Sensitive determination of metalloprotein in salt-rich matrices by size exclusion chromatography coupled with inductively coupled plasma-mass spectrometry

Endocytosis-Mediated Transport of Pb in Rat Blood Cells

“…Size exclusion chromatography (SEC) was used for the separation and detection of Pb-binding proteins by coupling with ICP-MS. The details of extracted and detected proteins were described in a previous study . Briefly, the total proteins were extracted by a protein lysis solution.…”

“…The details of extracted and detected proteins were described in a previous study. 49 Briefly, the total proteins were extracted by a protein lysis solution. After ultrafiltration, the protein was preseparated in high-performance liquid chromatography (HPLC, Agilent 1200 HPLC Pump, Agilent Technologies Inc., Germany), separated on a gel column (TSK-GEL G3000 SWXL columns (7.8 mm × 30 cm dimension, 5 μm particle size, 250 °A pore size) and a guard column TSK Guard SWXL 6.0 mm × 40 mm (Shiba, Minato-Ku, Tokyo, Japan)) and detected by ICP-MS to obtain the signal peaks of Pb-binding protein.…”

Atomic spectrometry update: review of advances in elemental speciation

“…A recently published indirect method for the analysis of Pb containing proteins has utilised labelling strategies for the analysis of biological fluids from bone marrow . 135 The method for the separation and detection of proteins by using SEC-ICP-MS has used a commercially available High Matrix Introduction (HMI) system to overcome perceived issues around eluents containing high levels of buffer-salts. The HMI technology uses an aerosol dilution approach with clean, dry argon gas to reduce the loading on the plasma, which is reported to allow the plasma to tolerate much higher total dissolved solids levels than the 0.1 to 0.2% that is typical for ICP-MS.…”

Rebuttal to Correspondence on “Endocytosis-Mediated Transport of Pb in Rat Blood Cells”

“…The study found that the detection limit of Pb was 0.0089 μg L −1 , which is similar to the detection limit under the standard mode (0.0013 μg L −1 ). 12 In addition, we clarify that validating proteins with antibodies is not an optimal option. It is important to note that protein-specific antibodies are designed on the basis of fragments of the protein's primary structure.…”

“…With regard to methodology, we published a series of papers on both analysis of metalloproteins and functions of metals in various organisms, which prove the validity and feasibility of our methods. − We chose sample preparation and analysis conditions different from those of typical chemical analysis can be attributed to being in line with the typical conditions used in the biochemical and biological study of proteins. In our previous research, ammonium nitrate has also been used as a mobile phase for separation .…”