Sensitive NADH detection in a tumorigenic cell line using a nano-biosensor based on the organic complex formation

Akhtar, Mahmood Hassan; Mir, Tanveer Ahmad; Gurudatt, N.G.; Chung, Saeromi; Shim, Yoon‐Bo

doi:10.1016/j.bios.2016.05.045

Cited by 23 publications

(15 citation statements)

References 17 publications

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“…Unlike biological in situ biosensors, assay conditions can be carefully controlled, allowing for calibration and absolute quantification of NADH. The detection limit for NADH in these devices is similar to those reported for MS/MS methods and in vivo fluorescent methods (~ 20–160 nM) [ 66 , 67 ].…”

Section: Introductionsupporting

confidence: 75%

Targeted redox and energy cofactor metabolomics in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum

Sander

Asano

Bhandari

et al. 2017

Biotechnol Biofuels

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Background Clostridium thermocellum and Thermoanaerobacterium saccharolyticum are prominent candidate biocatalysts that, together, can enable the direct biotic conversion of lignocellulosic biomass to ethanol. The imbalance and suboptimal turnover rates of redox cofactors are currently hindering engineering efforts to achieve higher bioproductivity in both organisms. Measuring relevant intracellular cofactor concentrations will help understand redox state of these cofactors and help identify a strategy to overcome these limitations; however, metabolomic determinations of these labile metabolites have historically proved challenging.ResultsThrough our validations, we verified the handling and storage stability of these metabolites, and verified extraction matrices and extraction solvent were not suppressing mass spectrometry signals. We recovered adenylate energy charge ratios (a main quality indicator) above 0.82 for all extractions. NADH/NAD+ values of 0.26 and 0.04 for an adhE-deficient strain of C. thermocellum and its parent, respectively, reflect the expected shift to a more reduced redox potential when a species lacks the ability to re-oxidize NADH by synthesizing ethanol. This method failed to yield reliable results with C. bescii and poor-growing strains of T. saccharolyticum. ConclusionsOur validated protocols demonstrate and validate the extraction and analysis of selected redox and energy-related metabolites from two candidate consolidated bioprocessing biocatalysts, C. thermocellum and T. saccharolyticum. This development and validation highlights the important, but often neglected, need to optimize and validate metabolomic protocols when adapting them to new cell or tissue types.

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Section: Introductionsupporting

confidence: 75%

Targeted redox and energy cofactor metabolomics in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum

Sander

Asano

Bhandari

et al. 2017

Biotechnol Biofuels

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“…NADH contains an adenine base and a NICO unit ( figure 15 a ). Shim and co-workers designed and constructed a sensor for complexing NADH via hydrogen bonds [ 85 ]. Ethylenediaminetetraacetic acid (EDTA) ( figure 15 b ) was covalently linked to a polyethylenimine (PEI)/activated graphene oxide composite by forming an amide bond between the NH 2 groups of PEI and the carboxylic acid groups of EDTA.…”

Section: Recognition Of Small Molecules By Polymer Materialsmentioning

confidence: 99%

Functional materials based on molecules with hydrogen-bonding ability: applications to drug co-crystals and polymer complexes

Hutchins¹

2018

R. Soc. open sci.

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The design, synthesis and property characterization of new functional materials has garnered interest in a variety of fields. Materials that are capable of recognizing and binding with small molecules have applications in sensing, sequestration, delivery and property modification. Specifically, recognition of pharmaceutical compounds is of interest in each of the aforementioned application areas. Numerous pharmaceutical compounds comprise functional groups that are capable of engaging in hydrogen-bonding interactions; thus, materials that are able to act as hydrogen-bond receptors are of significant interest for these applications. In this review, we highlight some crystalline and polymeric materials that recognize and engage in hydrogen-bonding interactions with pharmaceuticals or small biomolecules. Moreover, as pharmaceuticals often exhibit multiple hydrogen-bonding sites, many donor/acceptor molecules have been specifically designed to interact with the drug via such multiple-point hydrogen bonds. The formation of multiple hydrogen bonds not only increases the strength of the interaction but also affords unique hydrogen-bonded architectures.

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“…The best clinical, analytical, and technological performances are achieved by electrochemical sensors. The main biomarkers sensed by those instruments, are: VEGF165 [120,121], EGFR [122], Annexin II and MUC5AC [123], HIF-1α [124], NADH levels [125].…”

Section: Medical Clinical Applicationsmentioning

confidence: 99%

Emerging Designs of Electronic Devices in Biomedicine

et al. 2020

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A long-standing goal of nanoelectronics is the development of integrated systems to be used in medicine as sensor, therapeutic, or theranostic devices. In this review, we examine the phenomena of transport and the interaction between electro-active charges and the material at the nanoscale. We then demonstrate how these mechanisms can be exploited to design and fabricate devices for applications in biomedicine and bioengineering. Specifically, we present and discuss electrochemical devices based on the interaction between ions and conductive polymers, such as organic electrochemical transistors (OFETs), electrolyte gated field-effect transistors (FETs), fin field-effect transistor (FinFETs), tunnelling field-effect transistors (TFETs), electrochemical lab-on-chips (LOCs). For these systems, we comment on their use in medicine.

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Sensitive NADH detection in a tumorigenic cell line using a nano-biosensor based on the organic complex formation

Cited by 23 publications

References 17 publications

Targeted redox and energy cofactor metabolomics in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum

Targeted redox and energy cofactor metabolomics in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum

Functional materials based on molecules with hydrogen-bonding ability: applications to drug co-crystals and polymer complexes

Emerging Designs of Electronic Devices in Biomedicine

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