Sensitive spectrofluorimetric determination of tizanidine in pharmaceutical preparations, human plasma and urine through derivatization with dansyl chloride

Abstract

A sensitive spectrofluorimetric method was developed for the determination of tizanidine in human plasma, urine and pharmaceutical preparations. The method is based on reaction of tizanidine with 1-dimethylaminonaphthalene-5-sulphonyl chloride (dansyl chloride) in an alkaline medium to form a highly fluorescent derivative that was measured at 511 nm after excitation at 383 nm. The different experimental parameters affecting the fluorescence intensity of tizanidine was carefully studied and optimized. The fluorescence-concentration plots were rectilinear over the ranges 50-500 and 20-300 ng/mL for plasma and urine, respectively, detection limits of 1.81 and 0.54 ng/mL and quantification limits of 5.43 and 1.62 ng/mL for plasma and urine, respectively. The method presents good performance in terms of linearity, detection and quantification limits, precision, accuracy and specificity. The proposed method was successfully applied for the determination of tizanidine in pharmaceutical preparations. The results obtained were compared with a reference method, using t- and F-tests.

A novel green spectrofluorimetric method for simultaneous determination of antazoline and tetryzoline in their ophthalmic formulation

“…Analysis of various primary, secondary amines, imidazoles, phenols, alcoholic hydroxyl groups and other several compounds was achieved using the fluorogenic reagent: DNS-Cl [33][34][35][36][37][38][39][40][41]. In the present study, ANT and TET were able to react with DNS-Cl yielding highly fluorescent yellow derivatives.…”

Selective spectrofluorimetric method for the determination of perindopril erbumine in bulk and tablets through derivatization with dansyl chloride

“…DNS has been used as a fluorogenic reagent for the determination of many pharmaceutical compounds (Aydoğmuş et al 2012;Abd El Ghaffar et al 2011;Abdel Fattah et al 2010;Ulu 2011;Karasakal and Ulu 2013). This paper describes, for the first time, the derivatization of PDE with dansyl chloride.…”

Development and validation of a reverse phase high performance liquid chromatography (HPLC) method for determination of tizanidine in human plasma

“…Momo et al (2010) determined tizanidine in human plasma and urine with use of liquid chroma-tography-tandem mass spectrometry in presence of maxiletine (Momo et al, 2010). Ulu et al (2012) utilized a spectroflourimetric method based on dervitization for estimation of tizanidine in plasma, urine and dosage forms (Ulu et al, 2012). …”