g/kg) for 8 weeks. We subsequently estimated the effects of supplemented diets on growth performance, haematological parameters, and histological changes of hepatopancreas. Results revealed that the supplemented mixture had no benefits on growth and survival rate, but had favourable effects on haemocyte count, number of granulocytes, and hemocyanin concentration in the haemolymph (p > 0.05). We observed that R cells and B cells were increased in hepatopancreatic tubules of shrimps fed on supplemented diets. Besides, a diet containing 2.0 g/kg of extract mixture maintained the decreased levels of alanine aminotransferase (p < 0.05) and aspartate aminotransferase (p > 0.05). Among the used concentrations, 2.0 g/kg seemed to be the most suitable concentration. K E Y W O R D S Astragalus membranaceus, Codonopsis pilosula, Glycyrrhiza uralensis, histology, immune response, Litopenaeus vannamei How to cite this article: Prathomya P, Hussein HA, Tran NT, et al. Effects of dietary supplementation of Astragalus membranaceus, Codonopsis pilosula, and Glycyrrhiza uralensis extract mixture on growth performance, haematological parameters and hepatopancreatic performance in juvenile Pacific white shrimp (Litopenaeus vannamei). Aquac Res.

show abstract

“…). Hypoxic exposure has been shown to increase the expression and concentration of haemocyanin (Brouwer et al ., ; Zhou et al ., ), as well as haemocyanin oxygen affinity in crabs and shrimps (Lallier & Truchot, ). This response of haemocyanin to hypoxia could be activated by HIF‐1, which can bind with HIF‐1 responsive elements probably present in the promoters of Hc1 and Hc2 genes (Semenza et al , ).…”

Section: Discussionmentioning

confidence: 99%

Haemocyanin is essential for embryonic development and survival in the migratory locust

Chen

et al. 2015

Insect Molecular Biology

View full text Add to dashboard Cite

Haemocyanins are commonly known as copper-containing oxygen carriers within the haemolymph of arthropods, and have been found in many orders of insects. However, it remains unresolved why haemocyanins persist in insects that possess elaborate tracheal systems for oxygen diffusion to cells. Here we identified haemocyanins in the migratory locust Locusta migratoria that consists of two distinct subunits, Hc1 and Hc2. Genomic sequence analysis indicated that Hc1 and Hc2 have four and three gene copies, respectively, which may have evolved via gene duplication followed by divergent evolution of introns. The two subunits exhibit abundant and embryonic-specific expression at the mRNA and protein level; their expression peaks in the mid-term embryo and is not detectable in the late nymphal and adult stages. A larger proportion of the haemocyanins is present in the yolk compared with that in the embryo. Immunostaining shows that haemocyanins in the embryo are mainly expressed in the epidermis. Knockdown of Hc1 and Hc2 results in significant embryonic developmental delay and abnormality as well as reduced egg hatchability, ie the proportion of hatched eggs. These results reveal a previously unappreciated and fundamental role for haemocyanins in embryonic development and survival in insects, probably involving the exchange of molecules (eg O2 ) between the embryo and its environment.

show abstract

Sensitivity of Larvae and Adult and the Immunologic Characteristics ofLitopenaeus vannameiunder the Acute Hypoxia

Cited by 8 publications

References 47 publications

Vibrio harveyi Infection Significantly Alters Amino Acid and Carbohydrate Metabolism in Whiteleg Shrimp, Litopenaeus vannamei

Vibrio harveyi Infection Significantly Alters Amino Acid and Carbohydrate Metabolism in Whiteleg Shrimp, Litopenaeus vannamei

Effects of dietary supplementation of Astragalus membranaceus , Codonopsis pilosula , and Glycyrrhiza uralensis extract mixture on growth performance, haematological parameters and hepatopancreatic performance in juvenile Pacific white shrimp ( Litopenaeus vannamei )

Haemocyanin is essential for embryonic development and survival in the migratory locust

Contact Info

Product

Resources

About