Anticoccidial effects of the root bark of<i>Dictamnus dasycarpus</i>Turcz extract on experimental<i>Eimeria tenella</i>infection

Hong, Sunhwa; Lee, Hyun-A; Kim, Dongwoo; Oh, Gi-Wook; Kim, Okjin

doi:10.5625/lar.2014.30.4.169

Cited by 5 publications

(3 citation statements)

References 20 publications

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“…As an alternative to antibiotics, anticoccidial effect of various herbal extracts has been reported [31,[34][35][36][37][38][39] . Their effects were related to protection and/or relieve of intestinal mucosa.…”

Section: Feed Conversion Ratio (Fcr Feed: Bw Gain)mentioning

confidence: 99%

Usnik Asitin Broylerlerdeki Anticoccidial Etkinliği

Güven¹,

Avcıoğlu²,

Aslan³

et al. 2016

Kafkas Univ Vet Fak Derg

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This experiment was conducted to investigate if usnic acid, a lichen metabolite, exerts therapeutic action against coccidiosis. A total of 160 one-day-old male Ross 308 broiler chicks were divided into 5 experimental groups (A-E), each replicated in 4 pens of 8 chicks. At 16 days of age Groups B-E were infected orally with a mixture of purified oocysts including 30 x 10 4 sporulated oocysts from field isolates of E. acervulina, E. brunetti, E. maxima, E. mitis, E. praecox and E. tenella, whereas Group A was remained uninfected. Seven days after the coccidiosis induction, infected birds were divided into 4 groups to receive orally with 20 mg usnic acid (Group C), 100 mg usnic acid (Group D) and 7 mg toltrazuril (Group E) per kg body weight. The birds in Group B were untreated and served as infected control. The experiment was terminated at 29 days of age. As a result of the study it was evaluated that infected birds had lower feed intake and body weight and worse feed conversion, higher intestinal lesion score, longer small intestine and cecum, and higher fecal oocyst count than healthy birds. The anticoccidial effect of usnic acid at 100 mg/kg application dose was comparable to toltrazuril as reflected by alleviations in performance and pathology findings. In conclusion, it is demonstrated that usnic acid possesses some anticoccidial effects, but not nearly as good as toltrazuril. Keywords: Lichen, Usnic acid, Toltrazuril, Broiler, Coccidiosis Usnik Asitin Broylerlerdeki Anticoccidial Etkinliği ÖzetBu çalışmada, bir liken metaboliti olan usnik asitin coccidiosise karşı terapötik etkinliğinin araştırılması amaçlanmıştır. Toplam 160 adet, 1 günlük yaştaki erkek Ross 308 civciv, her bir grup 4 tekerrürlü olacak şekilde 5 deneysel gruba (A-E) ayrılmıştır. Hayvanlar 16 günlük yaşa geldiğinde Grup B-E'dekiler E. acervulina, E. brunetti, E. maxima, E. mitis, E. praecox ve E. tenella'nın saha izolatlarından elde edilmiş inokulumdan 30 x 10 4 sporlanmış oocyst ile enfekte edilmiştir. Grup A'da yer alanlar civcivler ise enfekte edilmeden kontrol olarak bırakılmıştır. Hastalık oluşturulduktan 7 gün sonra, enfekte hayvanlara ağız yolu ile 20 mg/kg usnik asit (Grup C), 100 mg/kg usnik asit (Grup D) ve 7 mg/kg toltrazuril (Grup E) uygulanmış; B grubundaki enfekte hayvanlara tedavi uygulanmadan enfekte kontrol olarak bırakılmıştır. Broylerler 29 günlük olduğunda çalışma sona erdirilmiştir. Enfekte hayvanların sağlıklı hayvanlara kıyasla daha düşük yem tüketimi, vücut ağırlığı ve yemden yararlanma oranına; daha yüksek lezyon skoruna, daha uzun bağırsak uzunluğuna ve daha yüksek oocyst sayısına sahip olduğu tespit edilmiştir. Performans ve patolojik bulgulardaki değişimler göz önünde bulundurulduğunda 100 mg/kg dozda uygulanan usnik asitin toltrazuril ile karşılaştırılabilecek derecede anticoccidal etkinliğe sahip olduğu belirlenmiştir. Sonuç olarak; usnik asitin bazı anticoccidial etkinliklere sahip olduğu ancak bu etkilerin toltrazuril kadar güçlü olmadığı kanısına varılmıştır.

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Section: Feed Conversion Ratio (Fcr Feed: Bw Gain)mentioning

confidence: 99%

Usnik Asitin Broylerlerdeki Anticoccidial Etkinliği

Güven¹,

Avcıoğlu²,

Aslan³

et al. 2016

Kafkas Univ Vet Fak Derg

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“…For several decade, owing to the better safety profile, medicinal plant‐derived natural products have excellent sources of therapeutic drugs for various diseases including cancer 18,19 . Root bark of mulberry ( Morus alba L.) has been employed as natural product medicine with various therapeutic activities such as antioxidation, 20 anti‐inflammation, 21 neuroprotection, 22 immunomodulation, 23 and antiatherosclerosis, 24,25 antiobesity, 25 antitumor, 26 antidepressant, 27,28 antihyperglycemic, and hypolipidemic activities 29 . Among various constituents of Morus alba , including Kuwanon derivatives such as Kuwanon‐C, and G have shown anticancer activity 26 .…”

Section: Introductionmentioning

confidence: 99%

Morus alba derived Kuwanon‐A combined with 5‐fluorouracil reduce tumor progression via synergistic activation of GADD153 in gastric cancer

Thakur²,

Pan

et al. 2023

MedComm – Oncology

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Despite the application of conventional strategies including chemotherapy, radiotherapy, surgery, or immunotherapy, the mortality of gastric cancer (GC) patients remains high. Often, GC is not diagnosed until it has reached late stage, resulting in a missed surgical window. Therefore, a new therapeutic intervention for GC is necessary. Here, the combined application of Kuwanon‐A (KA) and 5‐fluorouracil (5‐FU) was evaluated for its potential to combat GC for the first time. To determine the anticancer activity of KA (from Morus alba) along with 5‐FU against GC, and their mechanism via GADD153, we examained anticancer potential of KA along with 5‐FU via in vitro assays with GC cells, namely MKN‐45, SGC‐7901, HGC‐27, and BGC‐823, and in vivo assays with mouse xenograft of GC. KA alone could induce G2/M phase arrest and apoptosis in GC cells by activating GADD153 through the PERK/elF2α/ATF4 and IRE1/XBP1 signaling pathways, suggesting a critical role of increased endoplasmic reticulum stress in KA‐induced apoptosis of GC cells. Moreover, the combination of KA and 5‐FU showed an enhanced synergistic anticancer effect against GC both in vitro and in vivo. Conclusively, the combination of KA and 5‐FU can act as an effective anticancer regimen in combating GC.

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“…[ 13 ] The known constituents of D. dasycarpus root bark include limonoids,[ 7 10 14 15 16 17 ] furoquinoline alkaloids,[ 17 ] flavonoids,[ 15 16 ] coumarins,[ 18 ] sesquiterpenes,[ 19 ] sesquiterpene glycosides, and phenolic glycosides. [ 19 20 21 ]…”

Section: Introductionmentioning

confidence: 99%

Apoptosis of AGS human gastric adenocarcinoma cells by methanolic extract of Dictamnus

Park¹,

Hong²,

Ahn³

et al. 2015

Phcog Mag

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Background:The root bark of Dictamnus dasycarpus Turcz has traditionally been used in East Asia to treat skin diseases such as eczema, atopic dermatitis, and psoriasis. However, it has also been reported to exhibit an anti-proliferative effect on cancer cells.Objective:To investigate the anti-cancer effects of a methanol extract of Dictamnus dasycarpus root bark (MEDD) on AGS cells (a human gastric adenocarcinoma cell-line).Materials and Methods:An 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium assay, a caspase activity assay, cell cycle analysis, mitochondrial membrane potential (MMP) measurements, and western blotting were used to investigate the anti-cancer effects of MEDD on AGS cells.Results:Treatment with MEDD significantly and concentration-dependently inhibited AGS cell growth. MEDD treatment in AGS cells led to increased accumulation of apoptotic sub-G1 phase cells in a concentration-dependent manner. Also, MEDD reduced the expressions of pro-caspase-3, -8 and -9, and increased the active form of caspase-3. Furthermore, subsequent Western blotting revealed elevated levels of poly (ADP-ribose) polymerase protein. MEDD treatment reduced levels of MMP and anti-apoptotic Bcl-2 and Bcl-xL proteins. Pretreatment with SB203580 (a specific inhibitor of p38 mitogen-activated protein kinases), SP600125 (a potent inhibitor of C-Jun N-terminal kinases), or PD98059 (a potent inhibitor of extracellular signal-regulated kinases) did not modify the effects of MEDD treatment. However, pretreatment with LY294002 (a specific inhibitor of Akt) significantly enhanced MEDD-induced cell death.Conclusion:These results suggest that MEDD-mediated cell death is associated with the intrinsic apoptotic pathway and that inhibition of Akt signaling contributes to apoptosis induction by MEDD.

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Anticoccidial effects of the root bark ofDictamnus dasycarpusTurcz extract on experimentalEimeria tenellainfection

Cited by 5 publications

References 20 publications

Usnik Asitin Broylerlerdeki Anticoccidial Etkinliği

Usnik Asitin Broylerlerdeki Anticoccidial Etkinliği

Morus alba derived Kuwanon‐A combined with 5‐fluorouracil reduce tumor progression via synergistic activation of GADD153 in gastric cancer

Apoptosis of AGS human gastric adenocarcinoma cells by methanolic extract of Dictamnus

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