Sensitive and label-free chemiluminescence detection of malathion using exonuclease-assisted dual signal amplification and G-quadruplex/hemin DNAzyme

Insertion of Hemin into Metal–Organic Frameworks: Mimicking Natural Peroxidase Microenvironment for the Rapid Ultrasensitive Detection of Uranium

“…17,18 So far, to address this problem, various hemin-contained peroxidase mimetics have been developed. 15,19−21 But common approaches to the manufacture of these hemin-contained artificial enzymes relied on the use of biological macromolecules, such as DNA, 22,23 proteins, 24 and polypeptides, 25−27 still facing the vulnerability of biological macromolecules. Such insufficient stability has limited the wide range of applications of artificial enzymes in areas including biosensing, bioimaging, and environmental analysis.…”

“…The “bottom-up” approach for the fabrication of such a hemin-based peroxidase mimetic catalyst allowed researchers to not only mimic catalytic function but also the active site of natural peroxidases, offering a more in-depth understanding of the genesis of the natural enzyme. Unfortunately, the requirement for structurally and chemically reproducing the active sites of natural enzymes makes the mimetic process challenging. , So far, to address this problem, various hemin-contained peroxidase mimetics have been developed. ,− But common approaches to the manufacture of these hemin-contained artificial enzymes relied on the use of biological macromolecules, such as DNA, , proteins, and polypeptides, − as the backbone to mimic the microenvironment of the natural peroxidase active center, still facing the vulnerability of biological macromolecules. Such insufficient stability has limited the wide range of applications of artificial enzymes in areas including biosensing, bioimaging, and environmental analysis.…”

An aptamer and flower-shaped AuPtRh nanoenzyme-based colorimetric biosensor for the detection of profenofos

“…Wu et al constructed an aptamer-based chemiluminescence sensor based on exonuclease assisted double signal amplification. [14][15][16] An aptamer probe and hairpin probe successfully identified malathion and realized the signal amplification and detection. The detection sensitivity was high and the limit of detection was as low as 0.47 pM.…”

A label‐free and signal‐amplifiable assay method for colorimetric detection of carcinoembryonic antigen

“…These advantages make it possible for hemin/ G-quadruplexes to serve as a versatile toolkit in biosensing and biomedical applications. Therefore, hemin/G-quadruplexes have been widely utilized in fluorescence (Bai et al, 2021;Chen et al, 2020a,b), colorimetric (Zhu et al, 2020), electrochemical (Ma et al, 2020), and chemiluminescence (Wu et al, 2021;Zhang et al, 2021) biosensing systems for the detection of a wide range of targets, including small molecules, metal ions, tumor markers, DNA, exosomes, and others. It is noted that the combination of Gquadruplex with other amplification technologies provides a new idea for the design of biosensors with super ior performance.…”