Highly‐Sensitive Determination of 6‐Mercaptopurine and Its Metabolites by Electrochemical Reductive Desorption Measurements

Abstract

The electrochemical detection of 6-mercaputopurine (6-MP), which is one of the most well-known drugs of antimetabolites, and its analogues was studied. The presented method is based on chemisorption (accumulation)/ desorption processes of drugs onto/from the Au electrode surface. Using this method, we succeeded in detecting 6-MP even in very diluted solution (concentration of 6-MP: 1 Â 10 À8 M). The lower detection limit of 6-MP was considerably improved compared with the previous HPLC studies by using our proposed system. Desorptive potentials of 6-MPmetabolites and its analogues were different from those of 6-MP. This provides the possibility of simultaneous detection of 6-MP and its typical metabolites.

Fluorescence analysis of 6-mercaptopurine with the use of a nano-composite consisting of BSA-capped Au nano-clusters and core–shell Fe3O4–SiO2 nanoparticles

“…Consequently, it is important to monitor the concentrations of the 6-MP frequently and hence, the methods of analysis should be not only suitable for purpose, but also be inexpensive and easy to apply. At present, there are several methods of analysis of 6-MP, e.g., traditional fluorescence (FL) (Wang and Zhang, 2008), chemiluminescence (CL), (Yang et al, 2008), electrochemical (EC) (Sato et al, 2005;Lu et al, 2008), high performance liquid chromatography (HPLC) (Su et al, 1999), and fluorescence analysis based on quantum dots (Chen et al, 2013;Gao et al, 2013). However, these methods have some limitations including, e.g., reagents, time-consuming processes, relatively low sensitivity, and poor selectivity.…”

Development of Highly-sensitive Electrochemical Biosensors

“…Actually, 6-mercaputopurin [9], a drug of antimetabolite, and acethylcholinesterase (AChE) [25,26] that produced thiocholine through the hydrolysis of acethythiocholine, could be determined with the detection limits of 10 nM and 10 mU/L, respectively. The procedure for measuring the AChE activity has successfully been applied to immunoassay systems by employing AChE as a marker enzyme: BNP from 15 to 150 pM could be determined within an hour [12,27].…”

A new fluorescent nitrogen-doped carbon dot system modified by the fluorophore-labeled ssDNA for the analysis of 6-mercaptopurine and Hg (II)

“…Consequently, it is important to monitor the concentrations of 6-MP in human serum. Several methods, such as traditional fluorescence (FL) (Wang and Zhang, 2008), chemiluminescence (CL) (Yang et al, 2008), electrochemical (EC) (Sato et al, 2005;Lu et al, 2008), high performance liquid chromatography (HPLC) (Su et al, 1999), and fluorescence analysis based on quantum dots (Chen et al, 2013;Gao et al, 2013) have been used to analyse 6-MP.…”