“…Rapid diagnosis of rotaviral infections is based on revealing the presence of rotaviral particles, their antigens or dsRNA in the clinical material samples at the early stage of infection. For its advantage, numerous methods were worked out, such as immunoenzymatic test using polyclonal and monoclonal antibodies (Dennehy et al, 1988;Pacini et al, 1988;Thomas et al, 1988;Brooks et al, 1989;Kok and Burrell 1989), agglutination using latex (Hughes et al, 1984), molecular hybridization (Arens and Swierkosz, 1989;Olive and Sethi, 1989), electrophoresis in polyacrylamide gel (Herring et al, 1982), the technique of direct electron microscopy as well as applying immunological aggregation of the virus (Benfield et al, 1984;Wu et al 1990) and recently RT-PCR technique (Gouvea et al, 1990;Wilde et al, 1990). Each of the above mentioned methods has its advantages and disadvantages.…”