Sensitivity and specificity of BP180 NC16A enzyme-linked immunosorbent assay for the diagnosis of pemphigoid gestationis

Saif, F. Al; Jouen, F.; Hébert, V.; Chiavelli, Hélène; Darwish, Basma; Duvert‐Lehembre, S.; Joly, P.; Passeron, Thierry; Miséry, Laurent; Grob, Jean‐Jacques; Michel, Catherine; Estève, É.; Geillet, Gerard; Berbis, P.; Friedel, Jean; Maillard, H.; Stalder, J.‐F.; Dupin, Nicolas; Vabres, P.; Dereure, O.; Humbert, Philippe; Lagrange, Brigitte; Thomas, Luc; Saïag, Philippe; Machet, L.; Skowron, F.; Bernard, Philippe; Litrowski, N.; Verneuil, L.; Pallure, V.; Modiano, P.; Taı̈eb, Alain; Carpentier, O.; Schomoor, Pascal; D’Incan, M.

doi:10.1016/j.jaad.2016.09.030

Cited by 27 publications

(25 citation statements)

References 3 publications

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“…For pemphigoid diseases, ELISAs applying recombinant BP180 NC16A, BP230, and type VII collagen are commercially available (MBL, Euroimmun) . The BP180 NC16A ELISA yielded diagnostic sensitivities between 84 % and 89 % with BP and from 96 % to 97 % in pemphigoid gestationis . For BP, additional use of the BP230 ELISA increases the diagnostic sensitivity by about 5–8 % .…”

Section: Enzyme‐linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Diagnosis of autoimmune bullous diseases

Beek

Zillikens

Schmidt

2018

J Deutsche Derma Gesell

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Autoimmune bullous disorders (AIBDs) are a heterogeneous group of rare diseases clinically characterized by erosions and/or blisters on the skin and mucous membranes. AIBDs can be categorized into two groups: pemphigus diseases, characterized by intraepidermal blistering and autoantibodies against desmosomal proteins such as desmoglein (Dsg) 1, Dsg3, members of the plakin family, and subepidermal AIBDs, comprised of pemphigoid diseases and dermatitis herpetiformis. Autoantibodies in dermatitis herpetiformis target transglutaminases 2 and 3, while in pemphigoid disease, autoantibodies are directed against structural proteins of the dermal-epidermal junction. Although analysis of a perilesional biopsy with direct immunofluorescence (IF) microscopy is still the diagnostic gold standard, several assays have become widely available that allow serological diagnosis in the majority of patients. Standard serological diagnosis includes indirect IF on monkey esophagus and salt-split human skin. Assays to further characterize autoantibody specificity include ELISA systems based on recombinant forms of the immunodominant regions of the target antigens as well as multivariant indirect IF microscopy tests with several miniature substrates. These serological assays are complemented by various in-house assays using immunoblotting and ELISA, which are only available in specialized laboratories. Here we review new developments in the diagnosis of AIBDs and describe state-of-the-art diagnostic procedures for this group of diseases.

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Section: Enzyme‐linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Diagnosis of autoimmune bullous diseases

Beek

Zillikens

Schmidt

2018

J Deutsche Derma Gesell

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“…This test has shown a specificity of 94%–98% and a sensitivity of 86%–97% in the detection of BP180 antibodies in patients with PG. 33 – 35 ELISA is suitable to monitor disease activity because serum levels of anti-BP180 NC16A correlate with disease severity. 33 , 34 In a more recent study, routine immunohistochemistry showed a linear C4d immunoreactant deposition along the basement membrane in 100% of the patients with PG compared to 0% in patients with PEP.…”

Section: Diagnosismentioning

confidence: 99%

Pemphigoid gestationis: current perspectives

Sävervall

Sand

Thomsen

2017

CCID

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Many skin diseases can occur in pregnant women. However, a few pruritic dermatological conditions are unique to pregnancy, including pemphigoid gestationis (PG). As PG is associated with severe morbidity for pregnant women and carries fetal risks, it is important for the clinician to quickly recognize this disease and refer it for dermatological evaluation and treatment. Herein, we review the pathogenesis, clinical characteristics, and management of PG.

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“…Die kommerziell erhältlichen ELISAs für Pemphigoid‐Erkrankungen verwenden rekombinantes BP180 NC16A, BP230 und Kollagen Typ VII . Die diagnostische Sensitivität des ELISAs für BP180 NC16A lag beim BP zwischen 84 % und 89 % und beim Pemphigoid gestationis zwischen 96 % und 97 % . Der zusätzliche Einsatz des BP230‐ELISAs erhöht die diagnostische Sensitivität beim BP um etwa 5–8 % .…”

Section: Elisa (Enzyme‐linked Immunosorbent Assay)unclassified

Diagnostik blasenbildender Autoimmundermatosen

Beek

Zillikens

Schmidt

2018

J Deutsche Derma Gesell

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Blasenbildende Autoimmundermatosen (BAIDs) sind eine heterogene Gruppe seltener Erkrankungen, die klinisch durch Erosionen und/oder Blasen an Haut und Schleimhäuten charakterisiert sind. BAIDs können in zwei Gruppen eingeteilt werden: Pemphigus-Erkrankungen, die durch intraepidermale Blasenbildung und Autoantikörper gegen desmosomale Proteine wie Desmoglein (Dsg) 1, Dsg3 und Mitglieder der Plakin-Familie charakterisiert sind, sowie subepidermale BAIDs, die Pemphigoid-Erkrankungen und die Dermatitis herpetiformis umfassen. Bei der Dermatitis herpetiformis greifen die Autoantikörper die Transglutaminasen 2 und 3 an, während sie bei Pemphigoid-Erkrankungen gegen Strukturproteine der dermoepidermalen Junktionszone gerichtet sind. Die Analyse einer periläsionalen Biopsie mittels direkter Immunfluoreszenzmikroskopie (IF-Mikroskopie) stellt zwar nach wie vor den diagnostischen Goldstandard dar, mittlerweile sind jedoch verschiedene Tests allgemein verfügbar, die eine serologische Diagnosefindung für den Großteil der Patienten ermöglichen. Zur serologischen Standarddiagnostik gehört die indirekte IF auf Affenösophagus und NaCl-separierter Spalthaut. Die Spezifität der Autoantikörper kann mit ELISA-Systemen, die auf rekombinanten Formen der immundominanten Regionen der Zielantigene basieren, sowie indirekter IF-Mikroskopie anhand multivarianter Tests mit mehreren multivariate näher charakterisiert werden. Diese serologischen Tests werden durch verschiedene hauseigene Immunoblotting- und ELISA-Systeme ergänzt, die nur in spezialisierten Laboratorien verfügbar sind. In diesem Review geben wir einen Überblick über neue Entwicklungen bei der Diagnose von BAIDs und beschreiben moderne Diagnoseverfahren für diese Krankheitsgruppe.

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Sensitivity and specificity of BP180 NC16A enzyme-linked immunosorbent assay for the diagnosis of pemphigoid gestationis

Cited by 27 publications

References 3 publications

Diagnosis of autoimmune bullous diseases

Diagnosis of autoimmune bullous diseases

Pemphigoid gestationis: current perspectives

Diagnostik blasenbildender Autoimmundermatosen

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