Sensitive detection of copper(<scp>ii</scp>) ions based on the conformational change of peptides by surface plasmon resonance spectroscopy

Chen, Hongxia; Jia, Shengsong; Zhang, Jiangjiang; Jang, Minji; Chen, Xiuying; Koh, Kwangnak; Wang, Zhaoxia

doi:10.1039/c5ay02047f

Cited by 23 publications

(19 citation statements)

References 47 publications

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“…A wide range of methods including atomic absorption spectroscopy, ion‐selective electrodes, inductively coupled plasma mass spectroscopy, inductively coupled atomic emission spectroscopy, voltammetry and X‐ray fluorescence, have been used for the detection of copper . Even though these methods provide a good level of detection over a wide range of concentration range, most of them require expensive instruments, well‐trained personnel and thorough sample preparation.…”

Section: Introductionmentioning

confidence: 99%

Highly Selective and Sensitive (PPB Level) Quinolin‐Based Colorimetric Chemosensor for Cu(II)

et al. 2020

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4-(quinolin-2-ylmethylene)aminophenol (QMAP) is synthesised in a one-step synthetic process and was checked for its sensing ability towards metal ions. The molecule is found to be effective for the instantaneous selective and sensitive Cu(II) ion detection in PPB level in methanolic solution with a distinct development of orangish yellow colour from a colourless solution, which can be detected visually. The sensing ability of the QMAP towards Cu(II) is found independent on the type of counter anions and also in the presence of other metal ions. QMAP does not show any colour change in the presence of other metal salts. A 2 : 1 binding stoichiometry between QMAP and Cu(II) with a large binding constant of 3.2 x 10 10 M À 2 has been measured. Quantum chemical calculations have been used to understand the nature of the complex and the mechanism of the sensing.

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Section: Introductionmentioning

confidence: 99%

Highly Selective and Sensitive (PPB Level) Quinolin‐Based Colorimetric Chemosensor for Cu(II)

et al. 2020

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“…Therefore, the application of molecular detection technology has been widely studied. Other peptide-based SPR biosensors have been used to detect heavy metals,27,28 the real-time detection of breast cancer,29 staphylococcal enterotoxin B,30 and peptide-Au nanorods to detect cardiac biomarkers 31. In addition, the peptide-graphene based combination has uniquely excellent properties for use in non-immunological biosensors,32,33 antimicrobial,34 and drug carriers 35.…”

Section: Introductionmentioning

confidence: 99%

<p>High-affinity carboxyl-graphene oxide-based SPR aptasensor for the detection of hCG protein in clinical serum samples</p>

Chiu¹,

Kuo²,

Chen³

2019

IJN

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Background The use of functionalized graphene oxide (fGO) has led to a new trend in the sensor field, owing to its high sensitivity with regards to sensing characteristics and easy synthesis procedures. Methods In this study, we developed an ultra-sensitive carboxyl-graphene oxide (carboxyl-GO)-based surface plasmon resonance (SPR) aptasensor using peptides to detect human chorionic gonadotropin (hCG) in clinical serum samples. The carboxyl-GO based SPR aptasensor provided high affinity and stronger binding of peptides, which are great importance to allow for a non-immunological label-free mechanism. Also, it allows the detection of low concentrations of hCG, which are in turn considered to be important clinical parameters to diagnose ectopic pregnancies and paraneoplastic syndromes. Results The high selectivity of the carboxyl-GO-based SPR aptasensor for hCG recombinant protein was verified by the addition of the interfering proteins bovine serum albumin (BSA) and human serum albumin (HSA), which did not affect the sensitivity of the sensor. The carboxyl-GO-based chip can enhance the assay efficacy of interactions between peptides and had a high affinity binding for a k a of 17×10 6 M −1 S −1 . The limit of detection for hCG in clinical serum samples was 1.15 pg/mL Conclusion The results of this study demonstrated that the carboxyl-GO-based SPR aptasensor had excellent sensitivity, affinity and selectivity, and thus the potential to be used as disease-related biomarker assay to allow for an early diagnosis, and possibly a new area in the field of biochemical sensing technology.

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“…26,30 An area of growing interest is the design of copper sensing probes that employ lanthanides, namely Tb(III) and Eu(III), due to their highly attractive photophysical properties (e.g., millisecond luminescence lifetimes, large Stokes shift, emission in the visible and sharp distinct emission bands, high photostability and low phototoxicity). 32,33 Designs of lanthanide probes for copper sensing include small molecule probes, [17][18][19][20][21][22][23][24][25][26][27] peptide mimics of metallo-protein binding sites, 45 nanoparticles, 8 and polymer-based materials. [34][35][36][37][38][39][40][41][42][43][44] The most typical design for small molecule copper sensors based on lanthanides are compounds that contain sensitizer-receptor units, as for example seen in the sensor S1 reported by Gunnlaugsson et al (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

A Luminescent NOTA-Based Terbium(III) “Turn-Off” Sensor for Copper

et al. 2019

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A bimacrocyclic luminescent terbium(III) sensor is reported for the selective 'turn-off' detection of Cu 2+ ions in aqueous solutions. The current sensor differentiates from previous sensors in that it offers the use of 1) time-gated luminescence detection to remove background signal, 2) a longer excitation wavelength of up to 350 nm for increased biocompatibility, and 3) a practically irreversible detection as a form of probing Cu 2+ ions with an extremely low limit of detection of about 1.7 nM. Synopsis: The detection of copper ions in aqueous solutions is an active and growing research field relevant to bioanalyses and environmental sampling. There is a high demand for sensitive and selective probes for copper in biological environment including intracellular monitoring. This would allow for a better understanding of the bivalent role of copper in the human body. Herein, we report a bimacrocyclic luminescent terbium(III) sensor for the selective 'turn-off' detection of Cu 2+ ions in aqueous solutions.

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Sensitive detection of copper(ii) ions based on the conformational change of peptides by surface plasmon resonance spectroscopy

Abstract: A novel quantitative copper (ii) ion sensor is developed based on the conformational change of Cu2+ binding peptides using surface plasmon resonance (SPR) spectroscopy.

Cited by 23 publications

References 47 publications

Highly Selective and Sensitive (PPB Level) Quinolin‐Based Colorimetric Chemosensor for Cu(II)

Highly Selective and Sensitive (PPB Level) Quinolin‐Based Colorimetric Chemosensor for Cu(II)

<p>High-affinity carboxyl-graphene oxide-based SPR aptasensor for the detection of hCG protein in clinical serum samples</p>

A Luminescent NOTA-Based Terbium(III) “Turn-Off” Sensor for Copper

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