Sensitive determination of low molecular mass phenols by liquid chromatography with chemiluminescence detection for the determination of phenol and 4-methylphenol in urine

Orejuela, Eva; Silva, Manuel

doi:10.1039/b207321h

Cited by 26 publications

(12 citation statements)

References 48 publications

(60 reference statements)

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“…The detection limits achieved by this method were considerably lower than with other methodologies. 92 The peroxyoxalate system has been utilized to quantify the amount of trans-resveratrol bound to a molecular imprinted polymer, consisting of uniform trans-resveratrol coated microspheres, with poly(vinyl alcohol) as glue. The low detection limit of 0.1 µg mL -1 , indicates that this system can be used to analyze trans-resveratrol in various samples.…”

Section: Analytical Applications Of Peroxyoxalate Chemiluminescencementioning

confidence: 99%

The chemiluminescent peroxyoxalate system: state of the art almost 50 years from its discovery

Francisco¹,

Ciscato²,

Augusto³

et al. 2012

Arkivoc

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Almost fifty years after the discovery of the peroxyoxalate reaction by E. A. Chandross in the early nineteen sixties, this review article intends to give a general overview on mechanistic aspects of this system and to describe the principles of its analytical application. After a short general introduction on the principles of chemiluminescence and the history of peroxyoxalate discovery, mechanistic aspects of high-energy intermediate formation, its structure and its reaction with an activator in the peroxyoxalate system are discussed. Finally, analytical applications of peroxyoxalate chemiluminescence are exemplified using representative recent examples, including oxalic acid detection in biological samples.

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Section: Analytical Applications Of Peroxyoxalate Chemiluminescencementioning

confidence: 99%

The chemiluminescent peroxyoxalate system: state of the art almost 50 years from its discovery

Francisco¹,

Ciscato²,

Augusto³

et al. 2012

Arkivoc

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“…Phenol was the least potent chemical tested in this study with LOAELs in the millimolar range; however, it was very effective at these high doses. Phenol (5.7 lg/ml) and 4-methylphenol (1.78 lg/ml) have been detected in the urine of smokers (Orejuela and Silva, 2002). Taken together, the data suggest that phenol, if present in cigarette smoke in high enough concentration, could enter the circulatory system and inhibit oviductal functioning, especially oocyte pickup rate and smooth muscle contraction rate, effectively.…”

mentioning

confidence: 81%

Phenols, Quinolines, Indoles, Benzene, and 2-Cyclopenten-1-ones are Oviductal Toxicants in Cigarette Smoke

Riveles¹,

Roza²,

Talbot³

2005

Toxicological Sciences

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Previously, we showed that pyridines and pyrazines in cigarette smoke inhibit oviductal functioning in vitro in nanomolar and picomolar doses. The purpose of this study was to determine the lowest observable adverse effect levels (LOAELs) of phenols, quinolines, indoles, benzene, and 2-cyclopenten-1-ones found in mainstream smoke solutions on ciliary beat frequency, oocyte pickup rate, and infundibular smooth muscle contraction using the hamster oviduct. After solid phase extraction, mainstream smoke solution fractions were tested in the oviductal assays. The active fractions were analyzed using gas chromatography-mass spectrometry to identify individual chemicals. Using this approach, benzene, eleven phenolic, two indole, two quinoline, and two 2-cyclopenten-1-one derivatives were identified in the active fractions. Commercially available authentic standards of the identified compounds were tested in dose-response studies on hamster oviducts. The LOAELs were determined for each compound using the ciliary beat frequency, oocyte pickup rate, and infundibular smooth muscle contraction rate assays. Indole, the compound with the highest potency, showed inhibition of ciliary beat frequency (10(-13) M), oocyte pickup rate (10(-14) M), and infundibular smooth muscle contraction rate (10(-15) M) in femtomolar doses. All of the other compounds tested, except phenol, which only showed inhibition at millimolar concentrations, were inhibitory in picomolar, nanomolar, or micromolar concentrations. Derivitization of phenol increased its toxicity in the oviductal assays, especially when a methyl or ethyl group was substituted on the fourth position. The indoles, quinolines, and four phenolic compounds had both high potencies and efficacies in the oviductal assays.

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“…Low molecular mass phenols, such as phenol and 4-methylphenol, in urine samples have been quantified by means of HPLC-PO-CL [55]. Urine samples were cleaned up and phenols were extracted by SPE (LiChrolut EN sorbent column) and then simply derivatized with dansyl chloride (15 min.…”

Section: Determination Of Phenolsmentioning

confidence: 99%

Chemiluminescence detection in liquid chromatography: Applications to clinical, pharmaceutical, environmental and food analysis—A review

Gámiz‐Gracia

Garcı́a-Campaña

Huertas-Pérez

et al. 2009

Analytica Chimica Acta

161

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Sensitive determination of low molecular mass phenols by liquid chromatography with chemiluminescence detection for the determination of phenol and 4-methylphenol in urine

Cited by 26 publications

References 48 publications

The chemiluminescent peroxyoxalate system: state of the art almost 50 years from its discovery

The chemiluminescent peroxyoxalate system: state of the art almost 50 years from its discovery

Phenols, Quinolines, Indoles, Benzene, and 2-Cyclopenten-1-ones are Oviductal Toxicants in Cigarette Smoke

Chemiluminescence detection in liquid chromatography: Applications to clinical, pharmaceutical, environmental and food analysis—A review

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