Sensitive high-performance liquid chromatographic method for the determination of the three main metabolites of selegiline (l-deprenyl) in human plasma

Croix, R. La; Pianezzola, E.; Benedetti, Μ. Strolin

doi:10.1016/0378-4347(94)00039-5

Cited by 21 publications

(7 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The original SPE procedure developed for the pretreatment of the samples (using C2 cartridges) allowed to obtain good purification of the biological samples and satisfactory precision in relatively shorter times using low volumes of solvents. The extraction yields (>97%) obtained with the proposed method are higher than those obtained by other authors who used liquid -liquid extraction [18,25,27,36,37], or SPE with C18 [16,28], Oasis HLB or MCX cartridges [14,38]. The method is very selective: none of the drugs tested caused any interference in the analysis.…”

Section: Discussioncontrasting

confidence: 54%

Determination of amphetamines in human urine by liquid chromatography with fluorimetric detection using a solid‐phase extraction procedure

Bugamelli

Mandrioli

Cavallini

et al. 2006

J of Separation Science

View full text Add to dashboard Cite

A precise and feasible HPLC method has been developed for the analysis of amphetamine (AMPH), methamphetamine (MAMPH) and methylenedioxymethamphetamine (MDMA, ecstasy) in human urine. A chromatographic run on a C8 Genesis (150 mm x 4.6 mm, 5 microm) column maintained at 30 degrees C lasts about 17 min, using a mobile phase composed of ACN (12%) and a pH 2.5 phosphate buffer (88%) containing 0.3% triethylamine. Mirtazapine was used as the internal standard. Good linearity was found in the 100-2000 ng/mL concentration range for AMPH and MAMPH and in the 12-2000 ng/mL concentration range for MDMA. The pretreatment of urine samples was carried out by means of a careful SPE procedure on C2 cartridges. The extraction yields were very satisfactory for all analytes, with average values greater than 97%. The leading conditions allowed the determination of AMPH, MAMPH and MDMA with satisfactory precision and accuracy. The method has been successfully applied to the determination of the analytes in urine of AMPH users.

show abstract

Section: Discussioncontrasting

confidence: 54%

Determination of amphetamines in human urine by liquid chromatography with fluorimetric detection using a solid‐phase extraction procedure

Bugamelli

Mandrioli

Cavallini

et al. 2006

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…In these methods, the reported LOD for AP and MP ranged from 1 to 100 ng/mL using plasma sample volume not less than 0.5 mL. La Croix et al (1994) reported an HPLC-FL method for the determination of AP and MP as metabolites of selegiline after derivatization with 9-fluorenylmethyl chloroformate. The authors did not report the LOD but reported 0.5 ng/mL as the quantitation limit of both AP and MP.…”

Section: Determination Of Ap and Mp In Abusers' Plasma And Hair Samplesmentioning

confidence: 98%

Determination of methamphetamine and amphetamine in abusers' plasma and hair samples with HPLC‐FL

Nakashima

Kaddoumi

Ishida

et al. 2003

Biomedical Chromatography

View full text Add to dashboard Cite

This paper describes highly sensitive HPLC methods for the determination of amphetamine (AP) and methamphetamine (MP) in abusers' plasma and hair samples. AP and MP were derivatized with the fluorescent reagent, DIB-Cl, to yield a highly fluorescent DIB-derivatives of AP and MP, which were then analyzed by HPLC with fluorescence detection at excitation and emission wavelengths of 325 and 430 nm, respectively. The separation was achieved on an ODS column with isocratic mobile phases composed of acetoniltrile and citrate buffer (55:45, v/v) for plasma samples and of acetonitrile-methanol-citrate buffer (45:20:37.5, v/v/v) for hair samples. The limits of detection were less than 0.87 ng/mL and 0.12 ng/mg in plasma and hair samples, respectively, for both AP and MP. The methods were then applied to the determination of MP and its metabolite AP in plasma obtained from two cases of illegally ingested MP and in one of the cases' hair received later. Case I was treated with dialysis; samples before and after dialysis were analyzed by the described method. After dialysis for 5 h, the total plasma levels of AP and MP decreased from 720 to 190 ng/mL. For case II, MP and AP levels were monitored for 3 days after digestion. Total plasma levels decreased from 57 ng/mL in the day of digestion to 11 ng/mL after 3 days. In hair samples, AP and MP could also be detected in very low concentrations.

show abstract

“…o-Phthaldialdehyde and 3-mercaptopropionic acid were used by Bowyer et al (1995) for determination of d-amphetamine in biological samples, the derivatives being detected by fluorescence. Also for fluorescence detection, amphetamine, methamphetamine and norselegiline present in human plasma have been derivatized with 9-fluorenylmethyl chloroformate (La Croix et al, 1994). On-line derivatization procedures with sodium 1,2-naphthoquinone-4-sulfonate, o-phthaldialdeyde, and 9-fluorenylmethyl chloroformate were performed by Herráez-Hernandez et al, (1996) for determination of amphetamine and methamphetamine in spiked urine and analysis by HPLC with diode array or fluorescence detection.…”

Section: Discussionmentioning

confidence: 99%

“…Derivatization procedures of amino groups with aplication in amphetamine analysis are described in literature, namely with 9-fluorenylmethyl chloroformate, phenyl isothiocyanate, o-phthaldialdehyde and 1-dimethylaminonaphthalene-5-sulfonyl chloride (dansylCl) as referred by La Croix et al, (1994). 1,2-naphthoquinone-4-sulfonate was also assayed (Herráez-Hernández et al, 1996) as well as 3-mercaptopropionic acid (Bowyer et al, 1995).…”

Section: Original Researchmentioning

confidence: 99%

Determination of amphetamine and its metabolite p‐hydroxyamphetamine in rat urine by reversed‐phase high‐performance liquid chromatography after dabsyl derivatization

Soares

Carvalho

Bastos

2001

Biomedical Chromatography

View full text Add to dashboard Cite

The consumption of amphetamine is illicit and controlled due to both the elicited behavioural deviations and the toxicity effects reported in abusers. Thus, amphetamine levels in biological samples must be monitored in several clinical and forensic circumstances. In spite of the interspecies differences in the preferred route of biotransformation, benzylmethylketone, benzoic acid and 4-hydroxyamphetamine are the principal metabolites of amphetamine. However, the clinical and forensic studies are focused in the parent compound and in 4-hydroxyamphetamine since benzylmethylketone is a minor metabolite in human and benzoic acid is also an endogenous compound. In the present study amphetamine and its metabolite, 4-hydroxyamphetamine, are quantified in urine by HPLC after derivatization with 4-dimethylaminoazobenzene-4'-sulfonyl chloride (dabsyl chloride). This derivatization procedure transforms amphetamine and its hydroxylated metabolite in compounds with similar lipofilicity, enabling their quantitative and simultaneous extraction with an organic solvent. The precision of the HPLC technique was 7.3 and 10.0% for amphetamine and 4-hydroxyamphetamine derivatives, respectively. For the overall procedure, including enzymatic hydrolysis, derivatization and extraction of the derivatives, the obtained values were 9.3 and 6.2%. Recoveries obtained from spiked urines for amphetamine and 4-hydroxyamphetamine were better than 97% and 94% (mean value), respectively. The detection limits of the method was 10 ng for both compounds. The principal advantages of the present proposed method are the stability of the dabsyl derivatives at room temperature and the detection carried out in the visible region, reducing the interferences detected.

show abstract

Sensitive high-performance liquid chromatographic method for the determination of the three main metabolites of selegiline (l-deprenyl) in human plasma

Cited by 21 publications

References 35 publications

Determination of amphetamines in human urine by liquid chromatography with fluorimetric detection using a solid‐phase extraction procedure

Determination of amphetamines in human urine by liquid chromatography with fluorimetric detection using a solid‐phase extraction procedure

Determination of methamphetamine and amphetamine in abusers' plasma and hair samples with HPLC‐FL

Determination of amphetamine and its metabolite p‐hydroxyamphetamine in rat urine by reversed‐phase high‐performance liquid chromatography after dabsyl derivatization

Contact Info

Product

Resources

About