Sensitivity and Specificity of Urinary Cannabinoid Detection With Two Immunoassays After Controlled Oral Administration of Cannabinoids to Humans

Abstract

For forensic and clinical toxicologic purposes, cannabis consumption is screened using easy-to-handle immunoassays. The sensitivity and specificity of these immunoassays have not yet been established in samples from volunteers receiving oral synthetic tetrahydrocannabinol or cannabis extracts using liquid chromatography-mass spectrometry/mass spectrometry as the reference method. Urine samples were collected in an open, randomized, single-center, three-period crossover study including 18 healthy male volunteers given either 20 mg synthetic tetrahydrocannabinol (Marinol) as a control substance or five different types of Cannabis sativa extracts.

Direct ELISA kits as a sensitive and selective screening method for abstinence control in urine

“…ROC curves were used to confirm the cutoff values and to evaluate the discrimination power by ROC AUC. Several studies also used this tool to define the optimal cutoff value for their immunological tests [5,[10][11][12][13][14]. Furthermore we examined the Youden-Index as an additional marker for the performance of the test systems.…”

Abstinence phenomena of chronic cannabis-addicts prospectively monitored during controlled inpatient detoxification: Cannabis withdrawal syndrome and its correlation with delta-9-tetrahydrocannabinol and -metabolites in serum

“…Reference levels in serum were ≥1.0 ng/ml (THC and THC-OH) and ≥2.0 ng/ml (THC-COOH). For semiquantitative analysis, urine was investigated by Microgenics CEDIA DAU Multi-Level THC-assay (Microgenics) using a valid measurement range between 150 and 50 ng THC-COOH/ml urine (Grauwiler et al, 2008).…”

Cannabisbezogene Störungen

“…Als klinische Nachweismethode hat sich der semiquantitative Immuno-Assay bewährt (üblicher valider quantitativer Messbereich für THC-COOH im Urin: 150 -50 ng/ml). In der Praxis gilt ein Patient unter 50 ng/ml THC-COOH im Urin zumindest laborchemisch als "entgiftet"unterhalb dieses "cut-offs" steigt die Wahrscheinlichkeit falsch positiver Werte [7]. Ein beispielhafter Entgiftungsverlauf aus unserer Klinik ist in • ▶ Abb.…”