Semiquantitative GATA-3 Immunoreactivity in Breast, Bladder, Gynecologic Tract, and Other Cytokeratin 7–Positive Carcinomas

Clark, Beth Z.; Beriwal, S.; Dabbs, David J.; Bhargava, Rohit

doi:10.1309/ajcp8h2vbdsciobf

Cited by 87 publications

(75 citation statements)

References 19 publications

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“…At the same time, ER-α is engaged in a positive cross-regulatory loop with GATA-3 as it also directly stimulates the transcription factor GATA-3, indicating that GATA-3 in association with ER can regulate critical genes in hormone-responsive breast cancer with statistically significant correlation between GATA-3 and ER expression [4,7,13,14]. GATA-3 has been developed as an immunohistochemical marker for breast and urothelial carcinomas for use in routine diagnostic pathology practice [8,15]. Although breast cancer is common, MAM and GCDFP-15 lack the desired sensitivity while ER and PR serve as prognostic markers with only adjunctive diagnostic utility, especially in the setting of a high-grade triple negative metastatic breast cancer.…”

Section: Discussionmentioning

confidence: 99%

“…Our study found that GATA-3 sensitivity in ER negative breast cancer cases to be 62.5 % compared to 42.8 % and 50 % for MAM and GCDFP-15 respectively; however, this needs to be interpreted cautiously due to the small number of ER negative cases in our cohort. The challenge with GATA-3 is its lack of tissue specificity as it can be expressed in urothelial carcinomas, pulmonary carcinomas, mesotheliomas, salivary gland tumors, and all skin adnexal tumors [5,6,8,15,17]. In the right morphologic and clinical setting GATA-3 has proven to be a useful marker in distinguishing breast cancer from other non-mammary carcinomas.…”

Section: Discussionmentioning

confidence: 99%

“…GATA-3 has recently been identified as a multi-specific but successful immunohistochemical marker for breast and urothelial differentiation [5,6]. It is also expressed in a subset squamous cell carcinomas, salivary gland tumors, mesotheliomas, gynecologic carcinomas and skin adnexal tumors [5,7,8]. In spite of its multi-specific nature, GATA-3 was recently reported to have high positive predicitve value (96.2 %) in determining the breast origin in serous malignant effusion [9].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of GATA-3, mammaglobin, GCDFP-15 expression in breast carcinoma in serous effusions: A cell-block micro-array study

Hag

et al. 2017

Pleura and Peritoneum

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Background: GATA-3 is a potential marker for detection of metastatic breast carcinoma, reportedly more sensitive than mammaglobin (MAM) and GCDFP-15. We aim to compare the sensitivity of GATA-3, MAM and GCDFP-15 in determining the breast origin of malignant effusions. Methods: Cell blocks from 27 cases of serous effusions positive for metastatic breast cancer were retrieved. Immunohistochemistry for GATA-3, MAM, gross cystic disease fluid protein 15 (GCDFP-15), estrogen receptor (ER) and progesterone receptor (PR) was performed on cell-block micro-array. Statistical analysis using two ways Chi square, one-way ANOVA and multiple regression was performed. Results: The detection rate of breast cancer in serous fluid was significantly higher with GATA-3 (88.8 %, X2 = 15.9, p = 0.00034) than with MAM (51.8 %) and GCDFP-15 (37.0 %). All ER positive cases (19) were GATA-3 positive. Conversely, all GATA-3 negative cases (3) were ER negative. The intensity of stain and percentage of positive cells were significantly higher with GATA-3 (p < 0.0001) than with MAM and GCDFP-15. The intensity and percentage of positive cells score of GATA-3 were statistically associated with ER stain intensity and percentage of positive cell scores.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of GATA-3, mammaglobin, GCDFP-15 expression in breast carcinoma in serous effusions: A cell-block micro-array study

Hag

et al. 2017

Pleura and Peritoneum

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“…Care must be taken in evaluating the teratoma data, however. Many human tumors express CGB (53)(54)(55), and some secrete placental CG and CSH1 (56), whereas others, including aggressive breast carcinomas, contain areas that are both GATA3 + and KRT7 + (57). Because the areas of teratomas expressing trophoblast markers were sporadic and not well characterized histologically, the proof that they represent fetal placenta remains weak.…”

Section: Discussionmentioning

confidence: 99%

Heightened potency of human pluripotent stem cell lines created by transient BMP4 exposure

Yang

Adachi

Sheridan

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Human pluripotent stem cells (PSCs) show epiblast-type pluripotency that is maintained with ACTIVIN/FGF2 signaling. Here, we report the acquisition of a unique stem cell phenotype by both human ES cells (hESCs) and induced pluripotent stem cells (iPSCs) in response to transient (24-36 h) exposure to bone morphogenetic protein 4 (BMP4) plus inhibitors of ACTIVIN signaling (A83-01) and FGF2 (PD173074), followed by trypsin dissociation and recovery of colonies capable of growing on a gelatin substratum in standard medium for human PSCs at low but not high FGF2 concentrations. The self-renewing cell lines stain weakly for CDX2 and strongly for NANOG, can be propagated clonally on either Matrigel or gelatin, and are morphologically distinct from human PSC progenitors on either substratum but still meet standard in vitro criteria for pluripotency. They form well-differentiated teratomas in immune-compromised mice that secrete human chorionic gonadotropin (hCG) into the host mouse and include small areas of trophoblast-like cells. The cells have a distinct transcriptome profile from the human PSCs from which they were derived (including higher expression of NANOG, LEFTY1, and LEFTY2). In nonconditioned medium lacking FGF2, the colonies spontaneously differentiated along multiple lineages, including trophoblast. They responded to PD173074 in the absence of both FGF2 and BMP4 by conversion to trophoblast, and especially syncytiotrophoblast, whereas an A83-01/PD173074 combination favored increased expression of HLA-G, a marker of extravillous trophoblast. Together, these data suggest that the cell lines exhibit totipotent potential and that BMP4 can prime human PSCs to a self-renewing alternative state permissive for trophoblast development. The results may have implications for regulation of lineage decisions in the early embryo.biological sciences | developmental biology | pluripotent stem cells | totipotent | trophoblast

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“…However, neither CD10 nor calretinin is highly sensitive or specific for mesonephric differentiation, and GATA3 staining has great variability in both intensity and extent. In addition, weak reactivity of GATA3 has been observed in 7% of endometrial carcinomas [16]. Therefore, at this time, there are no antibodies that distinguish MA from Müllerian carcinomas.…”

Section: Discussionmentioning

confidence: 85%

Mesonephric adenocarcinoma of the uterine corpus with intracystic growth completely confined to the myometrium: a case report and literature review

et al. 2017

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Background: Mesonephric adenocarcinoma (MA) is a rare tumor believed to arise from mesonephric remnants occurring mostly in the uterine cervix and, to a lesser extent, the corpus. Since the first case report of MA in the corpus in 1995, only 16 cases have been reported in the English literature. A recent report suggested that MA originates in Müllerian tissue and exhibits the mesonephric differentiation phenotype. Case presentation: An asymptomatic 61-year-old woman was referred to our hospital because of elevated levels of tumor markers. Imaging revealed an intramural lesion of the uterine corpus exhibiting fluorodeoxyglucose uptake. A total hysterectomy and bilateral salpingo-oophorectomy were performed. The tumor was completely confined to the corpus wall and was composed of an intracystic bulky component and an invasive component in the myometrial layer. The tumor exhibited a variety of growth patterns, including a characteristic tubular pattern with dense eosinophilic secretion reminiscent of the thyroid, as well as a variety of morphologies, such as acinar, papillary, and ductal structures. The structures were immunoreactive for CK7, vimentin, CD10, calretinin, PAX8, and GATA3 and almost completely negative for ER/PgR. CA125 and CA19-9 antigen expression was also detected. Conclusion: A case of MA with a unique growth pattern of an intracystic mass within the corpus wall is presented. The histogenesis and differential diagnoses are discussed. The histogenesis of MA is not yet clear. We hypothesize two different pathways involved: 1) direct development from the mesonephric remnants and/or 2) mesonephric transformation of Müllerian adenocarcinoma.

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Semiquantitative GATA-3 Immunoreactivity in Breast, Bladder, Gynecologic Tract, and Other Cytokeratin 7–Positive Carcinomas

Cited by 87 publications

References 19 publications

Comparison of GATA-3, mammaglobin, GCDFP-15 expression in breast carcinoma in serous effusions: A cell-block micro-array study

Comparison of GATA-3, mammaglobin, GCDFP-15 expression in breast carcinoma in serous effusions: A cell-block micro-array study

Heightened potency of human pluripotent stem cell lines created by transient BMP4 exposure

Mesonephric adenocarcinoma of the uterine corpus with intracystic growth completely confined to the myometrium: a case report and literature review

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