Sensitivity of human colon tumor metastases to anticancer drugs in athymic (nude) mice

High expression of Fatty Acid‐Binding Protein 5 promotes cell growth and metastatic potential of colorectal cancer cells

“…As shown in Fig. 1B,C, FABP5 is markedly upregulated in CRC cells, especially in the highly metastatic HCT116 cells, compared to the nonmetastatic Caco‐2 cells 27, 28 and normal colon fibroblasts (CCD‐18Co). These results clearly suggest that the FABP5 gene is upregulated in CRC cells and that its level of expression may correlate positively with disease stage.…”

Activation of c-Src by receptor tyrosine kinases in human colon cancer cells with high metastatic potential

“…The SW 480 and SW 620 human colon cancer cell lines have also been well-studied (Co ey et al, 1987) and their behavior in the nude mouse intrasplenic injection assay is predictable (Shah et al, 1994;Witty et al, 1994;Zirvi et al, 1993). The SW 480 cell line was derived from a primary Dukes' C colon cancer and the SW 620 cell line was derived from a lymph node metastasis in the same patient.…”

Systemic TNFα Gene Therapy Synergizes With Liposomal Doxorubicine in the Treatment of Metastatic Cancer

“…23 EGFR overexpressing LS174T human Colorectal cancer cells develop highly aggressive liver metastases several days after intrasplenic injection, and only 25% of metastases respond to doxorubicine treatment at a dose of 10 mg/kg. 32 Access for macromolecular drugs to LS174T metastases is also limited: with a CEA antibody, diffusion into larger tumor nodules was hampered, and the maximal diffusion path length achieved after 24 hours was on average 400 µm. 33 Hence, diffusion of 100 nm liposomes like Doxil is expected to be inefficient.…”

“…For the liver metastasis model of human LS174T-lenti-luc human colon carcinoma, 7.5 × 10 5 cells resuspended in 50 µl PBS were injected into the spleen of 6-week-old female NMRI nu/nu mice analogously as described. 32 Growth of metastases was analyzed by BLI (see below).…”