Sensitivity improvement of kukoamine determination by complexation with dihydrogen phosphate anions in capillary zone electrophoresis

Li, Yuanyuan; Rui, Di; Hsu, Wing-Leung; Huang, Yi‐Hui Christine; Sun, Hongyan; Cheung, Hon-Yeung

doi:10.1002/elps.201500030

Cited by 5 publications

(1 citation statement)

References 31 publications

(36 reference statements)

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“…Results obtained from 10 batches of LyC samples suggested that kukoamine metabolites were widespread in LyC, even though the kind and abundance of each compound varied remarkably in samples from batch to batch. KA and KB were predominant in all samples, and KB was more abundant than KA. , Compound E8 , N 1 , N 10 -bis(dihydrocaffeoyl)spermidine, was also found in all batches of samples with a content close to kukoamines A and B. However, other kinds of kukoamine metabolites are very rare in LyC.…”

Section: Results and Discussionmentioning

confidence: 82%

Identification and Characterization of Kukoamine Metabolites by Multiple Ion Monitoring Triggered Enhanced Product Ion Scan Method with a Triple-Quadruple Linear Ion Trap Mass Spectrometer

Wang

Zhao

et al. 2015

J. Agric. Food Chem.

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Kukoamines are a series of bioactive phytochemicals conjugated by a polyamine backbone and phenolic moieties. Understanding the structural diversity of kukoamine metabolites in plants is meaningful for drug discovery. In this study, an LC-MS/MS method was established for kukoamine profiling and characterization from lycii cortex (LyC) via a triple-quadrupole linear ion trap mass spectrometry (Q-TRAP). On the basis of the typical fragmentation of kukoamine, a diagnostic ion, which represents the features of the backbone and phenolic substitute, was chosen as the product ion for precursor ion scan, and then the screened precursor ions were applied to a successive multiple ion monitoring triggered enhanced product ion scan (MIM-EPI) to simultaneously present the profile survey and MS/MS acquisition. Because the MIM narrowed the ion scan range in Q1 and the ion trap enhanced the ion fragments passing through Q2, the qualitative capability of quadrupole MS can be greatly improved, especially for capture of the uncommon metabolites. There are 12 kukoamine metabolites identified from LyC, with either spermine or spermidine backbone and with conjugation of one to three dihydrocaffeoyls or other kinds of phenolic moieties. Except for kukoamines A and B, other metabolites were identified in LyC for the first time. This approach can be utilized for metabolite identification in other substrates.

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Section: Results and Discussionmentioning

confidence: 82%