Sensitive high-performance liquid chromatographic determination of 6-mercaptopurine, 6-thioguanine, 6-mercaptopurine riboside and 6-thioguanosine in biological fluids

Baal, John M. van; Leeuwen, M.B. van; Schouten, Teunis J.; Abreu, Ronney A. De

doi:10.1016/s0378-4347(00)85171-3

Cited by 21 publications

(1 citation statement)

References 11 publications

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“…Previously, v/e published HPLC methods for the detection of purine and pyrimidine nu cleotides [8], nucleosides and bases [9] and thiopurine nucleosides and bases [10,11], In the present article we describe two HPLC methods, one for measurement of methylthiopurine and thiopurine nucleosides and bases, and another for measurement of (methyl)thiopurine nu cleotides. Both methods are used in the clinical randomized ALL-8 trial of the Dutch Childhood Leukaemia Study Group.…”

Section: W Ken' En K Amp Jansen Ct At I J Ch Rom a Togr B 672mentioning

confidence: 99%

Determination of extracellular and intracellular thiopurines and methylthiopurines by high-performance liquid chromatography

Keuzenkamp-Jansen¹,

Abreu²,

Bökkerink³

et al. 1995

Journal of Chromatography B: Biomedical Sciences and Applicatio

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The thiopurine antimetabolites 6-thioguanine and 6-mercaptopurine are important chemotherapeutic drugs in the treatment of childhood acute lymphoblastic leukaemia. Measurement of metabolites of these thiopurines is important because correlations exist between levels of these metabolites and the prognosis in childhood acute lymphoblastic leukaemia. The reversed-phase method for the determination of extracellular thiopurine nucleosides and bases was previously developed and has been modified such that methylthiopurine nucleosides, bases, thioxanthine and thiouric acid can be measured also. The anion-exchange method enables the determination of intracellular mono-, di-and triphosphate (methyl)thiopurine nucleotides in one run. Extraction on ice with perchloric acid and dipotassium hydrogenphosphate results in good recoveries for (methyl)thiopurine nucleotides in lymphoblasts and peripheral mononuclear cells and for methylthioinosine nucleotides in red blood cells. Measure ment of the low concentrations of mono-, di-and triphosphate thioguanine nucleotides in red blood cells (detection limit 20 pmol/109 cells) is possible after extraction with methanol and methylene chloride, followed by oxidation of thioguanine nucleotides with permanganate and fluorimetric detection.

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Section: W Ken' En K Amp Jansen Ct At I J Ch Rom a Togr B 672mentioning

confidence: 99%

Determination of extracellular and intracellular thiopurines and methylthiopurines by high-performance liquid chromatography

Keuzenkamp-Jansen¹,

Abreu²,

Bökkerink³

et al. 1995

Journal of Chromatography B: Biomedical Sciences and Applicatio

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Highly‐Sensitive Determination of 6‐Mercaptopurine and Its Metabolites by Electrochemical Reductive Desorption Measurements

et al. 2005

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The electrochemical detection of 6-mercaputopurine (6-MP), which is one of the most well-known drugs of antimetabolites, and its analogues was studied. The presented method is based on chemisorption (accumulation)/ desorption processes of drugs onto/from the Au electrode surface. Using this method, we succeeded in detecting 6-MP even in very diluted solution (concentration of 6-MP: 1 Â 10 À8 M). The lower detection limit of 6-MP was considerably improved compared with the previous HPLC studies by using our proposed system. Desorptive potentials of 6-MPmetabolites and its analogues were different from those of 6-MP. This provides the possibility of simultaneous detection of 6-MP and its typical metabolites.

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Application of the iodine–azide postcolumn reaction in RP‐HPLC for the determination of thioguanine in urine

Zakrzewski

2008

J of Separation Science

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The study focuses on the analysis of a sensitive, selective, and simple postcolumn detection method for thioguanine determination, based on the sensitizing induction of thioguanine on iodine-azide reaction and the combination technique of HPLC. The analysis was accomplished in the optimum conditions for iodine-azide detection system and HPLC separation. The values for the linear range, the LOD, and DOQ amounted to 0.8-1.7, 0.4, and 0.5 nmol/mL urine, respectively.

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Sensitive high-performance liquid chromatographic determination of 6-mercaptopurine, 6-thioguanine, 6-mercaptopurine riboside and 6-thioguanosine in biological fluids

Cited by 21 publications

References 11 publications

Determination of extracellular and intracellular thiopurines and methylthiopurines by high-performance liquid chromatography

Determination of extracellular and intracellular thiopurines and methylthiopurines by high-performance liquid chromatography

Highly‐Sensitive Determination of 6‐Mercaptopurine and Its Metabolites by Electrochemical Reductive Desorption Measurements

Application of the iodine–azide postcolumn reaction in RP‐HPLC for the determination of thioguanine in urine

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