Sensitive and Selective Reversed-Phase High Performance Liquid Chromatographic-UV Spectrophotometric Determination of Dextromethorphan and its CYP2D6 Mediated Metabolite, Dextrorphan in Human Urine

Ebeshi, Benjamin U.; Obodozie, Obiageri O.; Bolaji, Oluseye O.

doi:10.4314/tjpr.v13i2.18

Cited by 4 publications

(1 citation statement)

References 15 publications

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“…A sensitive LC-MS/MS method has been reported to assay DXM and three metabolites (DXO, 3-methoxymorphinan and 3-hydroxymorphinan) in human urine [38]. DXM and its metabolite, DXO were determined in human urine also by using a sensitive and selective reversed-phase high performance liquid chromatography with UV-spectrophotometric detection [10]. A gas chromatography-mass spectrometry confirmation method for the detection of DXM and DXO in urine and oral fluid was reported [28].…”

Section: Introductionmentioning

confidence: 99%

Screening Method for the Detection of Dextromethorphan Abuse by HPTLC

STAN¹

2022

FARMACIA

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Dextromethorphan (DXM), the methylated dextrorotatory analogue of a μ-opioid receptor agonist, levorphanol, is a widely available antitussive medicine. At higher levels compared to therapeutic doses, DXM acts as a dissociative hallucinogen and has been shown to be often abused by individuals seeking this particular type of effects. Some reports indicate that DXM causes false positives results in the initial immunoassay urine screening for drugs of abuse. The paper presents a new high performance thin layer chromatographic method (HPTLC) for the determination of DXM in urine samples collected from DXM abusers. The separation has been achieved on chromatographic glass plates precoated with silicagel 60 F254 using as solvent system the mixture methanol: ammonia (100:1.5, v/v). The detection was performed by densitometry in the absorbance mode at 278 nm. An unknown spot, presumably a metabolite of DXM was detected in all urine samples, suggesting that the subjects could be extensive DXM metabolizers. The results indicate that the proposed HPTLC method is a simple and rapid procedure that can be applied as a screening tool for an analytical diagnostic in DXM abuse or intoxication. RezumatDextrometorfanul (DXM), analogul dextrogir metilat al levorfanolului, unui agonist al receptorului μ-opioid, este un medicament antitusiv disponibil pe scară largă. La doze mai mari față de dozele terapeutice, DXM acționează ca un halucinogen disociativ și s-a dovedit că este adesea cunsumat abuziv de către persoanele care caută acest tip special de efecte. Unele rapoarte indică faptul că screening-ul inițial al drogurilor de abuz în urină prin imunoanalize poate conduce rezultate fals pozitive în cazul DXM. Lucrarea de față prezintă o nouă metodă cromatografică în strat subțire de înaltă performanță (HPTLC) pentru determinarea DXM în probele de urină colectate de la subiecți care au consumat DXM în mod abuziv. Separarea a fost realizată pe plăci cromatografice de sticlă preacoperite cu silicagel 60 F254, utilizând ca sistem de solvenți amestecul metanol:amoniac (100:1,5, v/v). Detecția s-a făcut densitometric, în modul de absorbanță, la 278 nm. Un spot neidentificat, datorat probabil unui metabolit al DXM, a fost detectat în toate probele de urină analizate, sugerând că subiecții ar putea fi metabolizatori extensivi ai DXM. Rezultatele indică faptul că metoda HPTLC propusă este un procedeu simplu și rapid care poate fi aplicat ca instrument de screening pentru un diagnostic analitic în abuzul sau intoxicația cu DXM.

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Section: Introductionmentioning

confidence: 99%

Screening Method for the Detection of Dextromethorphan Abuse by HPTLC

STAN¹

2022

FARMACIA

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Low‐volume plasma sampling for determination of dextromethorphan and dextrorphan in rat plasma: LC–MS/MS method and its application in pharmacokinetic study

Majhi

Sayyad²,

Mishra³

et al. 2023

Biomedical Chromatography

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Dextromethorphan (DM) and its metabolite dextrorphan (DX) continue to draw the attention of researchers owing to their diverse pharmacodynamics. Thus, there are possibilities for repurposing DM. Most of the pharmacodynamics of DM needs further validation in different preclinical models. Also, it is necessary to correlate the pharmacodynamics with relevant pharmacokinetics data. Multiple bioanalytical techniques developed for this purpose primarily use a high sample processing volume. Since sample volume is a limiting factor for many preclinical models, an effort was taken to develop an alternative method suitable for handling low sample processing volumes. An efficient solid‐phase extraction technique, robust liquid chromatographic (LC) separation and highly sensitive tandem mass spectrometric detection (MS/MS) showed suitability for use of a 30 μl sample processing volume. This led to the development of a highly specific, selective, accurate and precise‐bio‐analytical method for simultaneous quantification of DM and DX in rat plasma. The validated method was linear in the range of 0.196–403.356 ng/ml for DM and 0.102–209.017 ng/ml for DX. The application of the method was demonstrated through the estimation of pharmacokinetic parameters that showed good congruence with earlier studies.

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Development and validation of stability indicating HPTLC method for estimation of dextromethorphan hydrobromide

2017

JAPR

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Sensitive and Selective Reversed-Phase High Performance Liquid Chromatographic-UV Spectrophotometric Determination of Dextromethorphan and its CYP2D6 Mediated Metabolite, Dextrorphan in Human Urine

Abstract: Purpose: To develop a simple, sensitive and selective method for the determination of dextromethorphan and its metabolite, dextrophan in human urine using reversed-phase high performance liquid chromatography with UV-spectrophotometric detection (RP-HPLC-UV

Cited by 4 publications

References 15 publications

Screening Method for the Detection of Dextromethorphan Abuse by HPTLC

Screening Method for the Detection of Dextromethorphan Abuse by HPTLC

Low‐volume plasma sampling for determination of dextromethorphan and dextrorphan in rat plasma: LC–MS/MS method and its application in pharmacokinetic study

Development and validation of stability indicating HPTLC method for estimation of dextromethorphan hydrobromide

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