Sensitivity of immunoperoxidase and immunofluorescence staining for detecting chlamydia in conjunctival scrapings and in cell culture.

Woodland, Ralph M.; El-Sheikh, H.; Darougar, S; Squires, S.

doi:10.1136/jcp.31.11.1073

Cited by 18 publications

(3 citation statements)

References 7 publications

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“…Direct detection of cytoplasmic inclusions was achieved by a variety of stains (Giemsa, Castañ eda, Machiavello, Stamp, Giménez). The introduction of the immunofluorescence (Woodland et al, 1978) using polyclonal or monoclonal antibodies against the major outer membrane protein (MOMP), which is conjugated with fluorescein isothiocyanide, has been an additional step to further improve specificity and sensitivity of cell culture assays (Langhammer, 1989;Eidebenz, 1991;Fischer, 1992;Dove, 1995;Unkrig, 1995;Andersen et al, 1997;Andersen, 2000).…”

Section: Aetiologic Aspects Of Chlamydiamentioning

confidence: 99%

Avian host range of Chlamydophila spp. based on isolation, antigen detection and serology

2003

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Published reports and our own diagnostic data on the avian host range of avian Chlamydophila spp. are presented in an attempt to provide evidence for the large number of bird species that have been naturally infected with chlamydia. The term 'chlamydia-positive' is based on either isolation of the organism and antigen detection or on serological detection of circulating antibodies. The list of chlamydia-positive birds contains the six major domestic species (chicken, turkey, Pekin duck, Muscovy duck, goose, and pigeon), the three minor domestic species (Japanese quail, bobwhite quail, and peafowl) and a total of 460 free-living or pet bird species in 30 orders. The order Psittaciformes contains by far the most (153 of 342; 45%) chlamydia-positive bird species. More than 20% of all species per order are positive for chlamydia in the orders Lariformes (gulls, 26 of 92 species; 28%), Alciformes (alks, six of 23 species; 26%), Sphenisciformes (penguins, four of 16 species; 25%), and Anseriformes (ducks and geese, 33 of 157 species; 21%). Only 5% of all bird species (14 of 259 species) in the order Phasianiformes (gallinaceus birds) are chlamydia-positive. The different percentages of chlamydia-positive bird species reflect: (i) a high rate of investigations (e.g. of domestic birds) compared with infrequent testing (e.g. of Charadriiformes or Cuculiformes), (ii) frequent zoonotic implications (e.g. psittacine and columbiform birds), and (iii) an assumed high susceptibility to infection and subsequent seroconversion (e.g. waterfowl).

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Section: Aetiologic Aspects Of Chlamydiamentioning

confidence: 99%

Avian host range of Chlamydophila spp. based on isolation, antigen detection and serology

2003

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“…They reported that IF staining had been shown to be sufficiently more sensitive and rapid than Giemsa or iodine staining in the detection of C. trachomatis inclusions. On the other hand, earlier reports have also shown that IF staining is more sensitive and specific for the direct detection of C. psittaci inclusions in the clinical specimens (Woodland et al 1978 ;Hanna et al 1965). However, it has been difficult to detect inclusions in conjunctival scrapings from newborns.…”

Section: Discussionmentioning

confidence: 89%

An application of fluorescein-conjugated monoclonal antibodies to the diagnosis of Chlamydia trachomatis infection in children.

Numazaki

Chiba

Moroboshi

et al. 1985

Tohoku J. Exp. Med.

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“…Giemsa staining has been found to be slightly less sensitive than the immunocytochemical methods but is more convenient for clinic use. Moreover, problems of artifact are minimal with the Giemsa method (Woodland et al, 1978). Inoculation of monolayer cultures of irradiated McCoy cells with conjunctival swabbings provides another method of detecting C. trachomatis, but facilities for this investigation are available in only a few specialist laboratories, thereby emphasing the value of cytology in routine clinical practice.…”

Section: Diagnosis Of Oculoviral Infectionmentioning

confidence: 99%

Cytological diagnosis of virus-infected cells in Papanicolaou smears and its application in clinical practice.

Coleman¹

1979

J Clin Pathol

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SUMMARY The cytopathic changes of herpes simplex virus, varicella-zoster virus, the human polyomaviruses, and the trachoma agent can be recognised in smears prepared from a wide variety of specimens. The morphology of virus-infected cells is described, and the clinical value of these observations is discussed. Cytological diagnosis offers a rapid and convenient method of detecting infection with these viruses which can be confirmed by conventional virological method while the patient is still in the acute stages of the disease. Confirmation can also be made retrospectively by reprocessing cells, identified in the Papanicolaou smear, for electron microscopy. Screening for virus-infected cells in cytological smears is a technique that can be developed in any pathology laboratory prepared to provide a comprehensive cytology service, and it is proving to be a useful tool in clinical diagnosis and applied research.In recent years, cytopathologists have observed that the alterations in cell morphology that accompany certain virus infections can be recognised in Papanicolaou smears submitted to routine examination by light microscopy. The cytopathic changes due to herpes simplex virus, varicella-zoster virus, cytomegalovirus, and the human polyomaviruses have been described in epithelial cells in cytological specimens such as cervical smears, urine, sputum, and conjunctival scrapes, and the presence of virusinfected cells in the smears has been shown to be a convenient and reliable guide to active infection of the patient with these viruses. These observations have widened the scope of the cytodiagnostic technique for they imply that the large numbers of cytological specimens that are submitted routinely for examination for malignant cells can at the same time be screened for evidence of viral activity.The aim of this paper is to describe the morphology of virus-infected cells in Papanicolaou-stained smears and to discuss the value of these observations in clinical practice. The identification of cytological markers of virus disease can alert the clinician to viral illness in his patient when none is suspected and can provide him with a differential diagnosis when

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Sensitivity of immunoperoxidase and immunofluorescence staining for detecting chlamydia in conjunctival scrapings and in cell culture.

Abstract: (Kurstak et al., 1975), and to study the development cycle of Chlamydia psittaci in peritoneal macrophages of infected mice (Mields et al., 1974

Cited by 18 publications

References 7 publications

Avian host range of Chlamydophila spp. based on isolation, antigen detection and serology

Avian host range of Chlamydophila spp. based on isolation, antigen detection and serology

An application of fluorescein-conjugated monoclonal antibodies to the diagnosis of Chlamydia trachomatis infection in children.

Cytological diagnosis of virus-infected cells in Papanicolaou smears and its application in clinical practice.

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