The basis 3df2df in Table 1 again contains one set of $functions on all heavy atoms. Second row atoms carry three sets of &functions but first row atoms only two. When this basis set is used instead of 6-31 lG(2dJp) the atomization energies of C10, OC10, and ClOO are all in acceptable agreement with experiment.The basis 3df2df has been applied to two further test molecules and to the C103-isomers. For all species in Table 1 but ClOCl the deviation is less than 5 kcalfmol.The G1 calculations on C103-isomers were started with geometry optimizations on the HF/6-31G*-and MP2f6-31G*-levels of theory. All three supposed isomers, C1000, OC100, and sym-C103 were found as minima [9] on the potential energy hypersurface. The GI total energy calculations using the basis set described above gave heats of formation of 41, 58, and 48 kcalfmol, respectively.From these ab initio calculations it appears that ClOOO might be considered as an intermediate in reaction (1). Since it is a real minimum on the potential energy hypersurface, there must exist a barrier for the exothermic dissociation of ground state ClOOO into C10 and 02.The work was supported in part by the BMFT, Bonn, withinThe authors acknowledge access to the facilities of the Hochstproject 0744120 0.leistungsrechenzentrum Julich. References Howard SidebottomUniversity College Dublin, Ireland Chemical Kinetics f Elementary Reactions f RadicalsRate constants for the gas-phase reactions of ozone with a series of alkenes have been determined using a conventional static system. Ozone loss was monitored in excess of the hydrocarbon and rate data measured at 1 atmosphere total pressure over the temperature range 240-324 K. The Arrhenius parameters obtained are discussed in terms of structure-reactivity relationships. Product studies have been carried out for the reaction of O3 with the conjugated dienes, 1,3-butadiene, 2-methyl-1,3-butadiene (isoprene) and 2,3-dimethyl-1,3-butadiene in air. The results provide information on the decomposition pathways for the primary ozonides and the relative importance of ozone addition to the two doublebonds in isoprene.cant reactivity towards ozone as well as towards hydroxyl and nitrate radicals. Numerous potentially important roles of the ozone-alkene reaction have been recognized since these reactions can provide mutual sinks for both ozone and alkenes and concomitantly serve as sources of partially oxidised compounds e. g. CO, aldehydes, ketones and organic

Background: GATA-3 is a potential marker for detection of metastatic breast carcinoma, reportedly more sensitive than mammaglobin (MAM) and GCDFP-15. We aim to compare the sensitivity of GATA-3, MAM and GCDFP-15 in determining the breast origin of malignant effusions. Methods: Cell blocks from 27 cases of serous effusions positive for metastatic breast cancer were retrieved. Immunohistochemistry for GATA-3, MAM, gross cystic disease fluid protein 15 (GCDFP-15), estrogen receptor (ER) and progesterone receptor (PR) was performed on cell-block micro-array. Statistical analysis using two ways Chi square, one-way ANOVA and multiple regression was performed. Results: The detection rate of breast cancer in serous fluid was significantly higher with GATA-3 (88.8 %, X2 = 15.9, p = 0.00034) than with MAM (51.8 %) and GCDFP-15 (37.0 %). All ER positive cases (19) were GATA-3 positive. Conversely, all GATA-3 negative cases (3) were ER negative. The intensity of stain and percentage of positive cells were significantly higher with GATA-3 (p < 0.0001) than with MAM and GCDFP-15. The intensity and percentage of positive cells score of GATA-3 were statistically associated with ER stain intensity and percentage of positive cell scores.

ObjectiveAlcohol-related liver disease (ALD) ranges from never-decompensated ALD (ndALD) to the life-threatening decompensated phenotype, known as alcohol-related hepatitis (AH). A multidimensional study of the clinical, histological and molecular features of these subtypes is lacking.DesignTwo large cohorts of patients were recruited in an international, observational multicentre study: a retrospective cohort of patients with ndALD (n=110) and a prospective cohort of patients with AH (n=225). Clinical, analytical, immunohistochemistry and hepatic RNA microarray analysis of both disease phenotypes were performed.ResultsAge and mean alcohol intake were similar in both groups. AH patients had greater aspartate amino transferase/alanine amino transferase ratio and lower gamma-glutamyl transferase levels than in ndALD patients. Patients with AH demonstrated profound liver failure and increased mortality. One-year mortality was 10% in ndALD and 50% in AH. Histologically, steatosis grade, ballooning and pericellular fibrosis were similar in both groups, while advanced fibrosis, Mallory-Denk bodies, bilirubinostasis, severe neutrophil infiltration and ductular reaction were more frequent among AH patients. Transcriptome analysis revealed a profound gene dysregulation within both phenotypes when compare to controls. While ndALD was characterised by deregulated expression of genes involved in matrisome and immune response, the development of AH resulted in a marked deregulation of genes involved in hepatocyte reprogramming and bile acid metabolism.ConclusionsDespite comparable alcohol intake, AH patients presented with worse liver function compared with ndALD patients. Bilirubinostasis, severe fibrosis and ductular reaction were prominent features of AH. AH patients exhibited a more profound deregulation of gene expression compared with ndALD patients.

Napsin-A and TTF-1 are both useful markers in distinguishing P-ADC from EP-ADC. However, Napsin-A performed better in poorly differentiated P-ADC and its mimickers. The nuclear staining of TTF-1 is crispier and much easier to interpret than Napsin-A cytoplasmic stain. An antibody panel including TTF-1 and Napsin-A or a dual stain will be very helpful in determining the origin of metastatic adenocarcinoma in serous effusion.

GATA3 is a useful marker in determining the breast origin of metastatic adenocarcinoma in malignant serous effusions. Reactivity to GATA3 may indicate good prognosis. Diagn. Cytopathol. 2016;44:731-736. © 2016 Wiley Periodicals, Inc.