2016
DOI: 10.1039/c6an01087c
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Sensitive detection of acid phosphatase based on graphene quantum dots nanoassembly

Abstract: In this study, we report a convenient label-free fluorescence biosensor for the detection of acid phosphatase based on the aggregation-caused quenching of graphene quantum dots (GQDs). The fluorescence of GQDs could be quenched by poly-(dimethyl diallyl ammonium chloride) (PDDA); the high efficiency of the quenching was caused by the non-covalent binding of positively charged PDDA to negatively charged GQDs through electrostatic interactions, aggregating to form a GQDs-PDDA complex. Addition of sodium hexameta… Show more

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Cited by 18 publications
(1 citation statement)
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References 24 publications
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“…A significant linear correlation ( R 2 = 0.989) existed between the [log ( F – F 0 )] and the ACP concentration in the range of 1.0–50 mU/L. The limit of detection (LOD) was estimated to be 0.3 mU/L, which was lower than some fluorescence methods in Table . In addition, Figure illustrated that the established method displayed ascendant selectivity for ACP sensing. Interestingly, the obtained R-SiNPs could also achieve the detection of AA and KMnO 4 , and the LOD were 0.3 μM and 16 nM for AA and KMnO 4 , respectively.…”
Section: Results and Discussionmentioning
confidence: 99%
“…A significant linear correlation ( R 2 = 0.989) existed between the [log ( F – F 0 )] and the ACP concentration in the range of 1.0–50 mU/L. The limit of detection (LOD) was estimated to be 0.3 mU/L, which was lower than some fluorescence methods in Table . In addition, Figure illustrated that the established method displayed ascendant selectivity for ACP sensing. Interestingly, the obtained R-SiNPs could also achieve the detection of AA and KMnO 4 , and the LOD were 0.3 μM and 16 nM for AA and KMnO 4 , respectively.…”
Section: Results and Discussionmentioning
confidence: 99%