Sensitive Detection of SARS-CoV-2 Using a Novel Plasmonic Fiber Optic Biosensor Design

Saad, Yosra; Gazzah, Mohamed Hichem; Mougin, Karine; Selmi, Marwa; Belmabrouk, Hafedh

doi:10.1007/s11468-022-01639-2

Cited by 18 publications

(6 citation statements)

References 63 publications

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“…Biosensors for the direct detection of SARS-CoV-2 under point-of-care conditions can represent a wide group of analytical devices using immunochemical or molecular biology approaches to identify the unique determinants distinguishing SARS-CoV-2 from other viruses or biological materials. In addition to biosensors for use in point-of-care conditions, there are also highly sophisticated biosensors suitable for SARS-CoV-2 detection [ 100 , 101 , 102 , 103 , 104 , 105 ]. In this review, portable platforms ready to be used in the conditions of home care or other outside laboratory environments are taken into account.…”

Section: Biosensors For Measuring Sars-cov-2 and Its Determining Partsmentioning

confidence: 99%

Progress in Biosensors for the Point-of-Care Diagnosis of COVID-19

Pohanka

2022

Sensors

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Coronavirus disease 2019 (COVID-19) is a highly virulent infection that has caused a pandemic since 2019. Early diagnosis of the disease has been recognized as one of the important approaches to minimize the pathological impact and spread of infection. Point-of-care tests proved to be substantial analytical tools, and especially lateral flow immunoassays (lateral flow tests) serve the purpose. In the last few years, biosensors have gained popularity. These are simple but highly sensitive and accurate analytical devices composed from a selective molecule such as an antibody or antigen and a sensor platform. Biosensors would be an advanced alternative to current point-of-care tests for COVID-19 diagnosis and standard laboratory methods as well. Recent discoveries related to point-of-care diagnostic tests for COVID-19, the development of biosensors for specific antibodies and specific virus parts or their genetic information are reviewed.

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Section: Biosensors For Measuring Sars-cov-2 and Its Determining Partsmentioning

confidence: 99%

Progress in Biosensors for the Point-of-Care Diagnosis of COVID-19

Pohanka

2022

Sensors

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“…License: CC BY-NC-ND 4.0 such as MXenes 10 , metasurfaces 11 , a new background subtraction strategy 12 , and the use of plasmonics for the detection of target biomarkers in saliva and diluted whole blood 13 . Furthermore, the COVID-19 pandemic enabled the validation of portable sensing platforms such as fiber-optics-based SPR sensors 14 , smartphone-based LSPR detection synchronized with machine learning (ML) 15 , and lightweight SPR sensors with simple graphical user interface [16][17][18] . As such, SPR sensors were developed to measure antibody titer 17,19 , combined antibody affinity and titer measurements 18,20,21 , and viral proteins 22,23 .…”

Section: Introductionmentioning

confidence: 99%

Large-scale validation of a plasmonic sensor for SARS-CoV-2 pseudo-neutralization with a cohort of food and retail workers

Coutu,

Ricard,

Djaïleb

et al. 2023

Preprint

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Plasmonic sensors are candidates for numerous clinical applications, but few examples demonstrate their performance on large sample cohorts, a necessary step for clinical translation. The COVID-19 pandemic provided an unprecedented opportunity to validate a surface plasmon resonance (SPR) sensor for SARS-CoV-2 inhibition with a cohort of over 1,000 clinical samples from the longitudinal study of a food and retail worker population. The SPR sensor provided an in vitro model to assess the level of neutralizing antibodies by measuring the inhibition of the SARS-CoV-2 spike protein interaction with ACE-2 following exposure of the spike protein to naive and immune sera (from vaccination and/or infection). In conjunction with population data on vaccination and infection, and epidemiological data from the local jurisdiction of the study cohort, it is shown that the SPR sensor performed well in assessing the level of “pseudo-neutralization” of participant sera and that the response of the SPR sensor correlates (r = 0.74) with a live virus microneutralization assay as well as with metadata of relevant events (vaccination, waves of infection, etc.) that occurred during the study period. Using these data, the article details the challenges and opportunities of using plasmonic sensors in clinical practice.

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“…Colorimetric biosensors using gold nanoparticles (AuNPs) are easy to use, and the detection is through the visualization of a color change resulting from plasmonic interactions between the particles [ 11 ]. AuNPs are the preferred metal in most SPR sensors owing to their unique optical properties and biocompatibility [ 12 ]. Therefore, these reactions are promising for diagnosing various infectious diseases [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…The functionalization of the surface significantly affects the performance of AuNP-based colorimetry. The immobilization of the ligand on the surface leads to a negative effect on molecular interactions [ 12 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle-Based Visual Detection of Amplified DNA for Diagnosis of Hepatitis C Virus

Kim

et al. 2022

Biosensors

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Rapid, simple, and inexpensive diagnostic point-of-care tests (POCTs) are essential for controlling infectious diseases in resource-limited settings. In this study, we developed a new detection system based on nanoparticle–DNA aggregation (STat aggregation of tagged DNA, STAT-DNA) to yield a visual change that can be easily detected by the naked eye. This simplified optical detection system was applied to detect hepatitis C virus (HCV). Reverse transcription-polymerase chain reaction (RT-PCR) was performed using primers labeled with biotin and digoxigenin. Streptavidin-coated magnetic particles (1 μm) and anti-digoxigenin antibody-coated polystyrene particles (250–350 nm) were added to form aggregates. The limit of detection (LoD) and analytical specificity were analyzed. The STAT-DNA results were compared with those of the standard real-time PCR assay using serum samples from 54 patients with hepatitis C. We achieved visualization of amplified DNA with the naked eye by adding nanoparticles to the PCR mixture without employing centrifugal force, probe addition, incubation, or dilution. The LoD of STAT-DNA was at least 101 IU/mL. STAT-DNA did not show cross-reactivity with eight viral pathogens. The detection using STAT-DNA was consistent with that using standard real-time PCR.

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Sensitive Detection of SARS-CoV-2 Using a Novel Plasmonic Fiber Optic Biosensor Design

Cited by 18 publications

References 63 publications

Progress in Biosensors for the Point-of-Care Diagnosis of COVID-19

Progress in Biosensors for the Point-of-Care Diagnosis of COVID-19

Large-scale validation of a plasmonic sensor for SARS-CoV-2 pseudo-neutralization with a cohort of food and retail workers

Nanoparticle-Based Visual Detection of Amplified DNA for Diagnosis of Hepatitis C Virus

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