Sensitive spectroscopic method for content analysis of cefixime in solid dosage form using hydrotropy phenomenon

Abstract

Aim:The main aim of this present investigation is to apply the hydrotropic solubilization phenomenon for spectroscopic analysis of poorly water-soluble drugs to avoid the use organic solvents which may be costlier, toxic to environment, volatile, and pollutant. Materials and Methods: A simple ultra violet spectroscopic method was used for the content analysis by diluting the drug cefixime with various hydrotropic agents. In this study, 20% solutions of sodium salicylate (SS), sodium citrate (SC), sodium acetate (SA), and sodium benzoate (SB) were used as hydrotropes for the analysis of cefixime. Results and Discussion: The drug cefixime showed the linearity of 0.5-2 µg/mL in SS, 5-30 µg/mL in SC, 5-50 µg/mL in SA, and 0.05-0.30 µg/mL in SB solution. Then the proposed methods were validated with respect to accuracy and precision as per International Conference of Harmonization guidelines Q2 (R1), November 2005 (Validation of Analytical Procedures: Text and Methodology). The drug showed less limit of detection (LOD) and limit of quantification (LOQ) values (LOD = 0.0225471 µg/mL and LOQ 0.0683246 µg/mL) to SB solution and it obeyed the Beer's law at very low concentration range (0.05-0.30 µg/mL) which proved that the drug has high sensitivity with SB solution. Conclusions: Finally, it was concluded that the all proposed methods were simple, cost-effective, safe to environment, rapid, reproducible, and highly sensitive with SB solution.

Validation of a Novel and Sensitive RP-HPLC Method for Simultaneous Determination of Cefixime Trihydrate and Sodium Benzoate in Powder for Oral Suspension Dosage Form

“…CFX is official in British Pharmacopeia (BP), European Pharmacopeia (EP) [1,2] and United States Pharmacopeia (USP) [8], both of them includes HPLC Method for its estimation. Literature review revealed that various analytical methods have been described for the determination of CFX including colorimetric and spectrophotometric [9][10][11][12][13][14][15][16][17][18][19][20][21][22], thin layer chromatography (TLC) [23][24][25][26], capillary electrophoresis [27][28][29], high performance liquid chromatography (HPLC) [30][31][32][33][34][35][36][37][38][39] and electrochemical methods [40][41][42][43] have been developed for the estimation of CFX in pure or in dosage forms. SDM is official in BP, EP [1,2] and United States Pharmacopeia (USP) [8], which includes direct titration for estimation of SDM.…”