Sensing Specific Adhesion of Liposomal and Micellar Systems with Attached Carbohydrate Recognition Structures at Lectin Surfaces

Hildebrand, Annegret; Schaedlich, Anita; Rothe, Ulrich; Neubert, Reinhard H.H.

doi:10.1006/jcis.2002.8272

Cited by 22 publications

(20 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Free CaY molecules were present in TBS buffer injected into a QCM liquid cell setup. Two methods of protein immobilization on a gold surface of the quartz crystal electrode have been chosen: (i) the direct adsorption of Con A on gold [35] and (ii) the covalent binding of Con A using thiol groups and glutaraldehyde as cross-linking agents [32]. The quality of the protein immobilization for these methods has been verified using both QCM and AFM.…”

Section: Protein Depositionmentioning

confidence: 99%

Lectin–carbohydrate affinity measured using a quartz crystal microbalance

Lebed¹,

Kulik²,

Forró³

et al. 2006

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

Section: Protein Depositionmentioning

confidence: 99%

Lectin–carbohydrate affinity measured using a quartz crystal microbalance

Lebed¹,

Kulik²,

Forró³

et al. 2006

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

“…The technique offers labelfree binding measurements in real-time, with high sensitivity and reliability. Carbohydrate recognition has only relatively been targeted recently with QCM methods (Bakowsky et al, 2000;Hashizume et al, 1998;Hildebrand et al, 2002;Miura et al, 2002;Steinem et al, 1997) showing that these systems are well suited for such analyses.…”

Section: Introductionmentioning

confidence: 99%

Study of real-time lectin–carbohydrate interactions on the surface of a quartz crystal microbalance

Pei

Anderson

Aastrup

et al. 2005

Biosensors and Bioelectronics

View full text Add to dashboard Cite

“…[15][16][17][18][19][20][21][22][23] Among these methods, the quartz crystal microbalance (QCM) biosensor format is being increasingly adopted due to its convenient means of operation coupled with high performance. [24][25][26][27][28][29][30][31][32][33] Measurements can also be performed in real time using native analytes without the need of any labeling procedures.Efficient carbohydrate immobilization techniques to the sensor surface are a prerequisite for the preparation of biosensor systems. In principle, three general strategies can be used to enable carbohydrate presentation at sensor surfaces: direct covalent conjugation of carbohydrate structures to the gold electrode, noncovalent adsorption of carbohydrates on a modified surface, and covalent conjugation of carbohydrate-derivatives to a modified surface.…”

mentioning

confidence: 99%

Photoderivatized Polymer Thin Films at Quartz Crystal Microbalance Surfaces: Sensors for Carbohydrate−Protein Interactions

Pei

et al. 2007

Anal. Chem.

View full text Add to dashboard Cite

Photoderivatized polymer-coated gold surfaces have been developed following a perfluorophenylazide-based double ligation strategy. Gold-plated quartz crystal microbalance (QCM) crystals were initially covalently functionalized with a monolayer of poly(ethylene glycol) (PEG), using photo-or thermolytic nitrene formation and insertion. The polymer surfaces were subsequently used as substrates for photoinsertion of carbohydrate-derivatized photoprobes, yielding different recognition motifs for selective protein binding. The resulting robust and biocompatible sensor surfaces were applied to a flow-through QCM instrument for monitoring lectin-carbohydrate interactions in real time. The results clearly show the predicted lectin selectivity, demonstrating the applicability of the approach.Selective surface generation and functionalization form the basis for intense research in a number of diverse areas such as chemical sensors, interface engineering, and nanotechnology. A versatile method to covalent derivatization is based on the specific chemistry of arylazides. Upon thermal activation or light irradiation, the azide functionality in these structures becomes converted to a highly reactive nitrene species that readily inserts into CH and NH bonds. 1 Especially perfluorophenylazides (PFPAs), 2 which produce markedly enhanced insertion yields, have thus been used to thermo-and photochemically introduce functional groups in a range of entities, including proteins, nanostructures, and synthetic polymers. [3][4][5][6][7][8][9] The versatility of the PFPA chemistry makes it an attractive choice for surface modification. Surfaces can either be globally modified by the technique, or discrete areas can be addressed by arraying techniques such as photomasking and conventional printing devices. HHS Public Access Author Manuscript Author ManuscriptAuthor Manuscript Author ManuscriptThe surface presentation of recognition motifs based on bioactive compounds is central for many applications in bioanalytical and biomaterials science, and carbohydrates and glycomimetic structures are in this context attracting increasing interest. [10][11][12][13][14] Interactions between cellular glycans and proteins have been found to be of special importance in many biological processes, being involved in cell-cell interactions, cell communication, cell proliferation, and cell death. This emerging significance prompts for new means to analytically study these interactions. To date, various methods have been developed to analyze interactions of carbohydrates and proteins, including biosensors, enzyme-linked lectin assays (ELLAs) and cell assays, nuclear magnetic resonance, calorimetric techniques, and, more recently, microarray technologies. [15][16][17][18][19][20][21][22][23] Among these methods, the quartz crystal microbalance (QCM) biosensor format is being increasingly adopted due to its convenient means of operation coupled with high performance. [24][25][26][27][28][29][30][31][32][33] Measurements can also be performed in real tim...

show abstract

Sensing Specific Adhesion of Liposomal and Micellar Systems with Attached Carbohydrate Recognition Structures at Lectin Surfaces

Cited by 22 publications

References 40 publications

Lectin–carbohydrate affinity measured using a quartz crystal microbalance

Lectin–carbohydrate affinity measured using a quartz crystal microbalance

Study of real-time lectin–carbohydrate interactions on the surface of a quartz crystal microbalance

Photoderivatized Polymer Thin Films at Quartz Crystal Microbalance Surfaces: Sensors for Carbohydrate−Protein Interactions

Contact Info

Product

Resources

About