Sensitive and fast identification of bacteria in blood samples by immunoaffinity mass spectrometry for quick BSI diagnosis

Zhu, Yingdi; Qiao, Liang; Prudent, Michel; Bondarenko, Alexandra; Gasilova, Natalia; Möller, Siham Beggah; Lion, Niels; Pick, Horst; Gong, Tianqi; Chen, Zhuoxin; Yang, Pengyuan; Lovey, Lysiane Tissières; Girault, Hubert H.

doi:10.1039/c5sc04919a

Cited by 59 publications

(45 citation statements)

References 29 publications

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“…IMS is suitable for enrichment of target bacterial cells and in combination with some progressive tools for bacteria identification. For example, matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) can significantly shorten blood stream infection diagnosis by skipping blood culture methods [ 192 ]. Due to extremely high affinity and low-cost, bacteriophages were used as a biorecognition element for bacteria [ 193 ].…”

Section: Magnetic Nanoparticles In the Real Worldmentioning

confidence: 99%

Magnetic Nanoparticles: From Design and Synthesis to Real World Applications

et al. 2017

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The increasing number of scientific publications focusing on magnetic materials indicates growing interest in the broader scientific community. Substantial progress was made in the synthesis of magnetic materials of desired size, morphology, chemical composition, and surface chemistry. Physical and chemical stability of magnetic materials is acquired by the coating. Moreover, surface layers of polymers, silica, biomolecules, etc. can be designed to obtain affinity to target molecules. The combination of the ability to respond to the external magnetic field and the rich possibilities of coatings makes magnetic materials universal tool for magnetic separations of small molecules, biomolecules and cells. In the biomedical field, magnetic particles and magnetic composites are utilized as the drug carriers, as contrast agents for magnetic resonance imaging (MRI), and in magnetic hyperthermia. However, the multifunctional magnetic particles enabling the diagnosis and therapy at the same time are emerging. The presented review article summarizes the findings regarding the design and synthesis of magnetic materials focused on biomedical applications. We highlight the utilization of magnetic materials in separation/preconcentration of various molecules and cells, and their use in diagnosis and therapy.

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Section: Magnetic Nanoparticles In the Real Worldmentioning

confidence: 99%

Magnetic Nanoparticles: From Design and Synthesis to Real World Applications

et al. 2017

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“…Custom-made reference spectra thus allow ample flexibility with respect to different sample preparation protocols and experimental set-ups in a particular lab or for specific applications. For example, reference spectra for different cell densities of a particular organism may allow an estimation of cell densities [20]. It is of course also possible to generate reference spectra for fungi grown on different agar plates, different physiological states, different extraction protocols, or directly applied bacterial or yeast cells (as opposed to crude extracts).…”

Section: Custom-made Reference Spectra Improve Maldi-tof Ms-based Idementioning

confidence: 99%

“…In other studies, 97 to 69.5% of bacteria were correctly identified after a short incubation of positive blood cultures on solid media of only 3 to 5 h [53][54][55][56], which enabled identification on the same day as the positive blood sample was detected. By increasing the sample concentration (by reducing the sample spot size), creating reference spectra for different cell densities, and immunoaffinity enrichment of bacteria, it was possible to detect as few as 10 to 100 bacterial cells after a blood culture time of only 4 h [20]. These examples illustrate the vastly shortened time-to-result of clinical MALDI-TOF MS applications: the duration of the cultivation step has been reduced to just a few hours and is thus in the same range as DNA amplification steps.…”

Section: Shortening the Cultivation Time For Faster Maldi-tof Ms Idenmentioning

confidence: 99%

“…In order to increase the number of mass peaks that may be used as biomarkers or the sensitivity of a MALDI-TOF MS analysis, samples have been successfully treated with detergent, sonication, corona plasma discharge, or heat [78][79][80]. In another study, target bacteria were separated and enriched without prior cultivation by using magnetic nanobeads that were functionalized with specific antibodies [20]. A rapid sample pretreatment consisting of removal of interfering blood cells and centrifugation and washing steps, which can also be performed with a commercially available kit (Sepsityper), is crucial for obtaining high identification rates of microorganisms, including yeasts, directly in positive blood cultures [81,82].…”

Section: Sample Processing Can Broaden the Use Of Maldi Biotypingmentioning

confidence: 99%

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MALDI-TOF mass spectroscopy of yeasts and filamentous fungi for research and diagnostics in the agricultural value chain

Drissner

Freimoser

2017

Chem. Biol. Technol. Agric.

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Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS; MALDI biotyping) has become a standard tool for the accurate, rapid, and economical identification of pathogens in the clinical diagnostics laboratory. The method is continuously being improved, and new applications for distinguishing strains, identifying metabolites or functional characteristics (e.g., antibiotic resistance), and detecting microbes directly in patient samples have been developed. Adopting these methods in other disciplines than clinical diagnostics, for example, in agriculture, food safety and quality testing, or ecology, will open up new opportunities for diagnostics and research. This review focuses on MALDI-TOF MS approaches for the identification of yeasts and filamentous fungi. In contrast to bacterial diagnostics, MALDI biotyping of fungi is more challenging and less established. We thus start by discussing the role of MALDI-TOF MS as a tool for species identification; in particular with respect to DNA-based identification methods. The review then highlights the value of custom-made reference spectra for MALDI biotyping and points out recent advancements of MALDI-TOF MS, mainly from the field of clinical diagnostics that may be adopted and used for fungal diagnostic challenges. The overview ends with a summary of MALDI-TOF MS studies of yeasts and filamentous fungi of agricultural relevance.

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“…Colony morphology (form, elevation, and appearance), gram stain, and biochemical phenotyping are culture-based techniques to classify and identify the bacteria. Molecular approaches, such as multiplex PCR and mass spectroscopy, can be performed with isolated bacteria to identify strains (13)(14)(15)(16)(17). To determine the antimicrobial resistance of the pathogen, the growth of the pathogen in the presence of antibiotics is interpreted and reported for therapeutic management of the patient (18)(19)(20)(21).…”

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confidence: 99%

Adaptable microfluidic system for single-cell pathogen classification and antimicrobial susceptibility testing

Torab

Mach

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

104

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Infectious diseases caused by bacterial pathogens remain one of the most common causes of morbidity and mortality worldwide. Rapid microbiological analysis is required for prompt treatment of bacterial infections and to facilitate antibiotic stewardship. This study reports an adaptable microfluidic system for rapid pathogen classification and antimicrobial susceptibility testing (AST) at the single-cell level. By incorporating tunable microfluidic valves along with real-time optical detection, bacteria can be trapped and classified according to their physical shape and size for pathogen classification. By monitoring their growth in the presence of antibiotics at the single-cell level, antimicrobial susceptibility of the bacteria can be determined in as little as 30 minutes compared with days required for standard procedures. The microfluidic system is able to detect bacterial pathogens in urine, blood cultures, and whole blood and can analyze polymicrobial samples. We pilot a study of 25 clinical urine samples to demonstrate the clinical applicability of the microfluidic system. The platform demonstrated a sensitivity of 100% and specificity of 83.33% for pathogen classification and achieved 100% concordance for AST.

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Sensitive and fast identification of bacteria in blood samples by immunoaffinity mass spectrometry for quick BSI diagnosis

Abstract: An immunoaffinity mass spectrometry method was proposed for quick BSI diagnosis.

Cited by 59 publications

References 29 publications

Magnetic Nanoparticles: From Design and Synthesis to Real World Applications

Magnetic Nanoparticles: From Design and Synthesis to Real World Applications

MALDI-TOF mass spectroscopy of yeasts and filamentous fungi for research and diagnostics in the agricultural value chain

Adaptable microfluidic system for single-cell pathogen classification and antimicrobial susceptibility testing

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