Sensitive and direct determination of lithium by mixed-mode chromatography and charged aerosol detection

Abstract

A sensitive analytical method using mixed mode HPLC separation coupled with charged aerosol detection (CAD) was developed for quantitative analysis of lithium. The method is capable of separating lithium ion from different drug matrices and other ions in a single run thus eliminating the organic matrix and ionic analyte interferences without extensive sample preparation such as derivatization and extraction. The separation space and chromatographic conditions are defined by systematic studies of the retention behaviors of lithium and potential interfering ions and different type of pharmaceutical APIs (active pharmaceutical ingredients) under reversed-phase, HILIC and cation/anion exchange mechanisms. Compared to other current analytical techniques for lithium analysis, the presented method provides a new approach and demonstrates high sensitivity (0.02ng for LOD and 0.08ng for LOQ in both standard and sample solution). The method has been validated for pharmaceutical samples and can be potentially applied to biological, food and environmental samples.

Development and validation of a stability‐indicating HPLC method for topiramate using a mixed‐mode column and charged aerosol detector

“…The main goal of this study was to develop alternative stability‐indicating HPLC methods for topiramate and its DPs for detection by CAD that would reduce the total number of methods (four HPLC methods) and the total number of detectors (two detectors, refractive index (RI), and conductivity) required by USP for the analysis of the topiramate raw material. The ATP1 column was chosen for this study due to its superior performance in comparison with other multimodal chromatographic columns and its successful application to the simultaneous separation of drugs and counterions with a broad range of hydrophobicity . Several chromatographic conditions were evaluated for topiramate and its DP (Table ).…”

Aplicaciones De La Química Supramolecular: Síntesis De Un Sensor Con Propiedades Fluorescentes en Solución Y en Fase Sólida Para La Detección Selectiva De Litio

“…La evaluación de la respuesta sensora del ligante L1 con los metales Li + , Na + y K + se llevó a cabo un estudio cualitativo de la formación del complejo. Se estudiaron adicionales a Li + los cationes Na + y K + , porque en la recuperación del Li + se utilizan Na 2 CO 3 y K 2 CO 3 , para formar Li 2 CO 3 , por lo que es importante conocer si el ligante presenta respuesta por la presencia de estos iones [10] [11]. La figura 5 muestra los espectros de emisión del ligante L1 libre y de los complejos formados en solución.…”

Assessment of Lithium, Macro- and Microelements in Water, Soil and Plant Samples from Karst Areas in Romania

“…Li concentrations can be determined using different analytical techniques such as atomic absorption spectrometry (AAS), inductively coupled plasma atomic emission spectrometry (ICP-AES), and inductively coupled plasma mass spectrometry (ICP-MS) or by ion-selective electrodes [ 15 ]. Of these, the ICP-MS technique is a sensitive method that was frequently used for determination of elements from various types of samples, such as water, wine, saliva, blood, urine, plant, sediment, and rocks [ 2 ].…”