Sensitive and specific identification by polymerase chain reaction ofEimeria tenellaandEimeria maxima, important protozoan pathogens in laboratory avian facilities

Abstract

Eimeria tenella and Eimeria maxima are important pathogens causing intracellular protozoa infections in laboratory avian animals and are known to affect experimental results obtained from contaminated animals. This study aimed to find a fast, sensitive, and efficient protocol for the molecular identification of E. tenella and E. maxima in experimental samples using chickens as laboratory avian animals. DNA was extracted from fecal samples collected from chickens and polymerase chain reaction (PCR) analysis was employed to detect E. tenella and E. maxima from the extracted DNA. The target nucleic acid fragments were specifically amplified by PCR. Feces secreting E. tenella and E. maxima were detected by a positive PCR reaction. In this study, we were able to successfully detect E. tenella and E. maxima using the molecular diagnostic method of PCR. As such, we recommended PCR for monitoring E. tenella and E. maxima in laboratory avian facilities.

Prevalence of Eimeria species among sheep and goats in Suez Governorate, Egypt

“…Morphological detection and identification by flotation technique using saturated saline was effective for Eimeria detection despite the need for competent experience, time and effort [13] . This study aimed to detect the molecular amplification of the Eimeria using the ITS-1 rDNA had proven efficient results as well as it reduced time and effort [28] .…”

“…Recently, modern molecular techniques have been deployed for Eimeria identification including PCR amplification technique [12] . Nevertheless, the main issue with the PCR procedures is that the Eimeria oocyst wall is extremely robust in addition to the low DNA concentration obtained especially if the oocysts numbers are low in subclinical cases, thus a successful DNA extraction from oocysts is imperative for reliable PCR amplification and detection of Eimeria [13] .…”

The effect of infection with mixedEimeriaspecies on hematology and immune responses following Newcastle disease and infectious bursal disease booster vaccination in broilers

“…Coccidia of the genus Eimeria are obligatory protozoan parasites that parasitize the intestinal epithelium and are host-specific (Lee et al 2011). Avian coccidiosis, one of the most important parasitic diseases in chickens, is caused by seven different species of Eimeria.…”

Anticoccidial effects of the root bark ofDictamnus dasycarpusTurcz extract on experimentalEimeria tenellainfection

“…Fecal materials were collected from 6 to 10 days postinfection. The fecal samples were analyzed for the presence of coccidial oocysts using a standard fecal flotation technique [ 16 ]. Briefly, 5 mL from each sample was pelleted by centrifugation at 1500×g for 5 min.…”