Sensitive and Simple Detection of Glucose Based on Single Plasmonic Nanorod

Abstract

A simple and sensitive method to determinate glucose content was developed based on single plasmonic nanoparticles by conventional dark-field microscopy (DFM). An enzyme-responsive plasmonic Ag/Au bilayer of rods was designed and prepared. Their localized surface plasmon resonance (LSPR) could be tailored by the enzyme reaction of glucose oxidase (GOx) by finely tuning the morphology and plasmonic optical response of the hybrid nanostructure. It was found that the plasmon resonance scattering (PRS) spectra peak (λmax) shifted to longer wavelengths under enzyme reactions, and the degree of the shift were proportional to the content of glucose. This approach is convenient to study the local concentration of glucose in real time. Keywords

Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current Updates

“…Recently, the real-time monitoring of glucose concentrations in GOD based biosensors has been achieved through the use of plasmonic nanoparticles/nanorods (Xu et al, 2017b). A list of major nano-materials, CNTs, and carbon nano-fibers (CNFs) used as electrical connectors between the electrode and the redox center, are given in Table 3.…”

Optical Sensing Platform for the Colorimetric Determination of Silver Nanoprisms and Its Application for Hydrogen Peroxide and Glucose Detections Using a Mobile Device Camera

“…The unique optical property of metal nanoparticles, called localized surface plasmon resonance (LSPR), is based on the coupling between an external electromagnetic field and a collective electron oscillation in the conduction band of metal nanoparticles. [1][2][3][4][5][6][7][8][9][10] The resulting local electric field is very sensitive in terms of a dielectric function, i.e., any eventual environment alteration leads to detectable changes in their optical properties. 1,2,[6][7][8] Colloidal solutions of silver and gold nanoparticles, for instance, have unique optical characteristics in the visible light region that enables their LSPR properties alteration to be monitored using UV-visible spectrometry and cameras.…”

“…1,2,[6][7][8] Colloidal solutions of silver and gold nanoparticles, for instance, have unique optical characteristics in the visible light region that enables their LSPR properties alteration to be monitored using UV-visible spectrometry and cameras. [10][11][12][13][14][15][16][17] Colorimetric detection, benefiting from LSPR phenomenon of plasmonic nanomaterials, are being utilized for biosensor applications. [10][11][12][13][14][15][16][17][18][19][20][21] For example, Sittiwong and Unob utilized a colorimetric approach and spectroscopic analysis for the determination of urinary creatinine.…”

“…[10][11][12][13][14][15][16][17] Colorimetric detection, benefiting from LSPR phenomenon of plasmonic nanomaterials, are being utilized for biosensor applications. [10][11][12][13][14][15][16][17][18][19][20][21] For example, Sittiwong and Unob utilized a colorimetric approach and spectroscopic analysis for the determination of urinary creatinine. 12 The creatinine in human urine was extracted by solid-phase extraction for the elimination of interfering effects from the urine matrix on creatinine detection.…”

“…19,37 Moreover, for some biomolecules, such as glucose and cholesterol, which are the major chemicals to act as the indicators for medical diagnosis, H2O2 is one of products from the enzymatic reaction when they are oxidized by their oxidase enzymes. 10,19,38,39 Since the calculated IA value was sensitive to the concentration of AgNPrs, employing H2O2 etching and correspondent oxidase enzyme reactions, we used this value to quantitatively determine the H2O2 and glucose concentrations. The camera-based detection system developed, based on the optical characteristic of AgNPrs, showed to be a simple, rapid, and reliable method, revealing great potential for biochemical analysis, clinical diagnosis, and household biosensor applications.…”