Sensitivity of hiPSC-derived neural stem cells (NSC) to Pyrroloquinoline quinone depends on their developmental stage

Functional properties of different collagen scaffolds to create a biomimetic niche for neurally committed human induced pluripotent stem cells (iPSC)

“…The process of neural stem cell commitment from iPSCs was rapid (7 days of neural induction) and effective (we were able to obtain approximately 20 million of hNSC from one million of hiPSCs). In contrast to hiPSC, the obtained human iPSC-derived neural stem cells (hiPSC-NSC) did not express pluripotency markers OCT4 or NANOG [2], while the multipotent neural differentiation was manifested by expression of markers typical for a neural stem cell population (NESTIN), as well as markers for neuronal (β-TUBULIN III, DOUBLECORTIN, MAP-2) and glial differentiation (GFAP, GalC) (Fig. 1A).…”

“…3 and 5), which binds to the nuclear protein MKI67, present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting cells (G0) [34]. Markers applied to detect neural stem/progenitor cells (NESTIN) and their commitment into neuronal (β-TUBULIN III, NF200, DOUBLE-CORTIN, MAP-2), astrocytic (GFAP) and oligodendroglial (PDGFRα, GalC) lineages have been used before in the study of our group [2,36,46] and are typical for characterization of neural lineages in vitro and in vivo [31]. It is important to note that the results of this study based on immunofluorescent images are only qualitative due to the high autofluorescence of the Col-DAC scaffolds (Figs.…”

Bezafibrate Upregulates Mitochondrial Biogenesis and Influence Neural Differentiation of Human-Induced Pluripotent Stem Cells

“…hiPSC (Gibco® Human Episomal iPSC Line, Life Technologies, Thermo Fisher Scientific) were differentiated into NSC, eNP, and late NP in accordance with the protocol of [13]) with some modifications as described previously [11, 12]. hiPSC at the undifferentiated stage of development were cultured in Essential 8 Medium (Thermo Fisher Scientific) on a 6-well plate covered with rh-Vitronectin (Thermo Fisher Scientific) with medium replaced every day.…”

“…After 5 days of exposition to BZ, samples were taken to evaluate its effects on the cells at the specific stage of development. The phenotype of the control populations used in the experiments of this study was characterized before exposition to BZ by RT-PCR, RNA-seq, and immunocytochemistry staining methods as previously described [11, 12]…”

“…We have shown that three cell populations obtained during early neural differentiation of hiPSC reveal distinct characteristic and differ significantly on the level of transcription of genes encoding pluripotency and neural differentiation markers. The cell phenotype was confirmed by immunofluorescence staining, RT-PCR, and RNA-seq [11, 12].

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