Sensitive detection of glutathione by using DNA-templated copper nanoparticles as electrochemical reporters

Wang, Zhuxin; Han, Peng; Mao, Xiaoxia; Yin, Yuan; Cao, Ya

doi:10.1016/j.snb.2016.07.078

Cited by 43 publications

(11 citation statements)

References 37 publications

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“…This strategy has been combined with the use of aptamers for the detection of PSA biomarker at fg/mL levels [62]. The strong interaction between glutathione (GSH) and copper ions [63] has also been approached for the detection of GSH after formation of DNA-templated CuNPs and later voltammetric detection, as described above [64]. This analytical signal readout has also been used in the development of a method for quantifying endonuclease activity [65].…”

Section: Nanoparticles As Electroactive Labelsmentioning

confidence: 99%

Nanoparticles as Emerging Labels in Electrochemical Immunosensors

Iglesias-Mayor

Amor-Gutiérrez

Costa-Garcı́a

et al. 2019

Sensors

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This review shows recent trends in the use of nanoparticles as labels for electrochemical immunosensing applications. Some general considerations on the principles of both the direct detection based on redox properties and indirect detection through electrocatalytic properties, before focusing on the applications for mainly proteins detection, are given. Emerging use as blocking tags in nanochannels-based immunosensing systems is also covered in this review. Finally, aspects related to the analytical performance of the developed devices together with prospects for future improvements and applications are discussed.

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Section: Nanoparticles As Electroactive Labelsmentioning

confidence: 99%

Nanoparticles as Emerging Labels in Electrochemical Immunosensors

Iglesias-Mayor

Amor-Gutiérrez

Costa-Garcı́a

et al. 2019

Sensors

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“…Low sensitivity and necessity of derivation are the other disadvantages that account for the reduced use of these methods. In contrast, electrocatalytic methods provide such benefits as simplicity, inexpensiveness, high sensitivity, reproducibility, and easy miniaturization [15].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical determination of glutathione in hemolysed erythrocytes

2020

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The physiological significance of determining glutathione (GSH) and its oxide form is obvious from their applications in clinical practices such as diagnostic experiments for diabetes, Parkinson's disease, and cancers. Such an important detemination still needs the development of certain experimental procedures that are easy, fast, and cheap enough to implement. These procedural advantages can be provided through electrochemical methods. Therefore, in this study, at the surface of a glassy carbon electrode (GCE), a composite of functionalized multi-walled carbon nanotubes (MWCNTs) and formazon was used as a mediator to determine GSH electrochemically. The results indicated that this modified GCE is electrocatalytically very active for glutathione oxidation. Several techniques including cyclic voltammetry (CV), scanning electron microscopy, and differential pulse voltammetry (DPV) were used to characterize the electrode. Also, such kinetic parameters as the charge transfer rate constant and the transfer coefficient were calculated. In optimized conditions, there was a linear relationships between the DPV peak current of GSH oxidation and GSH concentration in the ranges of 1.0-100.0 and 100.0-800.0 µM at pH 7.0. As for the detection limit, it was found to be 0.73 µM. Once put to practice, the devised method proved to be capable of measuring GSH in blood samples.

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“…Electrochemical methods for CRp determination have been well accepted as an alternative to the previously mentioned methods. Electrochemical methods are highly sensitive, produce results in comparatively shorter times, and require lower costs [12]. However, to the best of our knowledge, there have not been any published articles in the specialized literature dealing with CRp generation by Solanum betaceum , also known as “tomato tree” in Ecuador, other South American countries, and some parts of Europe.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Cadmium Accumulation in Tamarillo Cells (Solanum betaceum) by Indirect Electrochemical Detection of Cysteine-Rich Peptides

et al. 2019

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Long-term cadmium intake can be very dangerous to human health due to its toxic effects. Although people can be contaminated with this element from different sources, contaminated food is probably the most important one. Foods such as vegetables and fruits can become contaminated with cadmium existing in soils, irrigation water, or chemical fertilizers. Some plants produce an excess of cysteine-rich peptides (CRp) when affected by high concentrations of heavy metals such as cadmium, thus indicating the presence of this type of contamination. Among these plants is tamarillo (Solanum betaceum), which is locally known as “tree tomato”. This is a native plant widely consumed in the Ecuadorian Andes because of its abundance, low cost, and high content of vitamin C and fiber. The fact that Solanum betaceum produces CRp upon contamination with heavy metals means that this plant may be able to accumulate heavy metals. If this is the case, the plant can possibly be used as an indicator of metal pollution. The main goals of the present work were to evaluate the possibility of using Solanum betaceum as an indicator of metal contamination in plants and to examine its capability to accumulate metals. Both goals were met by determination of the amounts of CRp produced by Solanum betaceum cells cultivated in vitro in the laboratory under controlled conditions in the presence of different concentrations of cadmium. The CRp determination was carried out by means of electrogeneration of iodine in an iodide solution containing reduced glutathione as a biological thiol model. Solanum betaceum cells were grown in a Murashige and Skoog solution enriched with a 30 g L−1 sugar aqueous solution and 1 mg L−1 2,4-dichlorophenoxyacetic acid. The results of these experiments confirmed the following: (1) CRp production is a function of the amount of cadmium present as a contaminant up to a limiting value after which cell apoptosis occurs; (2) Solanum betaceum accumulates cadmium; (3) the analytical method used is appropriate for CRp determination; and (4) CRp determination is a valid alternative to detect contamination by heavy metals in plants.

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Sensitive detection of glutathione by using DNA-templated copper nanoparticles as electrochemical reporters

Cited by 43 publications

References 37 publications

Nanoparticles as Emerging Labels in Electrochemical Immunosensors

Nanoparticles as Emerging Labels in Electrochemical Immunosensors

Electrochemical determination of glutathione in hemolysed erythrocytes

Evaluation of the Cadmium Accumulation in Tamarillo Cells (Solanum betaceum) by Indirect Electrochemical Detection of Cysteine-Rich Peptides

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