Sensitive determination of clarithromycin in human plasma by high-performance liquid chromatography with spectrophotometric detection

Pharmacokinetics of clarithromycin after single intravenous and intracrop bolus administrations to broiler chickens

“…The plasma samples were then placed in polyurethane Eppendorf tubes in which they are kept at -50 °C until analysed by high performance liquid chromatography (HPLC). The analytical HPLC procedure for determination of clarithromycin in plasma was adopted according to the method presented by (Amini and Ahmadiani 2005). Twenty μl of 1 N NaOH were added to 1 mL of plasma.…”

Simultaneous determination of clarithromycin, rifampicin and their main metabolites in human plasma by liquid chromatography–tandem mass spectrometry

“…Chromatographic methods with fluorescent, ultraviolet, electrochemical or amperometric detection of the individual drugs [7][8][9][10][11][12][13][14][15] are time consuming, mostly require large sample volumes and are less sensitive than methods with mass spectrometric detection. The reported chromatographic methods for clarithromycin with tandem mass spectrometric detection (LC/MS/MS) [16][17][18][19] achieve sufficient sensitivities in short run times, but suffered from lacking the active 14-hydroxymetabolite.…”

Determination of clarithromycin in human serum by high-performance liquid chromatography after pre-column derivatization with 9-fluorenylmethyl chloroformate: Application to a bioequivalence study

“…Due to weak UV absorbance of macrolide antibiotics, it is difficult to develop a simple method for analysis of the drugs in biological fluids using conventional UV detection, thus, high-performance liquid chromatography (HPLC) with electrochemical detection (ED) is the standard method for analysis of the drugs in biological matrix. Several HPLC methods have been reported for analysis of clarithromycin in human serum using EC [2][3][4][5][6][7][8][9], MS [10], UV [11] and fluorescence detections [12]. Determination of the drug in serum samples using HPLC with UV detection at 205 nm has been recently reported by Amini and Ahmadiani [11] and although, limit of quantification (LOQ) of 31.25 ng mL −1 has been obtained using injection volume of 100 L, however, due to weak UV absorbance of the drug the system should be set at highest sensitivity.…”