Sensitivity and Limit of detection of biosensors based on ring resonators

Gandolfi, Davide; Guider, R.; Chalyan, Tatevik; Pavesi, L.; Pasquardini, Laura; Pederzolli, Cecilia; Samusenko, A.; Pucker, G.

doi:10.1109/aisem.2015.7066797

Cited by 7 publications

(9 citation statements)

References 3 publications

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“…The regeneration performances of Glycine concerning this aptamer were already demonstrated in [11]. An example of such measurement for Aflatoxin concentration of 12.5 nM (DMSO concentration 0.1% v/v) is presented in Figure 3.…”

Section: Aflatoxin Sensing Measurementsmentioning

confidence: 94%

Sensitivity and Limit of Detection of biosensors based on ring resonators

Gandolfi

Guider

Chalyan

et al. 2015

Sensing and Bio-Sensing Research

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In this work, we present a study of the Sensitivity (S) and Limit of Detection (LOD) of microring based photonic biosensors as a function of the waveguide composition and dimensions. The target is aflatoxin, which is a toxin of major concern for south Europe diary industry. The sensing device is based on an array of multiple SiON microring resonators, fiber-coupled to both an 850 nm VCSEL and a silicon photodetectors, packaged with a microfluidic circuit. Volumetric sensing with glucose-water solutions of various concentrations yields a best sensitivity of 112 nm/RIU. To link these results to the limit of detection of the sensors, we also measured the noise of our experimental readout system and then calculated the LOD of our sensors. We found a best value of LOD of 1.6x10 -6 RIU (referred to volumetric sensing). Finally, we detected aflatoxin in solutions of various concentrations (down to 1.58 nM) by functionalized sensors. The functionalization is based on a wet silanization and specific DNA-aptamer binding on the chip. Reproducibility and reusability of the sensor are investigated by several chemical treatments. Optimum procedure allows multiple sequential measurements with a good endurance.

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Section: Aflatoxin Sensing Measurementsmentioning

confidence: 94%

Sensitivity and Limit of Detection of biosensors based on ring resonators

Gandolfi

Guider

Chalyan

et al. 2015

Sensing and Bio-Sensing Research

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“…To determine the sensitivity of the ring resonator sensors, we monitored the shift of the WGM resonances while exposing the sensor to several glucose - water solutions of different concentrations. More details on this experiment are available in [ 13 ]. Figure 8 represents the bulk sensitivity of the L2 and L5 wafers as a function of the polarization.…”

Section: Sensitivity Sensing Measurements and Discussionmentioning

confidence: 99%

“…The limit of detection (LOD) of our sensors is defined as the minimum input quantity that can be distinguished with more than 99% fidelity [ 13 ], and it is determined as the ratio between three times the output uncertainty and the sensitivity of our sensor. In our case, we measured a LOD of 1.6 × 10 −6 RIU on sensors from wafer L5, and 3 × 10 −6 RIU with L2 samples.…”

Section: Sensitivity Sensing Measurements and Discussionmentioning

confidence: 99%

“…The final goal of our sensors is the selective sensing of Aflatoxin M1 in buffered solutions. To test the feasibility and the limits of such detection, we functionalized the samples by using a wet silanization process [ 13 ] using a DNA aptamer that recognizes AFM1 with high affinity and specificity [ 14 , 15 ] . The wet silanization process is followed by a deposition and immobilization of the amino-modified anti-aflatoxin DNA aptamer.…”

Section: Sensitivity Sensing Measurements and Discussionmentioning

confidence: 99%

“…To perform our sensing experiments, we flew several solutions containing different aflatoxin concentrations over the sensor devices. More details on the experimental process are available in [ 13 ]. Figure 9 represents the sensorgrams obtained for the L2 sample in TM polarization.…”

Section: Sensitivity Sensing Measurements and Discussionmentioning

confidence: 99%

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Design and Optimization of SiON Ring Resonator-Based Biosensors for Aflatoxin M1 Detection

Guider

Gandolfi

Chalyan

et al. 2015

Sensors

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In this article, we designed and studied silicon oxynitride (SiON) microring-based photonic structures for biosensing applications. We designed waveguides, directional couplers, and racetrack resonators in order to measure refractive index changes smaller than 10−6 refractive index units (RIU). We tested various samples with different SiON refractive indexes as well as the waveguide dimensions for selecting the sensor with the best performance. Propagation losses and bending losses have been measured on test structures, along with a complete characterization of the resonator’s performances. Sensitivities and limit of detection (LOD) were also measured using glucose-water solutions and compared with expected results from simulations. Finally, we functionalized the resonator and performed sensing experiments with Aflatoxin M1 (AFM1). We were able to detect the binding of aflatoxin for concentrations as low as 12.5 nm. The results open up the path for designing cost-effective biosensors for a fast and reliable sensitive analysis of AFM1 in milk.

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